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NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2024

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Ochnaflavone is a naturally occurring biflavonoid mainly isolated from Ochna integerrima, manifests health benefits encompassing antidiabetic, anticancer, anti-cardiovascular, and anti-inflammatory activities. However, most bioactivity research has focused on in vitro experiments, rather than in vivo disease models, toxicological assessments, and human clinical trials. Moreover, a comprehensive review of the pharmacological aspects of ochnaflavone is conspicuously lacking. Thus, this review provides a concise and comprehensive summary of existing knowledge on the chemical structure, plant origin, physical properties, biotransformations, and multifaceted biological activities of ochnaflavone along with an in-depth exploration of the complex molecular mechanisms behind these activities, including signaling pathways and gene expression regulation, with the aim of promoting future theoretical needs for ochnaflavone in clinical trials and providing comprehensive insights into the research and application of this valuable natural compound.

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The demand for environmentally friendly, reliable, and cost-effective electrodes for glucose sensor technology has become a major research area in the paradigm shift toward green electronics. In this regard, cellulose has emerged as a promising flexible biopolymer solution with unique properties such as biocompatibility, biodegradability, nontoxicity, renewability, and sustainability. Because of their large surface area and porous structure, fibrous cellulose substrates quickly adsorb and disperse analytes at detection sites. This work focuses on utilizing glyoxal-treated cellulose (derived from brewer’s spent grain (BSG)) for the fabrication of extended gate field-effect transistor (EGFET)-based glucose sensors. This investigation extends to the utilization of BSG-cellulose for glucose detection in biomimicking electrolytes (phosphate buffer saline) to facilitate glucose detection in human blood samples. The fabricated electrode demonstrates a linear range of glucose detection from 1 to 13.5 mM with a Langmuir adsorption coefficient (K) of 0.102. Also, its selectivity toward glucose over interfering molecules such as sucrose, fructose, ascorbic acid, and uric acid under physiological conditions has been demonstrated. This cellulose-based EGFET electrode exhibits a sensitivity of 6.5 μA mM−1 cm−2 with a limit of detection (LOD) of 0.135 mM. Computational studies by density functional theory calculations confirmed the higher binding affinity of glucose molecules with glyoxal-modified cellulose (−0.95 eV) than with pristine cellulose (−0.46 eV). Here, the novelty lies in the fabrication of electrodes with biodegradable catalysts and their integration into the EGFET configuration.

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Future agricultural practices necessitate green alternatives to replace hazardous insecticides while distinguishing between pests and beneficial insects. Chitosan, as a biological macromolecule derived from chitin, is biodegradable and exhibits low toxicity to non-target organisms, making it a sustainable alternative to synthetic pesticides. This review identifies chitosan-derivatives for insecticidal activity and highlights its efficacy including genotoxicity, defense mechanism, and disruption of insect's exoskeleton at different concentrations against several insect pests. Similarly, synergistic effects of chitosan in combination with natural extracts, essential oils, and plant-derived compounds, enhances insecticidal action against various pests was evaluated. The chitosan-based insecticide formulations (CHIF) in the form of emulsions, microcapsules, and nanoparticles showed efficient insecticide action on the targeted pests with less environmental impact. The current challenges associated with the field-trial application were also recognized, by optimizing potent CHIF-formulation parameters, scaling-up process, and regulatory hurdles addressed alongside potential solutions. These findings will provide insight into achieving the EU mission of reducing chemical pesticides by 50 %.

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Black Soldier Fly (Hermetia illucens [L.], Diptera: Stratiomyidae) larvae (BSFL) production from food waste is gaining interest. Food waste, a heterogeneous mix of agro-food and catering leftovers serves as a challenging feedstock for BSF growth due to its varying nutrient composition. BSF, are classified as polyphagous insects with a digestive system featuring midgut for digestion and nutrient absorption. The conversion of food waste by BSFL is heavily influenced by Enterococcus, Klebsiella, Morganella, Providencia, and Scrofimicrobium, which play a vital role in substrate utilization. These microbes determine growth patterns, longevity, oviposition, and egg hatchability, which are intricately tied to the sugar and protein content of their dietary substrates. Pre-treatment techniques including hydrothermal treatment, ionization, pulse electric field discharge, and microbial treatment showed better efficiency in improving the wet waste biomass surface area and waste recovery ratio. In terms of environmental sustainability, a life cycle assessment (LCA) of food waste to BSF conversion facility yields a low global warming potential (GWP) score of 17.36 kg CO2 per ton of functional unit with a significant environmental impact during pre-treatment of food waste at a mass-rearing of BSFL. Therefore, this review emphasizes the digestive system, and gut microbiota of BSFL, with food waste-nutrient utilization by the BSFL. Environmentally promising steps involved in the valorization of food waste resources were evaluated in detail. This review also covers the international regulations involved in food waste fed BSFL, and techno-economic assessment to optimize its valuable nutrients for the new economy in waste management with less environmental footprint.