Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2023
Abstract
Crown rot, caused by Phytophthora cactorum, is a devastating disease of strawberry. While most commercial octoploid strawberry cultivars (Fragaria × ananassa Duch) are generally susceptible, the diploid species Fragaria vesca is a potential source of resistance genes to P. cactorum. We previously reported several F. vesca genotypes with varying degrees of resistance to P. cactorum. To gain insights into the strawberry defence mechanisms, comparative transcriptome profiles of two resistant genotypes (NCGR1603 and Bukammen) and a susceptible genotype (NCGR1218) of F. vesca were analysed by RNA-Seq after wounding and subsequent inoculation with P. cactorum. Differential gene expression analysis identified several defence-related genes that are highly expressed in the resistant genotypes relative to the susceptible genotype in response to P. cactorum after wounding. These included putative disease resistance (R) genes encoding receptor-like proteins, receptor-like kinases, nucleotide-binding sites, leucine-rich repeat proteins, RPW8-type disease resistance proteins, and ‘pathogenesis-related protein 1’. Seven of these R-genes were expressed only in the resistant genotypes and not in the susceptible genotype, and these appeared to be present only in the genomes of the resistant genotypes, as confirmed by PCR analysis. We previously reported a single major gene locus RPc-1 (Resistance to Phytophthora cactorum 1) in F. vesca that contributed resistance to P. cactorum. Here, we report that 4–5% of the genes (35–38 of ca 800 genes) in the RPc-1 locus are differentially expressed in the resistant genotypes compared to the susceptible genotype after inoculation with P. cactorum. In particular, we identified three defence-related genes encoding wall-associated receptor-like kinase 3, receptor-like protein 12, and non-specific lipid-transfer protein 1-like that were highly expressed in the resistant genotypes compared to the susceptible one. The present study reports several novel candidate disease resistance genes that warrant further investigation for their role in plant defence against P. cactorum.
Authors
May Bente Brurberg Anupam Gogoi Bikal Ghimire Erik Lysøe Mandeep Poudel Håvard Eikemo Jahn Davik Arne StensvandAbstract
No abstract has been registered
Authors
Anupam Gogoi Bikal Ghimire Erik Lysøe Mandeep Poudel Håvard Eikemo Jahn Davik Arne Stensvand May Bente BrurbergAbstract
No abstract has been registered
Authors
Anupam Gogoi Bikal Ghimire Erik Lysøe Mandeep Poudel Håvard Eikemo Jahn Davik Arne Stensvand May Bente BrurbergAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Simeon Rossmann Erik Lysøe Monica Skogen Håvard Eikemo Marta Janiszewska Mirella Ludwiczewska Sylwester Sobkowiak Jadwiga Śliwka May Bente BrurbergAbstract
No abstract has been registered
Authors
Paulina Paluchowska Zhimin Yin Erik Lysøe Simeon Rossmann Mirella Ludwiczewska Marta Janiszewska Sylwester Sobkowiak Håvard Eikemo Monica Skogen May Bente Brurberg Jadwiga ŚliwkaAbstract
No abstract has been registered
Authors
Simeon Rossmann Erik Lysøe Monica Skogen Håvard Eikemo Marta Janiszewska Mirella Ludwiczewska Sylwester Sobkowiak Jadwiga Śliwka May Bente BrurbergAbstract
No abstract has been registered
Abstract
Phytophthora cactorum has two distinct pathotypes that cause crown rot and leather rot in strawberry (Fragaria × ananassa). Strains of the crown rot pathotype can infect both the rhizome (crown) and fruit tissues, while strains of the leather rot pathotype can only infect the fruits of strawberry. The genome of a highly virulent crown rot strain, a low virulent crown rot strain, and three leather rot strains were sequenced using PacBio high fidelity (HiFi) long read sequencing. The reads were de novo assembled to 66.4–67.6 megabases genomes in 178–204 contigs, with N50 values ranging from 892 to 1,036 kilobases. The total number of predicted complete genes in the five P. cactorum genomes ranged from 17,286 to 17,398. Orthology analysis identified a core secretome of 8,238 genes. Comparative genomic analysis revealed differences in the composition of potential virulence effectors, such as putative RxLR and Crinklers, between the crown rot and the leather rot pathotypes. Insertions, deletions, and amino acid substitutions were detected in genes encoding putative elicitors such as beta elicitin and cellulose-binding domain proteins from the leather rot strains compared to the highly virulent crown rot strain, suggesting a potential mechanism for the crown rot strain to escape host recognition during compatible interaction with strawberry. The results presented here highlight several effectors that may facilitate the tissue-specific colonization of P. cactorum in strawberry.