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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2024

Abstract

Forest tree seed orchards are artificial populations of genetically superior individuals that play a crucial role in the production of high-quality seeds for reforestation and afforestation programs worldwide. In the pre-genetic-marker era, seed orchards were assumed to act as closed, panmictic populations with equal reproductive success among parents and with no gene flow from external pollen sources. Meeting these assumptions would ensure that the genetic gain attained by breeding would be efficiently transmitted to the next generation, i.e., into seed orchard crops. Many studies published to date have shown that parental reproductive success may be highly variable and that gene flow from undesired pollen sources, a.k.a. pollen contamination, can be substantial. Since the realized genetic gain can be considerably reduced, it is important to monitor mating patterns in seed orchards and thereby control the genetic quality (gain and diversity) of their crops. With the development of genetic markers, the theoretical assumptions as well as the efficiency of measures proposed to enhance desired crosses and reduce pollen contamination in seed orchards could be verified. First attempts to unravel mating patterns and quantify pollen contamination in seed orchards date back to the late 1970s when allozyme markers were introduced. Allozymes remained in use for over two decades, but due to their low resolution, they were gradually replaced with much more powerful microsatellites (SSRs), which, along with the rapid evolution of various statistical approaches, were capable of providing a much more detailed picture of seed orchards’ mating dynamics through pedigree reconstruction. Recently, SNP arrays that have been (and are being) developed for a number of commercially important forest tree species make it possible to affordably and rapidly screen seed orchard seed lots and evaluate the orchards’ genetic efficiency.

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Abstract

Future agricultural practices necessitate green alternatives to replace hazardous insecticides while distinguishing between pests and beneficial insects. Chitosan, as a biological macromolecule derived from chitin, is biodegradable and exhibits low toxicity to non-target organisms, making it a sustainable alternative to synthetic pesticides. This review identifies chitosan-derivatives for insecticidal activity and highlights its efficacy including genotoxicity, defense mechanism, and disruption of insect's exoskeleton at different concentrations against several insect pests. Similarly, synergistic effects of chitosan in combination with natural extracts, essential oils, and plant-derived compounds, enhances insecticidal action against various pests was evaluated. The chitosan-based insecticide formulations (CHIF) in the form of emulsions, microcapsules, and nanoparticles showed efficient insecticide action on the targeted pests with less environmental impact. The current challenges associated with the field-trial application were also recognized, by optimizing potent CHIF-formulation parameters, scaling-up process, and regulatory hurdles addressed alongside potential solutions. These findings will provide insight into achieving the EU mission of reducing chemical pesticides by 50 %.