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NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2018

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Sammendrag

Ten elite maize inbred lines were selected based on all over per se performance and gray leaf spot disease reaction. Crosses were made in a 10×10 half-diallel mating design to produce 45 F1 single cross hybrids. The experiment was conducted at Bako national maize research center in 2015 and evaluation of the crosses were made at Bako and Jimma research centers in 2016 by using alpha lattice design with three replications including three commercial checks. All the necessary yield, agronomic and GLS disease data were recorded. In all the studied traits highly significant genotypic differences were observed indicating the existence of genetic variability among the crosses. Analysis of variance for the combining ability indicated GCA and SCA mean squares were significant at (P < 0.001) for all traits except for anthesis-silking interval, ear per plant, ear diameter, lesion length and width. The ratios of GCA/SCA variances for agronomic parameters and all disease parameters were greater than unity except for that of first disease appearance implying the predominance of additive gene actions. Among all inbred lines, P1, P4, P7, P8 and P9 were identified as desirable sources of resistant genes for GLS disease resistance with positive days of first disease appearance and negative disease incidence, severity and AUDPC values for GCA effects. From the analysis of epidemiological data and disease progress curves the Logistic model (R2=96.5) better described the disease progress curves than the Gompertz model (R2=92.5) indicating the presence of delayance in epidemics and the inflection point of the GLS. P1, P7 and P8 were identified as a good general combiners for yield, yield related traits and GLS disease parameters. Thus, these parents were recommended to be used in breeding programs with a purpose of developing high yielder and GLS resistant single cross hybrids. In conclusion this study identified potential high yielding and GLS resistant single cross hybrids (CML-395/CML-383, CML-395/Sc-22, CML-395/CML-197 and CML-383/CML-197). Therefore, it is recommended that these hybrids can be used for direct production where this disease is the most prevalent and/or for further breeding programs in generating novel hybrids for future use.

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Sammendrag

Fourteen advanced maize inbred lines and locally adapted hybrid maize (BH-540) as a check were used to investigate their reaction to GLS disease. Field experiments were conducted at Bako National Maize Research Centre in 2015 and 2016 main plan ng seasons arranged in a randomized complete block design (RCBD) with three replications. Artificial inoculation with Cercospora zeae-maydis was conducted by applying dry, ground, infected maize leaves into the whorls of younger maize plants. Data on agronomic and disease parameters (latent period, disease severity, disease incidence and lesion type) were recorded from the middle two rows. From the combined analysis of variance, maize genotypes showed significant differences with reaction to GLS indicating the existence of genetic variability among the selected genotypes. Highly significant differences were also observed among entries for all agronomic parameters in both seasons. Gray leaf spot incidence and severity varied among genotypes and between years. The mean GLS incidence and severity were higher in 2016 than 2015. GLS disease incidence in two years ranged from 35% on Sc22 to 95% on CML-387 and severity ranged from 15% on A-7016 to 75% on CKL05003. Significant differences in epidemic variability were also observed among genotypes and seasons. From the analysis of disease progress curves Logistic model (R2=94.55) better described the disease progress curves than the Gompertz model (R2=91.50). Parents; P6 and P8 had the most desirable quality for the most of agronomic traits whereas P2, P7 and P9 were the best parents for grain yield. Among all inbred lines, P6, P7 and P14 were iden fied as the most desirable sources of genes for GLS disease resistance. But P6, P7, P8 and P14 were iden fied as the best genotypes in yield, yield related traits and GLS disease parameters. Thus, these parents were recommended to be used in breeding programs with a purpose of developing high yielder and GLS disease resistant open pollinated varieties. In conclusion this study identified potential and promising high yielding and GLS resistant open pollinated genotypes (CKL05017-B-B, CML-395, CML-387, A-7016, Gu o and Sc22). Therefore, it is recommended that these OPVs can be used by resource poor farmers for direct production where this disease is the most prevalent and/or for further breeding programs in generating novel hybrids for future use.