Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2001
Forfattere
Malin Elfstrand Carl Gunnar Fossdal Gunilla Swedjemark David Clapham Olof Olsson Folke Sitbon Praveen Sharma Anders Lönneborg Sara von ArnoldSammendrag
In this study we have investigated whether the defensin-like gene spi 1, isolated from Norway spruce, contributes to quantitative disease resistance and is a suitable candidate for utilisation in Norway spruce breeding programmes. The following questions have been raised: (1) Can the putative defense gene, spi 1, improve the defense towards microbial pathogens in a model plant species, tobacco? (2) Is it possible to produce transgenic plants of Norway spruce that overexpress spi 1 and are less susceptible to the pathogenic fungus Heterobasidion annosum? Compared to control plants, tobacco plants expressing spi 1 under an enhanced CaMV 35S promoter permitted less growth of the bacterial pathogen Erwinia carotovora. Embryogenic cultures of Norway spruce were transformed with a similar construct. The general phenotype of regenerated transgenic plants was normal, although it was difficult to maintain certain sublines in culture owing to poor initial growth. Among the transformed plants those with the highest content of SPI 1 displayed reduced fungal growth in the sapwood after inoculation with H. annosum. In conclusion, the spi 1 gene increases resistance in both homologous and heterologous systems.
Forfattere
Jan-Ole SkageSammendrag
Norsk institutt for skogforskning (Skogforsk) i Bergen plantet våren 1999 ut åtte forsøk med omkring 50.000 barrotsplanter fra 76 provenienser av fjelledelgran (Abies lasiocarpa). Plantene etablerte seg meget godt i alle forsøkene. Høydetilveksten første året etter planting var imidlertid ikke tilfredstillende. Fire og fem år etter såing var det allerede store forskjeller i høydeveksten mellom proveniensene. Provenienser fra Arizonia, New Mexico og British Columbia hadde størst høydevekst, mens provenienser fra Colorado, Montana, Wyoming, Utah og Alberta hadde minst høydevekst
Forfattere
Arnstein OrlundSammendrag
Hensikten med dette arbeidet var å undersøke høyde-aldersutvikling i H40-systemet i treslagene gran (Picea abies L. Karst) og sitkagran (Picea sitchensis Bong. Carr.) på Vestlandet basert på målinger i faste forsøksflater i rene kulturbestand. Grunnmaterialet er samlet inn i de 5 kystfylkene fra og med Vest-Agder til og med Møre og Romsdal i årene 1921 fram til 1995. Høydekurvene er beregnet på grunnlag av overhøyde og brysthøydealder. Rutene innen forsøksfelt er slått sammen til en observa-sjonsrekke ved å beregne et gjennomsnitt av overhøyde og brysthøydealder. E tter en slik reduksjon omfatter granmaterialet 77 observasjonsrekker med 381 tilvekstperioder. Materialet for sitkagran består etter samme framgangsmåte av 59 observasjonsrekker med 289 tilvekstperioder. De gjennomsnittlige verdier for overhøyde med standardavvik er beregnet ved begynnelse og slutt for femårs-perioder.
Forfattere
Petter Nilsen Erik Christiansen Halvor SolheimSammendrag
Rapporten tar for seg faren for skader på gjenstående trær og skog etter toppkapping av trær med helikopter langs kraftledningsgater. En økonomisk vurdering av skaden på enkelttrær er også gjort. Liten margborer og liten granbarkbille er to insekter som er vurdert å kunne representere et problem i forbindelse med kapping av topper. Faren for at trær skal dø av angrep av disse billene er liten, og normalt vil de økonomiske tapene ved eventuelt nedsatt tilvekst være små. I dagens situasjon er det imidlertid stor grunn til aktpågivenhet i furuskogsområder, spesielt i østlige områder hvor skogen stedvis er sterkt svekket av soppangrep. En ekstra påkjenning på sterkt avnålede/svekkede trær gjennom billeangrep kan medføre at disse vil dø. I de berørte områdene bør man i nær framtid være aktpågivende ved toppkapping i furuskog. Toppkappede trær av gran blir som regel angrepet av toppråtesopp dersom diameteren er større enn 10 cm, og i liten grad dersom diameteren er mindre enn 5 cm. Råten kan bre seg opptil 40-50 cm pr. år, men stopper gjerne opp 2-4 meter under kappstedet. Langtidserfaringer med råtens fasthet etter toppskader finnes det lite av, men det sannsynlig at deler av veden vil kunne falle utenfor slipkvalitet etter noen ti-år. Problemene i furutrær er vurdert å være langt mindre. Økonomiske vurderinger over en periode på 20 år etter kapping antyder at netto verdiforringelse hos grantrær kan ligge mellom kr. 15.- og 30.- pr. tre avhengig av størrelse før kapping, kostnader, bonitet etc.
