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2010

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Sammendrag

The aliphatic biopolyesters cutins and suberins have been suggested to significantly contribute to the stable pool of soil organic matter (SOM), and to be tracers for the above- or belowground origin of plant material. Contrary to other plant-derived aliphatic molecules found in the lipid fraction of soils, the stable isotope derived estimates of turnover of cutins and suberins have never been studied in soils. The aim of this study was to analyse the dynamics of shoot- and root-derived biomarkers in soils using a wheat and maize (C3/C4) chronosequence, where changes in the natural 13C abundance can be used to evaluate the incorporation of new carbon into SOM at the molecular level. The relative distribution of aliphatic monomers in wheat and maize roots and shoots suggested that a,u-alkanedioic acids can be considered as root-specific markers and mid-chain hydroxy acids as shoot-specific markers. The contrasting distribution of the plant-specific monomers in plants and soils might be explained by different chemical mechanisms leading to selective degradation or stabilization of some biomarkers. The changes of the 13C isotopic signatures of these markers with years of maize cropping after wheat evidenced their contrasted behaviour in soil. After 12 years of maize cropping, shoot markers present in soil samples probably originated from old C3 vegetation suggesting that new maize cutin added to soils was mostly degraded within a year. The reasons for long-term stabilization of shoot biomarkers remain unclear. By contrast, maize root markers were highly incorporated into SOM during the first six years of maize crop, which suggested a selective preservation of root biomass when compared to shoots, possibly due to physical protection.

Sammendrag

A second Working Ring Test (WRT) was organised within the framework of the EU/Life+ FutMon Project (`Further Development and Implementation of an EU-level Forest Monitoring System`, LIFE07 ENV/D/000218), to evaluate the overall performance of the laboratories responsible for analysing atmospheric deposition and soil solution samples in European forests, and to verify improvements in the analytical quality resulting from the QA/QC work carried out in the laboratories which participated in previous WRTs organized in the framework of the UN/ECE ICP Forests Monitoring Programme. The WRT was carried out in accordance with International ISO and ILAG guide proficiency test both for sample preparation and numerical elaboration of the results. Five natural atmospheric deposition and soil solution samples and 3 synthetic solutions were distributed to 42 laboratories for analysis using their routine methods for the following variables: pH, conductivity, calcium, magnesium, sodium, potassium, ammonium, sulphate, nitrate, chloride, total alkalinity, total dissolved nitrogen (TDN), dissolved organic carbon (DOC). Two tolerable limits were defined for each variable on the basis of the measured value, the results of previous WRTs, a comparison with the Data Quality Objectives of other international networks, and the importance of the variable in deposition and soil solution monitoring. In the ring test 12% of the results from all the laboratories did not fall within the tolerable limits. This enabled us to identify those variables and laboratories for which improvements in analytical performance are required. The results of the exercise clearly show that the use of data check procedures, as described in the ICP Forests manual for sampling and analysis of atmospheric deposition, makes it possible to detect the presence of inaccurate or outlying results, and would therefore greatly improve the overall performance of the laboratories. A discussion of the improvement of the results in this WRT compared to the previous WRTs is also included, showing a relevant improvent for several variables and underlining the importance of participating to these exercises for the overall analytical quality of the monitoring network.

Sammendrag

Chlorine - one of the most widespread elements on the Earth - is present in the environment as chloride ion or bound to organic substances. The main source of chloride ions is the oceans while organically bound chlorine (OCl) comes from various sources, including anthropogenic ones. Chlorinated organic compounds were long considered to be only industrial products; nevertheless, organochlorines occur plentifully in natural ecosystems. However, recent investigations in temperate and boreal forest ecosystems have shown them to be products of biodegradation of soil organic matter under participation of chlorine. It is important to understand both the inorganic and organic biogeochemical cycling of chlorine in order to understand processes in the forest ecosystem and dangers as a result of human activities, i.e. emission and deposition of anthropogenic chlorinated compounds as well as those from natural processes. The minireview presented below provides a survey of contemporary knowledge of the state of the art and a basis for investigations of formation and degradation of organochlorines and monitoring of chloride and organochlorines in forest ecosystems, which has not been carried out in the Czech Republic yet.

Sammendrag

Concentrations of dissolved organic carbon (DOC) in throughfall and soil solutions at 5, 15 and 40-cm depth were studied in 16 Norway spruce and two Scots pine plots throughout Norway between 1996 and 2006 (Wu et al. 2010a). Average DOC concentrations ranged from 2.3 to 23.1 mg/l and from 1.1 to 53.5 mg/l in throughfall water and soil solutions, respectively. Concentrations of DOC in throughfall and soil waters varied seasonally at most plots with peaks in the growing season. In contrast to reported positive long-term trends in DOC concentrations in surface waters between 1986 and 2003, soil water data from 1996 to 2006 showed largely negative trends in DOC concentrations and no significant trends in throughfall. However, regression analysis for individual sites, particularly at 5- and 15-cm soil depths, showed that DOC concentrations in soil water were significantly and negatively related to non-marine sulphate and chloride. Further studies were carried out on dissolved organic nitrogen (DON, Wu et al. 2010b). Dissolved organic nitrogen (DON) concentrations were significantly and positively correlated to DOC concentrations in throughfall (r2=0.72, p<0.0001) and soil water at 5, 15, and 40 cm (r2=0.86, 0.32, and 0.84 and p<0.0001, 0.04, and <0.0001, respectively). At most sites, the annual median DOC/DON ratio in throughfall ranged from 20.3 to 55.5, while values in soil water were higher, ranging from 24.5 to 81.3 but gradually decreasing with soil depth. DON concentrations varied seasonally in throughfall at many plots and in soil water at 5 cm depth at one plot only, with higher values in the growing season, but there was no noticeable seasonality at greater depth. The ratios of DOC/DON in soil water were significantly positively related to the C/N ratio in soil at the same depth. Above-ground litter input was the main factor having a significant, negative relationship to DOC/DON in soil water at all depths studied. This might reflect the effect of site conditions on both DOC/DON ratios and litter quantity. A comparison of DOC and DON concentrations and fluxes at two Norwegian sites (Birkenes and Hirkjølen) and five Finnish Level II plots (Tammela, Juupajoki, Uusikaarlepyy, Kivalo and Pallasjärvi) showed no obvious correlation between concentrations and site and stand properties such as growing season length, temperature, precipitation, stand age, or soil C or N. DOC concentrations in the O horizon could not be linked to N deposition. However, there were clear within-site seasonal trends, compatible with an effect of temperature on microbial activity.

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Sammendrag

We investigated concentrations of dissolved organic carbon (DOC) in throughfall and soil solutions at 5, 15 and 40-cm depth in 16 Norway spruce and two Scots pine plots throughout Norway between 1996 and 2006. Average DOC concentrations ranged from 2.3 to 23.1 mg/l and from 1.1 to 53.5 mg/l in throughfall water and soil solutions, respectively. Concentrations of DOC in throughfall and soil waters varied seasonally at most plots with peaks in the growing season. By contrast to recently reported positive long-term trends in DOC concentrations in surface waters between 1986 and 2003, soil water data from 1996 to 2006 showed largely negative trends in DOC concentrations and no significant trends in throughfall. However, regression analysis for individual sites, particularly at 5- and 15-cm soil depths, showed that DOC concentrations in soil water were significantly and negatively related to non-marine sulphate (SO4) and chloride (Cl-). The lack of a long-term increase in DOC in soil water in the period May 1996-December 2006 may be due to the relatively small changes in the deposition of SO4 and Cl- in this period.