Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2008
Sammendrag
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Sammendrag
Det er ikke registrert sammendrag
Forfattere
Minna MikkolaSammendrag
Public catering in Finland has strong historical roots from the 19th century, connected with the rise of the national state, industrialisation, democracy and modern times in general. The school meal system developed hand in hand with work place meal services, and inherently the aim was to offer lateral support for workers' and pupils' activities by healthy and wholesome nutrition. The public catering had initially a strong label of welfare services and implied economical use of ingredients. Later on, the character of public service of the welfare state was emphasised, as public catering was perceived as a way to promote equality between citizens. The public meal system, and school meal system as part of it, represented not a self-evident and 'natural' developmental path, but can be seen as a result of extensive political, economic and organisational efforts, even fights. Further on, the nutritional and cultural orientations were strengthened when the public school meal system was made a statutory free service for all pupils, first in basic education, and later in secondary education. Today the Finnish welfare state meets the challenge of greying societies and decreasing labour force, and the school meal system, as all public provision systems, in confronted with the trend for increased efficiency and economical operations, including food procurement. Even within these restrictive organisational environments, there is interest in environmentally friendly food and sustainable development by public caterers, municipal officials and politicians of all parties. While the conventional meal system is the prevailing one, there are also movements towards sustainable catering in hundreds of schools around Finland, connected to Education for Sustainable Development (ESD) program. The report is produced within the project “innovative Public Organic food Procurement for Youth”, iPOPY, and will be updated and revised during the project period (2007-2010).
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Margarita Novoa Garrido Cristina L. Marolda Miguel A. Valvano Henning SørumSammendrag
Organic acids are promising candidates as substitutes for antibiotic growth promoters, as they show positive effects on weight gain and control of pathogens. Their addition to the litters as sanitizers, are being suggested. Organic acids affect bacteria in different ways depending on the type of organic acid molecule, external factors such as pH, and the type of bacterial strain. A better understanding of the effects organic acids cause on normal intestinal bacterial flora is needed to make an optimal use of organic acids and avoid possible negative long-term consequences. To investigate the response of a normal intestinal commensal bacterium to continuous mild acid stress, a wild type Escherichia coli strain was submitted to serial passages in buffered medium containing 0,03% formic acid (FA) at mild acidic conditions (pH 5.25 and 6.1) for 20 months. Three morphologically distinctive colony variants (mucoid, non-mucoid and parental) appeared after two months of serial culture in FA. The mucoid colonies formed a long thread that could be up to three meters long when they were picked with a loop. The non-mucoid showed a very dry appearance and irregular shape. The third type of colony variant resembled the parental E. coli strain. Cultivation on Congo red agar and calcofluor suggested the production of fimbriae and/or cellulose or another similar linear extracellular polymer on the surface of the mucoid and non-mucoid variants. In a biofilm assay on glass surfaces the mucoid and non-mucoid variants showed pellicle- and biofilm-forming properties. The parental strain did not show any of the multicellular behaviour features. Phase contrast microscopy demonstrated that the bacterial cells from the mucoid colonies were notorious long, forming "spaghetti"-like structures containing clear vacuoles and pycnotic areas. The SEM examination demonstrated that the cells from the non-mucoid colonies were connected by filament-like structures, probably curli fimbriae, that created a net-like matrix between the bacterial cells which were not observed in colonies from the parental strain or from the mucoid colonies. By LPS extraction and immunoblot analysis we have also shown that the expression of O antigen of the E. coli strain r17 was reduced after several months of weekly passages in the presence of FA. Thus, we also examined the LPS profile of the two colony variants. While the mucoid colonies displayed a normal LPS profile, the non-mucoid variant had an anomalous O antigen profile characteristic of strains lacking the wzz gene, which is responsible for the regulation of the length of the O antigen chains. The development of the parental strain r17 into these morphologically different colonies is likely to be a response of the strain to the environmental stress that the mild acidic pH and the presence of FA in the medium represent. The changes suffered by the parental strain cause biofilm formation. One may speculate whether a selection towards a more invasive and/or biofilm producing bacterial flora is taking place when organic acids are used as feed additives in animal husbandry with the potential consequences for the animal health and hygiene this could represent. Screening of the normal intestinal bacterial microflora of animals fed with these feed additives and their environment could bring more light to this concern. This study has been funded by a grant from the Norwegian Research Council.