Forfattere
Rolf D. Vogt K. Bishop Nicholas Clarke T.C. Gadmar E.T. Gjessing Jan Mulder Michael Starr Dag Olav AndersenSammendrag
The main tasks of the NOMiNiC project (Natural Organic Matter in the Nordic countries; see http://www.kjemi.uio.no/envir/nominic/) is to study the physiochemical characteristics of dissolved organic matter (DOM) and provide reverse osmosis (RO) spring and fall isolates of surface water natural organic matter (NOM) from 5 Nordic forested sites that differ mainly in atmospheric S-loading and climate. Hydrophobic and hydrophilic fractions are hypothesized to be more easily defined and have distinct different chemical characteristics than the total sample. A XAD-8 fractionation may then be used as a simple proxy for the physico-chemical properties of NOM. Both XAD-8 fractions and the total DOM sample have been base and metal titrated. Enhanced understanding of the significance of the numerous operationally defined parameters describing NOM will be achieved through a common multi-dimensional characterisation of the same set of isolates, that span a large spectre of the variation found in DOM. 7 institutes are already collaborating in characterizing the RO isolates using a large range of techniques and new participants are invited. In this presentation the 5 sampling sites are described and some results of the characterisation of the RO isolates are presented.
Forfattere
Malin Elfstrand Carl Gunnar Fossdal Folke Sitbon Olof Olsson Anders Lönneborg Sara von ArnoldSammendrag
Peroxidases constitute a large family of proteins found in all higher plants. Owing to the complexity of the peroxidase isoenzyme family it has been difficult to assess the precise function of individual peroxidase enzymes.In this work we have studied the effects of an endogenous peroxidase-like gene from Norway spruce [Picea abies (L.) Karst], spi 2, on the development and growth of Norway spruce somatic embryo plants.Embryogenic cells of Norway spruce transformed with spi 2 under control of the maize ubi-1 promoter showed up to 40 times higher total peroxidase activity than the control cells; regenerated plants overexpressing spi 2 showed an increased total peroxidase activity.Based on these results and the overall sequence similarity with cationic peroxidases we conclude that spi 2 encodes a peroxidase. Overexpression of spi 2 resulted in increased sensitivity to stress, leading to a reduction in epicotyl formation and in height growth compared with control plants. The plants overexpressing spi 2 also showed a deeper phloroglucinol staining but similar levels of Klason lignin.
Forfattere
Aksel GranhusSammendrag
The risk of logging damage to residual trees (height >3.0 m) and advance regeneration saplings (height 0.5–3.0 m) was evaluated after mechanized (single-grip harvesters + forwarders) and motor-manual (chain saw + skidding) selection harvesting in studies I and II. Harvesting took place during the winter season. Mechanized harvesting caused the highest injury rates, and the difference was highest at high cutting intensity in densely stocked stands. Another important difference between the two operating methods was the spatial distribution of the injury risk relative to striproads. The most important injuries on the larger (>3.0 m) trees were stem- and root wounds, and loss of branches. Wounds tended to be larger, and crown injuries more serious, after mechanized harvesting, but differences were not statistically significant. The most frequent injuries on saplings were crown injuries (loss of branches, stem breakage) and stem lean. In motor-manually harvested stands saplings without pre-harvest deformities in the form of top- or leader defects were more prone to damage than saplings with such defects. A similar difference was not found in stands subjected to mechanized harvesting. This result was attributed to the different work patterns during felling and processing with the two operating methods, in combination with the spatial distribution of saplings of different quality relative to larger trees and stand openings. In study III sapling mortality, and recovery from logging damage in a five-year period after selection harvesting, was investigated. Mortality on the different plots (n=11) was highly variable. For saplings without previous logging damage mortality was related to pre-harvest vigour, and increased with increasing cutting intensity in the immediate surrounding of the sapling. Unspecified site factors also contributed to explain the probability of mortality. Saplings that had been pushed over during harvesting often survived and recovered, while injuries to the crown led to poor survival. Crown injuries were most common on plots subjected to mechanized harvesting, while stem lean was correspondingly important on motor-manually harvested plots. Whether this pattern was attributed to differences in temperature at the time of harvesting (winter), or operating method, is uncertain. In study IV advance regeneration responses in terms of height growth, needle dry weights, and foliar nutrient concentrations were compared after three different release treatments: untouched control, selection harvesting with 50-60 % removal of basal area (BA50-60), and patch cut (25x25 m - 0.063 ha clear-cut). The foliar analyses were carried out five years after treatment, and included dominant and co-dominant (overstorey) trees on control and BA50-60 plots. Height growth and needle dry weights of saplings generally increased with increasing overstorey removal. The growth response was explained by an interaction of foliar nitrogen concentration in current (C) and one-year-old (C+1) needles, and degree of overstorey removal. The foliar analyses did, however, not confirm improved N status after cutting. Increasing overstorey removal led to a reduction of K (C), Mg (C+1) and B (C, C+1) in saplings. A parallel decline of B (C+1) occurred in the overstorey trees (BA50-60). Saplings on control plots had higher concentrations of K and Cu in C-needles, relative to overstorey trees. The influence of neighbour tree basal area on sapling height growth and presence of natural defects (top- and leader damage) was examined in study V. The three stands selected for the study had not been subjected to cutting for several decades, and basal areas ranged from 25–33 m2 ha-1. The relationship between growth and four basal area variables was evaluated: basal area (m2 ha-1) of taller (>3.0 m) neighbour trees within 2.82, 3.99 and 5.64 m radius from the sapling (25, 50 and 100 m2 circular plots), and basal area (m2) of trees within 5.64 m radius weighted according to distance from the sapling. A reduction of growth attributed to increasing basal area of neighbour trees was only observed for the tallest saplings (2.1-3.0 m). Between 33 and 42 % of the saplings had leader- or top defects, and damage frequencies increased with declining distance to the nearest taller neighbour tree.
