Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2005
Forfattere
Karin Jacobs Brenda D. Wingfield Halvor Solheim Michael J. WingfieldSammendrag
Det er ikke registrert sammendrag
Sammendrag
In spring 2002, extensive damages were recorded in southeast Norway on nursery-grown Norway spruce seedlings that had either wintered in nursery cold storage or had been planted out in autumn 2001. The damages were characterised by leader shoot dieback and necroses on the upper or lower part of the 2001-year-shoot. Gremmeniella abietina and Phomopsis sp. were frequently isolated from the diseased seedlings. RAMS (random amplified microsatellites) profiling indicated that the G.abietina strains associated with diseased nursery seedlings belonged to LTT (large-tree type) ecotype, and inoculation tests confirmed their pathogenicity on Norway spruce. Based on sequence analysis of the internal transcribed spacer (ITS) regions of ribosomal DNA, the Phomopsis strains associated with diseased seedlings do not represent any characterized Phomopsis species associated with conifers. Phomopsis sp. was not pathogenic in inoculation tests, this implying it may be a secondary colonizer. ITS-based real-time PCR assays were developed in order to detect and quantify Gremmeniella and Phomopsis in the nursery stock. We describe here the Gremmeniella - associated shoot dieback symptoms on Norway spruce seedlings and conclude that the unusual disease outburst was related to the Gremmeniella epidemic caused by the LTT type on large pines in 2001.
Forfattere
P. Neville Richard Fischer A.L. Axelsson Dan Aamlid Marco Marchetti T.B. Larsson Annemarie Bastrup-BirkSammendrag
The joint network of tree crown condition monitoring under the EU and ICP Forests operates at two levels, a systematic extensive approach (Level I) based on a 16 km x 16 km trans-national grid of sample plots (>6000 plots) and an intensive approach (Level II) on more than 800 plots across continental Europe. Three ongoing projects embrace the different levels of monitoring, the above mentioned Level I and Level II systems, and the National Forest Inventories (NFIs). All of the three projects are based on a stand structure approach that assumes an increased potential for species diversity with increasing complexity of stand structure. An intensive test-phase of forest biodiversity assessment at more than 100 Level II plots, known as ForestBIOTA is underway during 2005. This project aims to test standardized methods of forest biodiversity assessment in the field and examine the relationship between stand structure, forest deadwood, ground vegetation and epiphytic lichens. A forest classification of the plots is also included. A separate approach, known as BioSoil (due to its combination with a detailed chemical inventory of the soils) is a demonstration project which aims to record indicators of forest biodiversity at the extensive Level I plots. Practical measures of stand structure, including records of tree species, lists of vascular plant species, and simple measures of forest deadwood are included for field assessment during 2006. A pan-European forest type classification elaborating on the EUNIS system and including the Natura 2000 habitat types is proposed. These initiatives are linked to a third project, COMON, operating at the level of the National Forest Inventories aiming to test the same core variables at national levels.
Sammendrag
Intensive monitoring plots of the ICP Forests gathered an amount of data about the ground vegetation in forest ecosystems throughout Europe. Each Country, applying different field techniques, conform to common rules of procedure, under the suggestions of a dedicated Expert Panel which implemented a Unified Coded Flora and comparability targets. Data series are foreseen to contribute to: definition of the forest ecosystem state and changes evaluation; assessment of the specific plant diversity at the ecosystems level. The contribution to scientific knowledge and to Global and Pan-European biodiversity initiatives and networks (ICP-IM, MCPFE, CBD, Forest BIOTA, ALTER-net, etc.) are also underlined. In spite of site-related data, first results (more than 670 plots, with large differences in plant diversity) depict the linkages with temperature, precipitation, dominant tree species and actual soil acidity. Nitrogen deposition seems to have some significant influence, which claims to further studies. Plant data series from ICP Forest’s plot, can be used for on-site confirmation of models including biodiversity k-factors and environment relations.
Forfattere
Halvor SolheimSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Øystein Johnsen Carl Gunnar Fossdal Nina Elisabeth Nagy Jørgen A.B. Mølmann OG Dæhlen Tore SkrøppaSammendrag
Det er ikke registrert sammendrag
Sammendrag
In field experiments, clones of Norway spruce [Picea abies (L.) Karst.] showed different degrees of resistance against pathogenic fungi inoculated into the bark that correlate with differences in polyphenolic parenchyma (PP) cells of the bark. Cells of spruce callus cultures, particularly towards the callus surface, resemble PP cells and this study looks at changes in callus cells during infection and the relative resistance of cultures from clones of low (weak) or high (strong) resistance to fungal infection. Callus cultures, initiated from trees with different resistance, were co-inoculated with Ceratocystis polonica (Siem.) C. Moreau and Heterobasidion annosum (Fr.) Bref. Callus cells from strong clones resemble PP cells of bark tissue from strong clones, having more polyphenolic bodies, while callus cells from weak clones are more similar to PP cells from those clones, which have less extensive phenolic bodies. Callus cultures from trees with weak resistance were more quickly overgrown by both species of pathogenic fungi than cultures from trees with strong resistance. Callus cells of infected cultures showed changes similar to activated PP cells of bark, including enhanced accumulation of polyphenolics. Phenolic bodies were more numerous and more extensive (larger and denser) in callus cells of strong versus weak clones under all conditions. Thus, callus cells may perform similar functions in defense as PP cells in the bark. Callus from trees of varying resistance seem to reflect the relative resistance of the trees from which they are derived, and this study indicates that some mechanisms of resistance can be studied using callus from trees of different resistance.
Sammendrag
Rhizoctonia solani was frequently isolated in the Italian Alps from ursery-grown European beech (Fagus sylvatica) seedlings displaying symptoms of cotyledon rot. Koch?s postulates were verified and mode of infection of the associated isolates was investigated with light and scanning electron microscopy. Population structure of the pathogen was investigated by scoring the anastomosis reaction type in pairings between different isolates from the same seedbed. One pathogen genotype showed a large distribution area within the seedbed, this implying that the inoculum had been building up in the seedbed over a longer time period. Hyphal anastomosis tests and sequence analysis of the internal transcribed spacer (ITS) region of ribosomal DNA indicated that the pathogen belongs to AG 2-1 of R. solani. ITS sequence analysis indicates that the isolates from beech are closely related to R. solani isolates causing a disease on tulip. The striking similarities between the two diseases are discussed.
Sammendrag
*Strawberry Fragaria × ananassa (cv. Korona) was inoculated with Botrytis cinerea by dipping berries in a conidial suspension. *Colonization by the pathogen was monitored using real-time PCR, ELISA and ergosterol assays, the first showing the highest sensitivity. The expression of pathogen B-tubulin and six polygalacturonases (Bcpg1-6) and three host defence genes (polygalacturonase-inhibiting protein (FaPGIP) and two class II chitinases) were monitored using real-time RT-PCR. *The maximum transcript levels of the host defence genes occurred at 16 h postinoculation (hpi) at the presumed initial penetration stage. The unique transcript profile of Bcpg2 over the 96-h incubation time and its high transcript levels relative to those of the other Bcpgs at 8-24 hpi suggest that the gene has a specific role in the penetration stage. *Bcpg1 was expressed constitutively at a relatively high level in actively growing mycelia throughout the experimental period. Comparison of the transcript profiles indicated that Bcpg1 and Bcpg3-6 were coordinately regulated.