Forfattere
Guro BrodalSammendrag
Fusarium head blight (FHB) or scab, seedling blight and foot rot are widespread and destructive diseases of small grain cereals (wheat, barley, oats, rye and triticale) throughout the world. The main causal agents are Fusarium culmorum, F. graminearum, F. avenaceum, F. poae, F. sporotrichioides and Microdochium nivale. More recently also F. langsethiae, previously described as "powdery F. poae", seems to be widespread at least in Scandinavian countries, especially in oats. In addition to causing yield losses, FHB is of great concern because of the potential of these Fusarium species, except M. nivale, to produce a range of secondary metabolites known as mycotoxins in the grain. Mycotoxins cause a potential health risk when contaminated grain is consumed in human and animal food products. The development of FHB is, to a large extent, determined by climatic conditions. It is generally agreed that plants are most susceptible to FHB at anthesis and moist conditions during the anthesis period are important for infection. In hotter regions, F. graminearum is generally regarded as the most important species. In cooler areas, such as Northwest Europe, F. culmorum, F. poae, F. langsethiae and M. nivale has been of greater importance. However, recent investigations indicate that the incidence of F. graminearum has increased considerably in usually cooler regions, including Scandinavian countries. In Norway, a more aggressive and fast growing F. graminearum type was observed in 2004 in the oat cultivar Bessin. A number of seed lots of this cultivar showed severely reduced germination capacity, which was due to a large proportion of infected seed. Mycotoxin analyses of some of the lots showed very high contents of DON (e.g. 25-30 000 ppb). This "new" F. graminearum is now recorded from several areas and also in barley and wheat seed, however, mostly in low frequencies. Nevertheless some serious mycotoxin occurrences have been reported particularly in oats. Research is being carried out to determine if this strain is really new, and if it is likely that it was introduced into Norway by importing infected seeds. Seed health testing of wheat seed in Norway is routinely carried out on agar plates (PDA) and M. nivale and Fusarium spp. are recorded separately. A survey of infection frequencies in Norwegian spring wheat seeds harvested during the years 2000 " 2007 showed that the proportion of M. nivale has decreased whereas Fusarium spp. has increased. In general, the increased occurrence of FHB is believed to be caused by changed cultivation practice, i.e. cereal growing in monoculture over large areas in combination with conservation tillage, which leaves large amount of inoculum on the ground. Possible reasons for the increased occurrences of F. graminearum in cooler regions are the adaptation of the pathogen to cooler conditions and that cooler regions may have gradually become warmer due to possible climate change. Increased FHB is also connected to increased production of maize. Incidence and severity of FHB are often significantly greater when wheat follows maize than when wheat follows other crops. The species responsible for FHB can also cause seedling blight and foot rot. However, the epidemiological relationship between these three diseases is not clear. There is little evidence that seed infection leads to the production of significant quantities of primary inoculum for head blights. More research in this area is needed to understand the role of seed infection in spread and development of FHB. Will it be possible to reduce FHB and the mycotoxin threat with the use of healthy seed? If yes, how is it possible to produce seed without Fusarium?
