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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2018

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Abstract

Saving water in irrigated agriculture is a high priority in areas with scarce water resources and impacted by climate change. This paper presents results of measurements on water Productivity (WP) under alternative rice growing practices such as alternating wetting and drying,direct seeded rice, modified systems of rice intensification and conventional paddy rice (NI)in two selected districts (Guntur in Andhra Pradesh and Nalgonda in Telangana, India). Under alternative practices, the yields varied from 5.72 to 6.11 t/ha compared with 4.71 t/ha under paddy rice. The average water application varied from 991 to 1494 mm under alternative practices while average application in conventional paddy rice was 2242 mm. Higher yield and lower water application led to an increase in WP varying from 0.45 to 0.59 kg/m3 under alternative practices compared with 0.22 kg/m3 under conventional paddy rice. The measurements showed that less water can be used to produce more crop under alternative rice growing practices. The results are important for water-scarce areas, providing useful information to policy makers, farmers, agricultural departments and water management boards in devising future climate-smart adaptation and mitigation strategies.

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Abstract

In this paper, we outline several recent insights for the priorities and challenges for future research for reducing phosphorus (P) based water eutrophication in the agricultural landscapes of Northwest Europe.We highlight that new research efforts best be focused on headwater catchments as they are a key influence on the initial chemistry of the larger river catchments, and here many management interventions are most effectively made. We emphasize the lack of understanding on how climate change will impact on P losses from agricultural landscapes. Particularly, the capability to disentangle current and future trends in P fluxes, due to climate change itself, from climate driven changes in agricultural management practices and P inputs. Knowing that, future climatic change trajectories for Western Europe will accelerate the release of the most bioavailable soil P. We stress the ambiguities created by the large varieties of sources and storage/transfer processes involved in P emissions in landscapes and the need to develop specific data treatment methods or tracers able to circumvent them, thereby helping catchment managers to identify the ultimate P sources that most contribute to diffuse P emissions. We point out that soil and aqueous P exist not only in various chemical forms, but also in range of less considered physical forms e.g., dissolved, nanoparticulate, colloidal and other particulates, all affected differently by climate as well as other environmental factors, and require bespoke mitigation measures. We support increased high resolution monitoring of headwater catchments, to not only help verify the effectiveness of catchments mitigation strategies, but also add data to further develop new water quality models (e.g., those include Fe-P interactions) which can deal with climate and land use change effects within an uncertainty framework. We finally conclude that there is a crucial need for more integrative research efforts to deal with our incomplete understanding of the mechanisms and processes associated with the identification of critical source areas, P mobilization, delivery and biogeochemical processing, as otherwise even highintensity and high-resolution research efforts will only reveal an incomplete picture of the full global impact of the terrestrial derived P on downstream aquatic and marine ecosystems.

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Abstract

Hepatitis B Virus (HBV) infection can be prevented by vaccination. Vaccines containing the small (S)envelope protein are currently used in universal vaccination programs and achieve protective immuneresponse in more than 90% of recipients. However, new vaccination strategies are necessary for successfulimmunization of the remaining non- or low-responders. We have previously characterized a novel HBVchimeric antigen, which combines neutralization epitopes of the S and the preS1 domain of the large (L)envelope protein (genotype D). The S/preS121–47chimera produced in mammalian cells and Nicotianabenthamiana plants, induced a significantly stronger immune response in parenterally vaccinated micethan the S protein. Here we describe the transient expression of the S/preS121–47antigen in an edibleplant, Lactuca sativa, for potential development of an oral HBV vaccine. Our study shows that oral admin-istration of adjuvant-free Lactuca sativa expressing the S/preS121–47antigen, three times, at 1lg/dose,was sufficient to trigger a humoral immune response in mice. Importantly, the elicited antibodies wereable to neutralize HBV infection in an NTCP-expressing infection system (HepG2-NTCP cell line) moreefficiently than those induced by mice fed on Lactuca sativa expressing the S protein. These results sup-port the S/preS121–47antigen as a promising candidate for future development as an edible HBV vaccine.

Abstract

A large proportion of global agricultural soils contain suboptimal available phosphorus (P) for the growth of many plant species. Boron (B) plays important roles in plant growth and development, but limited research has been conducted to study B uptake under low P availability. This study comprised a hydroponic and a mini-rhizobox experiment with canola (Brassica napus L.), potato (Solanum tuberosum L.) and wheat (Triticum aestivum L.) under P sufficient and deficient conditions. Boron concentrations, rhizosphere soil pH, and gene expression of BnBOR1 in canola were determined. Shoot B concentrations were found significantly increased (11–149%) by low P availability in potato and canola but not in wheat. Reverse transcription polymerase chain reaction (RT-PCR) indicated that BnBOR1;2a, BnBOR1;2c, and BnBOR1;3c were up-regulated after seven days of low P treatment in canola roots. Our results indicate that plant shoot B concentration was dramatically influenced by P availability, and dicots and monocots showed a contrasting B concentration response to low P availability.