Sammendrag
Foliage nutrient concentrations of overstorey and understorey Norway spruce (Picea abies (L.) Karst.), and height growth and needle weights of understorey saplings, were studied in a seven-year period after harvest. The following treatments were applied on each of three sites five years prior to needle sampling: control with no cutting, partial cutting with 50-60 % removal of basal area, and patch cut (25 x 25 m - 0.063 ha clear-cut). Saplings on control plots had higher concentrations of K and Cu in current-year needles (C) than overstorey trees. Increased harvest intensity led to a reduction of K in C- needles and Mg in (C+1)-needles of saplings, and of B in both saplings (C, C+1) and overstorey trees (C+1). Sapling needle weights generally increased with harvest intensity, whereas no effect of foliage N status on needle weights could be detected. The growth response of saplings was explained by the interaction between foliage N status, quantified as average N concentration in C- and (C+1)-needles, and harvest intensity. The results illustrate that sufficient N supply is a key factor for the ability of advance regeneration to utilize the improved light condition associated with overstorey removal.
Sammendrag
Injuries and mortality to advance growth (saplings) after selection harvesting was studied in 17 multi-storied Norway spruce (Picea abies (L.) Karst.) stands. Harvest removals ranged from 33 to 67 % of initial basal area. Four of the stands were harvested motor-manually (chain saw + skidding with farm tractors; M-FT). The remaining stands were harvested with single-grip harvesters and forwarders (H-FW). In each stand, injury rates were evaluated on a 24x48 m plot, located between the centrelines of two parallel striproads that were spaced 24 m apart. All logging teams had at least five years of experience in clear-cutting and thinning operations. The trees to be removed, and striproad centrelines, were marked prior to harvest. Mortality varied between 5 and 51 %, whereas total injury (injured + dead saplings) varied between 17 and 76 %. Mortality- and injury levels were generally highest on H-FW plots. Crown reduction and leaning stems were the most frequent types of injury, regardless of operating method. Injury rates increased with sapling height with the H-FW method, whereas the opposite was found on M-FT plots. Saplings without pre-harvest damage in the form of top- or leader defects had a higher probability of being injured than saplings with such damage in stands harvested with the M-FT method. A similar difference was not found on H-FW plots. A logistic regression model show that the spatial risk for injury depends on the interaction between forest condition factors and operational characteristics. Forest condition factors influencing the risk of injury are sapling height and the location of saplings relative to larger residual trees and striproads. Corresponding operational characteristics are operating method and harvest intensity.
Sammendrag
Two zone sites, i.e. two circular areas with 50 km radius, were established in southern Norway. The zone sites were centred in Tofte (the location of a major pulp mill) and in Drammen (the site of a major timber yard). From June to October 2000, 66 forest blocks were visited, 65 of which were situated within the zone site areas. Samples were collected from 40 forest blocks, especially from wood attacked by wood boring insects. At 34 forest blocks, trees of Scots pine, Pinus sylvestris, or Norway spruce Picea abies were provided as trap-logs for Monochamus spp. This material will be sampled in the survey of 2001. Some samples were also taken from a wood chip pile and from imported wood material. The total number of wood samples analysed for nematodes were 275. Out of these, 214 samples were collected from forest trees, stumps, timber and logging wastes of P. sylvestris and P. abies. Three samples contained nematodes belonging to the genus Bursaphelenchus, but the Pine Wood Nematode (PWN), B. xylophilus, was not detected. Similarly, this nematode was not detected in the 10 samples of wood chips, or in the 25 samples of imported lumber or in the 26 samples of imported solid wood packing material. In order reach the minimal number of 3000 samples within reasonable time, the number of samples for the next survey season of 2001 needs to be increased drastically. To achieve this, the sampling will continue within the existing zone sites, and be extended into new zone sites to be established in 2001.