Sammendrag
In April 2007, orange-red pustules were found in needle scars on defoliated, dead shoots on a nordmann fir (Abies nordmanniana) Christmas tree in Rogaland County in south western Norway. A microscope slide made from the pustules revealed Fusarium-macrospores. On average they were 40.2 µm long and 2.9 µm wide (n=50). No microspores were found. A red coloured culture was obtained by transferring spore mass from a sporodochium to PDA (potato dextrose agar) with a sterile needle. The culture was transferred to SNA (spezieller nährstoffarmer agar) where macrospores developed. The majority of the spores had three septa, most of them were slightly curved, apically bent, basal cells were foot shaped, and spores were long and slender. Six singlespore cultures appeared identical on SNA. Two were chosen for an inoculation test. This test took place on 4 June 2007 in a polyethylene tunnel where the plants were kept during the whole experimental period. Nearly fully grown current year shoots on container grown nordmann fir transplants were inoculated by placing PDA agar plugs with fungal growth at the base of some unwounded needles on each shoot. Twelve transplants were used for each of the two isolates. The plants were covered with polyethylene bags for five days. After the bags were removed, the foliage was kept wet throughout the growing season by 1-3 minutes (depending on temperature) overhead irrigation every third hour. Brown shoots developed during the summer regardless of which singlespore isolate that had been used for inoculation. Samples from brown shoots were collected 20 October. No sporodochia, like we found under field conditions in April, had developed on the inoculated shoots. Neither did they develop after incubation in the laboratory. Only a whitish, fluffy mycelium was seen. CZID (Czapek dox iprodione dichloran agar) was used for reisolation from the dead shoots. From each group of 12 inoculated plants, two and three Fusarium-cultures were obtained, respectively. No symptoms were observed and no Fusarium sp. was isolated from the control plants (exposed to same treatment, but agar plugs with no fungal growth had been used). The Fusarium strain could not be accurately identified to species when the FUSARIUM-ID database v. 1.0 (http://fusarium.cbio.psu.edu) was searched with partial sequence of TEF (translation elongation factor 1-"). The original culture plus the two single spore cultures used for inoculation had identical TEF sequences which were most similar to F. acuminatum (97% identity). One out of six single spore cultures from each of the five reisolates, was sequenced. One of them matched the original culture, but the other four were most similar to F. avenaceum (98% identity). Different from the original culture, the F. av.-like isolates had both macro- and microconidia (0-3 septa, oval spore shape). On PDA, the F. ac.-like culture grew 25 mm in 72 hours (dark and 25°C) and the four F. av.-like cultures grew between 32 to 40 mm. They could not be distinguished by colour. Hyphal coils were found on both species, but were most prominent on the F. ac.-like culture. The fact that sequencing revealed involvement of two Fusarium-species, makes is difficult to draw conclusions about pathogenicity, but since the control plants showed no symptoms, there may be indications that the F. ac.-like fungus killed the inoculated shoots and that the F. av.-like fungus came in as a secondary invader from the environment inside or outside the tunnel. F. acuminatum is reported as a pathogen on conifer seeds and seedlings, but to our knowledge this is the first report of a F. ac.-like fungus causing dieback on a Christmas tree.
Sammendrag
Pine Wood Nematode (PWN, Bursaphelenchus xylophilus) is the causal organism of Pine Wilt Disease (PWD), the worst forest pest of Japan. In Europe PWN is known to exist in Portugal. The Norwegian Food Safety Authority (Mattilsynet) is concerned about the plant health risks and the consequences to the society if PWN should establish in Norway. Mattilsynet needs a scientific assessment of the proposed measures in a contingency plan for PWN. Mattilsynet also needs the risks connected with recent spread of PWN in Portugal to be evaluated before possible changes can be made in the current phytosanitary policy of Norway. On this background Mattilsynet requested a pest risk assessment of PWN from the Norwegian Scientific Committee for Food Safety (Vitenskapskomiteen for mattrygghet, VKM). To answer the request, VKM commissioned a draft pest risk assessment report from the Norwegian Institute for Agricultural Sciences and Environmental Research (Bioforsk). A working group appointed by VKM’s Panel on Plant Health (Panel 9) has been involved during Bioforsk’s work on the report. VKM’s Panel 9 has used the report as a basis for VKM’s opinion. The current document answers Part 1 of Mattilsynet’s request, and was adopted by Panel 9 on a meeting 3rd September 2008. VKM’s Panel 9 gives the following main conclusions of the risk assessment: The PRA area of this assessment is Norway. PWN is not known to occur in Norway. With present trade pattern the probability of entry of PWN into Norway is expected to be high. The most probable pathway for entry of PWN into Norway would be wood packaging material (WPM). The probability that PWN will establish and spread in Norway is considered as high. With regard to the so-called Pest Free Areas (PFAs) of Portugal, the criteria given in ISPM No. 4 (FAO 1995) for establishing and maintaining PFAs have not been met, and the data available is not sufficient to confirm the existence of PFAs. Acceptance of untreated conifer wood from all parts of Portugal will result in a very high probability of entry and a high probability of establishment and spread of PWN and its vector to Norway. Uncertainty factors: To the best of our knowledge PWN is absent from the PRA area. The beetle M. sutor is regarded as a potential vector or PWN, but this has so far not been demonstrated in nature. The currently low vector densities may retard establishment of the PWN and PWD, but it will probably not stop establishment in a longer perspective. Lack of information on the dynamics of PWN populations in cool climates complicates estimates of the spread of the nematode and PWD. Custom routines may fail in their detection of PWN. Import of a seemingly harmless material might therefore pose an unknown risk. WPM follows consignments of all kinds and is a good example of a hazardous material, which often escapes plant health inspections. Detailed assessments of economic consequences of a possible establishment and spread of PWN in Norway, the effects of global warming and other climate changes on the probability for PWD outbreaks, and the effect of possible phytosanitary measures, will be given in Part 2.
