Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2017
Forfattere
Melissa Magerøy Sharon Jancsik Macaire Man Saint Yuen Michael Fischer Stephen G. Withers Christian Paetz Bernd Schneider John Mackay Joerg BohlmannSammendrag
Acetophenones are phenolic compounds involved in the resistance of white spruce (Picea glauca) against spruce budworm (Choristoneura fumiferiana), a major forest pest in North America. The acetophenones pungenol and piceol commonly accumulate in spruce foliage in the form of the corresponding glycosides, pungenin and picein. These glycosides appear to be inactive against the insect but can be cleaved by a spruce b-glucosidase, PgbGLU-1, which releases the active aglycons. The reverse glycosylation reaction was hypothesized to involve a family 1 UDP-sugar dependent glycosyltransferase (UGT) to facilitate acetophenone accumulation in the plant. Metabolite and transcriptome profiling over a developmental time course of white spruce bud burst and shoot growth revealed two UGTs, PgUGT5 and PgUGT5b, that glycosylate pungenol. Recombinant PgUGT5b enzyme produced mostly pungenin, while PgUGT5 produced mostly isopungenin. Both UGTs also were active in vitro on select flavonoids. However, the context of transcript and metabolite accumulation did not support a biological role in flavonoid metabolism but correlated with the formation of pungenin in growing shoots. Transcript levels of PgUGT5b were higher than those of PgUGT5 in needles across different genotypes of white spruce. These results support a role of PgUGT5b in the biosynthesis of the glycosylated acetophenone pungenin in white spruce.
Forfattere
Merete Wiken Dees Renata Lebecka Juliana Perminow Robert Czajkowski Anna Grupa Agata Motyka Sabina Zoledowska Jadwiga Śliwka Ewa Lojkowska May Bente BrurbergSammendrag
Soft rot and blackleg of potato caused by pectinolytic bacteria lead to severe economic losses in potato production worldwide. To investigate the species composition of bacteria causing soft rot and black leg of potato in Norway and Poland, bacteria were isolated from potato tubers and stems. Forty-one Norwegian strains and 42 Polish strains that formed cavities on pectate medium were selected for potato tuber maceration assays and sequencing of three housekeeping genes (dnaX, icdA and mdh) for species identification and phylogenetic analysis. The distribution of the species causing soft rot and blackleg in Norway and Poland differed: we have demonstrated that mainly P. atrosepticum and P. c. subsp. carotovorum are the causal agents of soft rot and blackleg of potatoes in Norway, while P. wasabiae was identified as one of the most important soft rot pathogens in Poland. In contrast to the other European countries, D. solani seem not to be a major pathogen of potato in Norway and Poland. The Norwegian and Polish P. c. subsp. carotovorum and P. wasabiae strains did not cluster with type strains of the respective species in the phylogenetic analysis, which underlines the taxonomic complexity of the genus Pectobacterium. No correlation between the country of origin and clustering of the strains was observed. All strains tested in this study were able to macerate potato tissue. The ability to macerate potato tissue was significantly greater for the P. c. subsp. carotovorum and Dickeya spp., compared to P. atrosepticum and P. wasabiae.
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Forfattere
Carl Gunnar FossdalSammendrag
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Forfattere
Igor A. Yakovlev Thomas Solvin Harald Kvaalen Arne Steffenrem Marcos Viejo Carl Gunnar FossdalSammendrag
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Forfattere
Raghuram Badmi Abdelhameed Elameen Håvard Eikemo May Bente Brurberg Carl Gunnar Fossdal Paal Krokene Tage ThorstensenSammendrag
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Forfattere
Isabella Børja Kjell Andreassen Jan Čermák Lise Dalsgaard Arthur Gessler Douglas L. Godbold Rainer Hentschel Zachary E. Kayler Paal Krokene Nadezhda Nadezhdina Sabine Rosner Svein Solberg Halvor Solheim Jan Svetlik Mari Mette Tollefsrud Ole Einar TveitoSammendrag
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