Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2024
Sammendrag
No abstract has been registered
Sammendrag
Compared to fluctuating soil water (FW) conditions, stable soil water (SW) can increase plant water use efficiency (WUE) and improve crop growth and aboveground yield. It is unknown, however, how stable and fluctuating soil water affect root vegetables. Here, the effects of SW and FW were studied on cherry radish in a pot experiment, using negative pressure irrigation and conventional irrigation, respectively. The assessed effects included agronomic parameters, physiological indices, yield, quality and WUE of cherry radish. Results showed that under similarly average soil water contents, compared with FW, SW increased plant photosynthetic rate, stomatal conductance and transpiration rate, decreased leaf proline content by 13.7–73.3% and malondialdehyde content by 12.5–40.0%, and increased soluble sugars content by 6.3–22.1%. Cherry radish had greater biomass accumulation and nutrient uptake in SW than in FW. Indeed, SW increased radish output by 34.6–94.1% with no influence on root/shoot ratio or root quality. In conclusion, soil water stability affected directly the water physiological indicators of cherry radish and indirectly its agronomic attributes and nutrient uptake, which in turn influenced the crop biomass and yield, as well as WUE. This study provides a new perspective for improving agronomy of root crops and WUE through managing soil water stability.
Sammendrag
We studied the environmental control of shoot growth and floral initiation in annual shoot plants of four red currant cultivars under phytotron conditions. Under natural day length conditions at Ås, Norway (69°40’N), the cultivars ‘Junifer’, ‘Red Dutch’, and ‘Rosetta’ ceased growing and initiated irregular flower formation at 24 and 18°C in late August, indicating a critical photoperiod of approximately 15 h. At 12°C and under outdoor conditions, the processes were delayed by 3-6 weeks depending on the cultivar, indicating a critical photoperiod of approximately 14 h under Nordic field conditions. In 10-h short day (SD), the cultivars ‘Junifer’, ‘Red Dutch’ and ‘Rovada’ ceased growing within 2-3 weeks at 18 and 24°C and within 2-4 weeks at 12°C, followed by irregular floral initiation. However, in 20-h long day (LD), flower initiation was generally scarce, and with contrasting temperature responses among the cultivars, flower initiation was advanced by low temperature in ‘Red Dutch’ and by high temperature in ‘Rovada’ and ‘Junifer’. Flowering performance in the following spring confirmed these results, which demonstrate that red currants are quantitative SD plants with diverse temperature and photoperiod interactions.
Sammendrag
Background: Soil water and organic carbon (C) are key factors affecting the growth and development of apple seedlings. The objective of the study was to investigate the effects of different soil moisture and glucose supplies on apple seedling growth and soil enzyme activities. We hypothesized that the growth of apple seedlings was affected by soil water and C content through their effects on root structure, plant physiological properties and soil enzymatic activities. A pot experiment consisting of nine treatments was set up, including three water treatments with soil moisture contents at 75–85% (normal irrigation, CK), 65–75% (light water stress, LS), and 55–65% (mild water stress, MS) of the soil field capacity, in combination with three glucose treatments with carbon/nitrogen (C/N) ratio of 7.5 (C1, no adding glucose), 10 (C2) and 15 (C3), respectively. Results: Results showed that the LSC2 treatment significantly increased plant height by 7%, stem diameter by 5% and leaf area by 17%, as compared with LSC1. Also, LSC2 significantly increased root dry weight, root vitality and soil enzyme activities. Moreover, results of leaf photosynthetic, malondialdehyde (MDA), peroxidase (POD), superoxide dismutase (SOD) and proline contents also proved that adding glucose improved the drought resistance of plants. Conclusion: LSC2 treatment is more conducive to the growth of apple seedlings, and application of carbon has a good alleviation effect on plant water stress. The study demonstrated that addition of exogenous glucose alleviated light water deficiency, significantly affected root vitality, and promoted apple seedling growth. © 2024 Society of Chemical Industry.