Forfattere
Hugh RileySammendrag
Yield results for the last decade are presented from eight long-term trials (started 1977-1991) on loam and clay loams in southeast Norway. Autumn ploughing has been compared with reduced (ploughless) tillage and/or direct drilling. In most cases, spring cereals were grown. Perennial weeds have been sprayed with herbicides when necessary. Fungicides were not used in the trials. In most trials straw residues were chopped and spread. On loam soils, the effects of tillage on yields were mostly similar to those obtained in earlier years. In Trial 1, on loam, mean grain yield was, with spring harrowing only, 95% of that with annual autumn ploughing. It was 96% when harrowing was performed in autumn as well as in spring and 97% when the soil was ploughed every third year. In Trial 2, on loam, positive crop rotation effects were found both with and without ploughing. Reduced tillage, with spring harrowing only, gave 5% lower grain yield than autumn ploughing in this trial also. In Trial 3, on loam, the tillage system did not affect yields of cereals grown in rotation with potatoes, but reduced tillage gave 12% lower potato yield than annual ploughing. In this trial, little difference was found between tillage systems in crop responses to N fertilizer. In Trial 4, on loam, reduced tillage on large-scale (0.7 ha) plots gave 11% lower grain yields than annual ploughing, possibly partly due to shallower sowing depth. In trials on loam soil, the percentage yields obtained without ploughing, relative to those with ploughing, correlated positively with the amount of rainfall in May and with mean air temperature in August. On clay soils. somewhat poorer results have been obtained with reduced tillage in recent years than in previous periods. In Trial 5, on clay loam, the mean grain yield with spring harrowing only, was, over the last ten years, 87% of that obtained with annual autumn ploughing, whereas it was 94% for the whole period since 1991. The inclusion of autumn harrowing increased these figures to 94% and 98%, respectively. Grain protein was lowest with spring harrowing only. Over the last six years, when a treatment with spring ploughing was introduced, this gave 5% lower yield than autumn ploughing. Yields without ploughing, relative to those with ploughing, were negatively correlated with summer rainfall in this trial. In Trial 6, on clay loam, direct drilling has since 1998, as in previous years, given ca. 10% lower yield than autumn ploughing. Autumn harrowing gave 4% lower yield in winter wheat and 6% higher yield in spring oats, than autumn ploughing, whilst yields of spring turnip rape were not affected by tillage. In Trial 7, on clay loam, six alternative straw treatments were compared under various ploughless tillage regimes. Relative to straw removal, retaining large residue amounts depressed yields hardly at all when harrowing was performed in both autumn and spring, but it reduced them by 7% with spring harrowing only and by 13% with direct drilling. The latter system gave on average 18% lower yields than the system with both autumn and spring harrowing. The yield reduction with spring harrowing was 7%. In Trial 8, on silt loam, both direct drilling and spring harrowing of unploughed soil have since 1998 given 6% lower yield than autumn ploughing, whereas both autumn and spring harrowing has given 6% higher yield than ploughing. In this trial, under drier conditions during 1991-1997, unploughed treatments gave up to 11% higher yield than autumn ploughing. Straw retention was beneficial on unploughed treatments during that period, but has had little effect recently.
2007
Forfattere
Odd-Arild FinnesSammendrag
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