Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2009
Forfattere
Kjersti Holt HanssenSammendrag
We studied the growth of Norway spruce and Scots pine seedlings in the understory, using three sites with selective cutting and group fellings in SE Norway. Seedlings were planted in plots with and without trenching in gaps of four different sizes, ranging from 20 m2 to 500 m2 and giving light levels from around 20 to 75 % of open sky. After tree years seedlings were dug up and measured. Vegetation cover was also registered. Growth of both species was positively and significantly correlated with increasing gap size. This applied for height, diameter, shoot volume, needle length and needle number as well as total dry weight. However, trenching also had a clear and significant positive effect on the same growth parameters. The growth of some weed species, especially wavy hair-grass (Deschampsia flexuosa) also increased significantly with both gap size and trenching. The results show that competition for both light and below-ground resources matters for understory seedling growth under the studied conditions.
Sammendrag
European ash (Fraxinus excelsior), also known as common ash, occurs naturally inland in lower areas of southeastern Norway and along the southern coast of the country. It is important both as a forest and ornamental tree. During the last decade, dieback has become a disastrous disease on F. excelsior in many European countries. The anamorphic fungus Chalara fraxinea T. Kowalski (1), described for the first time from dying ash trees in Poland, is now considered the cause of ash dieback (2). In May of 2008, C. fraxinea was isolated from 1.5 m high diseased F. excelsior in a nursery in Østfold County in southeastern Norway. Symptoms included wilting, necrotic lesions around leaf scars and side branches, and discoloration of the wood. From symptomatic branches, small pieces (approximately 1 cm3) were excised in the transition area between healthy and discolored wood. After surface sterilization (10 s in 70% ethanol + 90 s in NaOCl), the pieces were air dried for 1 min in a safety cabinet, cut into smaller pieces, and placed on media. The fungus was isolated on potato dextrose agar (PDA) and water agar (WA). On PDA, the cultures were tomentose, light orange, and grew slowly (21 mm mean colony diameter after 2 weeks at room temperature). Typical morphological features of C. fraxinea developed in culture. Brownish phialides (14.8 to 30.0 [19.5] × 2.5 to 5.0 [4.1] μm, n = 50) first appeared in the center of the colonies on the agar plugs that had been transferred. The agar plugs were 21 days old when phialides were observed. Abundant sporulation occurred 3 days later. Conidia (phialospores) extruded apically from the phialides and formed droplets. Conidia measured 2.1 to 4.0 (3.0) × 1.4 to 1.9 (1.7) μm (n = 50). The first-formed conidia from each phialide were different in size and shape from the rest by being longer (6 μm, n = 10) and more narrow in the end that first appeared at the opening of the phialide. Internal transcribed spacer sequencing confirmed that the morphological identification was correct (Accession No. EU848544 in GenBank). A pathogenicity test was carried out in June of 2008 by carefully removing one leaf per plant on 10 to 25 cm high F. excelsior trees (18 trees) and placing agar plugs from a 31-day-old C. fraxinea culture (isolate number 10636) on the leaf scars and covering with Parafilm. After 46 days, isolations were carried out as described above from discolored wood that had developed underneath necrotic lesions in the bark and subsequently caused wilting of leaves. All the inoculated plants showed symptoms, and C. fraxinea was successfully reisolated. No symptoms were seen on uninoculated control plants (eight trees) that had received the same treatment except that sterile PDA agar plugs had been used.
Forfattere
Jarl-Gunnar Salin Peder GjerdrumSammendrag
Wooden poles are frequently used for power and telecommunication lines. After creosote preservation the durability is secured for many decades. This study is related to a Norwegian plant where such poles are produced using Scots pine. Prior to the preservation process the poles have to be dried to a moisture content below the fibre saturation point everywhere in the pole. This is done outdoors in open stacks of poles, i.e. as air-drying. A problem is caused by the difficulties to determine when the target moisture content has been reached. The inventory of drying poles represents considerable value in a company\"s balance sheet and risk of downgrading. Knowledge of drying times during the annual seasons has been insufficient, and no efficient method is known for accurately observing the MC through the various parts of the poles.....
Forfattere
Carl Gunnar Fossdal Igor A. Yakovlev Ari M. Hietala Halvor SolheimSammendrag
To identify differentially expressed genes of the white-rot fungus Heterobasidion parviporum subtractive cDNA libraries were constructed using suppressive subtraction hybridization (SSH) technique with RNA extracted from an advanced stage of decay area and from colonization front next to the reaction zone of the stem of a mature Norway spruce trees. Besides cytochrome P450s and proteins with unknown function, the SSH libraries constructed contained genes involved in basic cellular processes, andcell wall degradation. To examine the role of selected candidate genes three trees, showing a variable degree of wood decay, were used for real-time RT-PCR profiling of candidate genes. In the decay transition areas the study revealed activity centers that showed remarkable similarity in the transcript profiles of monitored genes.
Forfattere
Igor A. Yakovlev Harald Kvaalen Carl Gunnar Fossdal Øystein JohnsenSammendrag
MicroRNAs (miRNAs) are endogenous small RNAs that can have large-scale regulatory effect and could participate in epigenetic regulation of gene expression in plants. We show for the first time that temperature during zygotic embryogenesis and seed maturation in Norway spruce regulates an “epigenetic memory” in the progeny, regenerated through somatic embryogenesis. The warmer the in vitro temperature applied, the later the regenerated plants formed terminal buds in the common environment the second growth season. The differences were very large, and similar in size to a provenance separation of 4 – 6 degrees of latitude (Kvaalen and Johnsen, 2008). To study a molecular mechanisms of a memory from embryo development we have prepared two concatemerized small RNA libraries representing small RNAs predominantly expressed in plants growing from seeds obtained after embryogenesis in cold environment (CEL) and warm environment (WEL) after short day (SD) treatment (going to bud set). In total we obtained 201 different small RNAs, with dominated length of 21-nt, 123 from WEL and 93 from CEL. Using multiple methods, including BLAST, sequence alignment and sequence folding we found 27 novel candidate miRNAs and only 3 earlier described. Additionally 103 small RNAs have exact matches in spruce EST database, which could be their putative targets and 67 small RNAs have no matches. We used quantitative RT–PCR to study the expression patterns of 31 chosen candidate miRNAs and monitor the occurrence stage-dependent miRNA-mediated cleavage for 4 regions of putative mRNA targets. All miRNA show difference in transcript levels after SD treatment and 12 miRNAs show constitutively differential expression in progeny from CE and WE. Kvaalen H, Johnsen O (2008) Timing of bud set in Picea abies is regulated by a memory of temperature during zygotic and somatic embryogenesis. New Phytologist 177: 49-59
Forfattere
Svein Olav HolienSammendrag
Det er ikke registrert sammendrag
Forfattere
Ola Wågbø Oddmund HjukseSammendrag
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Forfattere
Ellen Marie Forsberg Sigrid Hagerup Melhuus Svenn Arne Lie Ragnhild Kongsvoll Mads SvennerudSammendrag
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Sammendrag
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Forfattere
Kjell Andreassen Volkmar Timmermann Nicholas Clarke Halvor Solheim Ingvald Røsberg Wenche AasSammendrag
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