Sammendrag
No abstract has been registered
Forfattere
Joel Abbey David Percival Laura Elina Jaakola Samuel K. AsieduSammendrag
Botrytis blossom blight disease is one of the major challenges to wild blueberry production with annual losses frequently exceeding 20%. In this study, the effect of different fungicide treatments on Botrytis blight development and yield, as well as the mobility and persistence of these fungicides within flower tissues, and fruit of wild blueberries were evaluated under field conditions. This multi-year trial examined five different fungicides (Switch®, Luna Tranquility®, Merivon® Xenium, Propulse®, and Miravis® Prime) each one applied twice at 7-10-day interval. Fungicide quantification in the floral and berry tissues was conducted using a modification of the QuEChErs extraction method and analyzed with GC-MS and HPLC-MS. All the treatments except Switch® reduced disease incidence by over 78 % and severity by over 40 %, compared to the control plots. Switch® and Miravis® Prime reduced both incidence and severity by over 64 % compared to the control plots. Luna Tranquility®, Merivon® Xenium, and Propulse® reduced incidence by at least 47 % and severity by 51 % compared to the control plots. Berry yields were higher in Switch®, Luna Tranquility® and Miravis® Prime treated plots with at least a 19% increase in yield compared to the control plots. The mean concentration of all quantified fungicides was higher in the corolla compared to the gynoecium and the androecium sample areas. Fungicides were persistent and concentrations were sufficient to suppress Botrytis cinerea at fruit set (10 days post application) with no residue detected in harvested berries, except prothioconazole-desthio.
Forfattere
Sonja Keel Alice Budai Lars Elsgaard Brieuc Hardy Florent Levasseur Zhi Liang Claudio Mondini Cesar Plaza Jens LeifeldSammendrag
To increase soil organic carbon (SOC) storage, we need to improve our understanding on how to make best use of available plant biomass. Is it better to leave harvest residues on the field, or can we achieve higher SOC storage after processing biomass through, for instance, composting or pyrolysis to produce biochar? In the present study, we developed new parameters for different types of exogenous organic materials (EOMs), which allowed us to estimate the long-term effect of EOM addition on SOC storage using the soil carbon model RothC. For this purpose, we used a model version that included two additional EOM pools. First, we simulated the SOC evolution after addition of equal amounts of C in plant material and different EOMs (manure, compost, digestate, biochar) for a 38-year cropland trial in Switzerland. As expected, biochar showed the greatest increase in SOC due to its high stability. Next, we estimated how much C would remain after subjecting equivalent amounts of plant material and other EOMs to different processes. Loss rates of C for different processes were obtained from the literature. Due to different decomposition rates, the amounts of C remaining in the EOMs ranged from 7 % for anaerobic digestion of animal excreta to 100 % for plant material added directly to soil. These amounts of C were then added to the soil in the model experiments. Although the largest amount of C is lost during processing to biochar, biochar would clearly lead to highest long-term SOC stocks. Based on these first results we conclude that the trade-off between off-site stabilization and in-soil mineralization does not compromise the use of biochar for soil C storage. This means that despite the high C losses of about 50 % during biochar production, higher amounts of C remain in the soil because biochar has very low decomposition rates. In terms of C sequestration efficiency, biochar thus clearly outperforms the other biomass processing pathways. However, for practical recommendations, additional factors should be considered, such as nutrient availability of EOMs and environmental effects during processing, storage and soil application like nutrient leaching or gaseous emissions. Furthermore, we suggest a full life cycle assessment that considers e.g. energy costs for transport of biomass and energy savings from fossil fuel substitution by natural gas.
Forfattere
Xueqi Li Sujie Zhang Chenyang Wang Bin Ren Fang Yan Shaofang Li Carl Jonas Jorge Spetz Jinguang Huang Xueping Zhou Huanbin ZhouSammendrag
In situ epitope tagging is crucial for probing gene expression, protein localization, and the dynamics of protein interactions within their natural cellular context. However, the practical application of this technique in plants presents considerable hurdles. Here, we comprehensively explored the potential of the CRISPR/Cas nuclease-mediated prime editing and different DNA repair pathways in epitope tagging of endogenous rice (Oryza sativa) genes. We found that a SpCas9 nuclease/microhomology-mediated end joining (MMEJ)-based prime editing (PE) strategy (termed NM-PE) facilitates more straightforward and efficient gene tagging compared to the conventional and other derivative PE methods. Furthermore, the PAM-flexible SpRY and ScCas9 nucleases-based prime editors have been engineered and implemented for the tagging of endogenous genes with diverse epitopes, significantly broadening the applicability of NM-PE in rice. Moreover, NM-PE has been successfully adopted in simultaneous tagging of the MAP kinase (MPK) genes OsMPK1 and OsMPK13 in rice plants with c-Myc and HA tags, respectively. Taken together, our results indicate great potential of the NM-PE toolkit in the targeted gene tagging for Rice Protein Tagging Project, gene function study and genetic improvement.
