Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2006
Forfattere
Fride Høistad ScheiSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
2005
Forfattere
Jan BjerketvedtSammendrag
Det er ikke registrert sammendrag
Forfattere
Harald Kvaalen Ola Gram Dæhlen Anne Tove Rognstad Borgny Sveen Grønstad Anne Eskild Nilsen Ulrika EgertsdotterSammendrag
Det er ikke registrert sammendrag
Forfattere
Kjell AndreassenSammendrag
Det er ikke registrert sammendrag
Forfattere
Harald Kvaalen Ola Gram Dæhlen Anne Tove Rognstad Borgny Sveen Grønstad Ulrika EgertsdotterSammendrag
Seeds of Abies lasiocarpa (Hook.) Nutt. (subalpine fir) were dissected, and the different parts were analyzed for elemental composition. The data were used to design a novel growth medium for initiation of somatic embryogenesis. Embryogenic cultures were initiated from immature zygotic embryos from six open-pollinated families of A. lasiocarpa on three different media.The frequency of initiation was the highest in early to mid-July when the zygotic embryo explants were ca. 0.8 mm long. Thereafter the response declined rapidly.The culture media did not significantly affect the initiation frequencies, but the subsequent growth and culture survival was dependent on the culture medium. On the Schenk Hildebrandt medium, many cultures ceased to grow and died. Several of the decaying cultures were rescued after transfer to one of the new media. Proliferating cultures could be stimulated to produce mature embryos. Of 2510 mature somatic embryos, 212 (8.4%) converted to plants, and 35 plants have grown over two periods.
Forfattere
Nina Jøhnk Ari M. Hietala Carl Gunnar Fossdal Mari-Anne Newman David B. Collinge Harald KvaalenSammendrag
Stilbene synthases make the backbone of stilbenes in a single enzymatic step. Many stilbenes are stressinduced antimicrobial phenolics, believed to work in disease resistance. In conifers, stilbenes are found in pine (Pinus), spruce (Picea) and a few other genera.Stilbene synthase isoforms in pine use cinnamyl-CoA to form pinosylvin, these are termed pinosylvin synthases, whereas stilbene synthases in spruce use pcoumaryl- CoA to form resveratrol and are sometimes termed resveratrol synthases.Pinosylvin has been found to be more effective than resveratrol in inhibiting fungal growth and wood decay (Seppnen et al. 2004), and pathogens of non-pinosylvin producing species have been found to be less tolerant of pinosylvin than pine pathogens (Seppnen et al. 2004). In the present study, Norway spruce (Transformation of Norway spruce with the pinosylvin synthase gene, PSS1) was transformed using the biolistic technique with a gene encoding pinosylvin synthase, PSS1, from Scots pine and the E. coli nptII antibiotic resistance gene.Vector constructs carrying PSS1 in sense and antisense, as well as control vectors without PSS1 were transferred into two embryogenic cell lines of Norway spruce, 11703-B63 and 186-3C. Selection condition for transgenic tissue was conferred by nptII in combination with the antibiotic geneticin. Geneticin resistant lines were recovered from all transformation events, a total of 55 lines.NptII was detected by PCR analysis in many of these lines, the majority derived from the cell line 11703 B63. However, nptII protein was detected in just five lines, and several lines of evidence indicate that the transgenic lines obtained in this study might be chimaeras.Fifty-six seedlings were successfully regenerated from antibiotic resistant lines, 50 of these were derived from cell line 11703 B63. All seedlings died during cold storage before further testing could be carried out.
2004
Forfattere
Jan BjerketvedtSammendrag
Det er ikke registrert sammendrag
Forfattere
Erlend Ystrøm Haartveit Dag FjeldSammendrag
Det er ikke registrert sammendrag
2003
Forfattere
Jan Bjerketvedt Øystein Dale Erlend Ystrøm Haartveit Jørn Kristian Lileng Albert WinsentsSammendrag
Det er ikke registrert sammendrag