Forfattere
Alois Bilavčík Stacy Denise Hammond Hammond Jana Fránová Igor Koloniuk Zhibo Hamborg Dag-Ragnar Blystad Milos Faltus Jiri ZamecnikSammendrag
This study aims to establish a cryopreservation protocol for in vitro grown raspberry (Rubus idaeus L.) without the use of dimethyl sulfoxide (DMSO). The protocol exploits cryotherapy for virus eradication in selected favourable genotypes and will facilitate safe preservation of raspberry genotypes in the Czech national cryobank. Raspberries are highly valuable perennial berries grown in temperate countries including the Czech Republic. Raspberry cultivars and elite breeding lines must be maintained as plant collections either in the field or as in vitro cultures. Both maintenance methods are suboptimal primarily because of the many viral and other pathogens transmitted in raspberry field collections, the industriousness, the maintenance costs, and possible somaclonal variation associated with maintenance of in vitro cultures. For these reasons, cryopreservation is currently considered as a prospective method for the long-term preservation of germplasm. One of the best-performing vitrification procedures for plant cryopreservation is based on DMSO solutions. However, due to the potentially mutagenic effect of DMSO, which is contradictory to the principle of germplasm preservation, we focused on testing a DMSO-free procedure using two raspberry varieties. Regrowth rate of the raspberry variety ‘Tulameen’ after application of Plant Vitrification Solution 3 (PVS3) followed by immersion in liquid nitrogen (LN) was 86%. For the raspberry variety ‘Autumn First’, the regrowth level was 73%. In vitro shoots of the cryopreserved variety ‘Tulameen’ were multiplied in a standard cultivation medium and RT-PCR screened for eradication of Black raspberry necrosis virus (BRNV). The eradication rate of BRNV from in vitro cultures of ‘Tulameen’ by cryopreservation was 94%. The eradication of BRNV from control samples, not subjected to LN, was not significantly different (92%). It can be concluded that the DMSO-free tested method is suitable for efficient cryoconservation. Although the LN phase of the cryoprotocol is not required, the PVS3 protocol alone represents a valuable tool for eradication of BRNV from in vitro cultures of tested raspberry shoots. The suggested protocol will be used for the safe backup of healthy plant material.
Forfattere
Jason Lee Anders Marie Louise Davey Bram Van Moorter Frode Fossøy Sanne Boessenkool Erling Johan Solberg Erling Meisingset Atle Mysterud Christer Moe RolandsenSammendrag
Parasitic nematodes are ubiquitous and can negatively impact their host by reducing fecundity or increasing mortality, yet the driver of variation in the parasite community across a wildlife host's geographic distribution remains elusive for most species. Based on an extensive collection of fecal samples (n = 264) from GPS marked moose (Alces alces), we used DNA metabarcoding to characterize the individual (sex, age class) and seasonal parasitic nematode community in relation to habitat use and migration behavior in five populations distributed across a wide latitudinal gradient (59.6°N to 70.5°N) in Norway. We detected 21 distinct nematode taxa with the six most common being Ostertagia spp., Nematodirella spp., Trichostongylus spp., T. axei, Elaphostrongylus alces, and an unclassified Strongylida. There was higher prevalence of livestock parasites in areas with larger sheep populations indicating a higher risk of spillover events. The individual level nematode richness was mostly consistent across study areas, while the number and type of nematode taxa detected at each study area varied considerably but did not follow a latitudinal gradient. While migration distance affected nematode beta-diversity across all sites, it had a positive effect on richness at only two of the five study areas suggesting population specific effects. Unexpectedly, nematode richness was higher in winter than summer when very few nematodes were detected. Here we provide the first extensive description of the parasitic nematode community of moose across a wide latitudinal range. Overall, the population-specific impact of migration on parasitism across the distribution range and variation in sympatry with other ruminants suggest local characteristics affect host-parasite relationships.