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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2016

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The effects of daylength and temperature on flowering of the cultivated octoploid strawberry (Fragaria × ananassa Duch.) have been studied extensively at the physiological level, but information on the molecular pathways controlling flowering in the species is scarce. The flowering pathway has been studied at the molecular level in the diploid short-day woodland strawberry (F. vesca L.), in which the FLOWERING LOCUS T1 (FvFT1)–SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (FvSOC1)–TERMINAL FLOWER1 (FvTFL1) pathway is essential for the correct timing of flowering. In this work, we show by transgenic approach that the silencing of the floral repressor FaTFL1 in the octoploid short-day cultivar ‘Elsanta’ is sufficient to induce perpetual flowering under long days without direct changes in vegetative reproduction. We also demonstrate that although the genes FaFT1 and FaSOC1 show similar expression patterns in different cultivars, the regulation of FaTFL1 varies widely from cultivar to cultivar and is correlated with floral induction, indicating that the transcription of FaTFL1 occurs at least partially independently of the FaFT1–FaSOC1 module. Our results indicate that changing the expression patterns of FaTFL1 through biotechnological or conventional breeding approaches could result in strawberries with specific flowering and runnering characteristics including new types of everbearing cultivars.

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Professional and scientific networks built around the production of sweet cherry (Prunus avium L.) led to the collection of phenology data for a wide range of cultivars grown in experimental sites characterized by highly contrasted climatic conditions. We present a dataset of flowering and maturity dates, recorded each year for one tree when available, or the average of several trees for each cultivar, over a period of 37 years (1978–2015). Such a dataset is extremely valuable for characterizing the phenological response to climate change, and the plasticity of the different cultivars’ behaviour under different environmental conditions. In addition, this dataset will support the development of predictive models for sweet cherry phenology exploitable at the continental scale, and will help anticipate breeding strategies in order to maintain and improve sweet cherry production in Europe.

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The European plum cultivar 'Opal', widely grown in Scandinavia, frequently initiates too many flowers and set too many fruits. If excess fruitlets remain on the trees until harvest, the crop consists of small, unmarketable fruits of low fruit quality and return bloom will be reduced. The current study investigated effects of different crop loads established at blossom and fruitlet stages on yield, fruit quality and return bloom. For two seasons starting in 2008 on mature 'Opal'/'St. Julien A' trees, two crop loads 50 and 25% flowers reduced were established at full bloom and at 10-12 mm fruitlet size and compared with an un-thinned control treatment. Treatments were applied on single whole trees in a randomized complete block design with six replications. Spindle trees spaced at 3.0×5.0 m were selected for uniformity in growth habit, flowering intensity and trunk cross sectional area. Final fruit set varied from 63% on the control trees to 18% when thinned at bloom. Yield was negatively correlated with the fruit set response. Thinning at the fruitlet stage resulted in smaller fruits at the same crop level compared to flower thinning. Fruit quality parameters characterized by bright yellow skin background colour, red surface colour and the concentrations of soluble solids increased significantly as the crop load was reduced. Other fruit quality parameters like percentage acidity were not significantly different and did not show a clear response to the thinning. Return bloom was promoted most when trees were thinned at bloom the year before.

Sammendrag

European plum cultivar Jubileum (Prunus domestica L.) blooms abundantly most years and too many fruit can be set if flowers and/or fruitlets are not properly thinned. In 2007, 2008 and 2009, mature ‘Jubileum/St. Julien A’ trees were treated with ethephon either at full bloom, at concentrations of 250, 375 and 500 mg/l or when fruitlets averaged ~12 mm in diameter at concentrations of 125, 250 and 375 mg/l. In general, flower-thinning treatments reduced fruit set significantly. Fruit set decreased with increasing ethephon concentrations, and the highest rate of ethephon applied either at full bloom (500 mg/l) or post bloom (375 mg/l) resulted in excessive over-thinning. Up to 375 mg/l of ethephon was required at full bloom whereas only 125 mg/l of ethephon was required post bloom for marked fruitlet thinning. Yields confirmed the fruit set response and yield reductions were significant. In most years, all thinning treatments resulted in fruit larger than 38 mm in diameter compared to fruit from unthinned control trees. Fruit quality, characterized by blue surface color and soluble solids content was generally higher and increased significantly with the reduction in crop load. Fruit firmness of fruit from all ethephon applications was lower than that of the fruit from unthinned control trees. In contrast, titratable acidity did not show a clear response to ethephon thinning. Return bloom the following year was mostly unaffected by all ethephon applications compared to the control. In conclusion, an ethephon application at a rate of up to 375 mg/l applied at full bloom will result in adequate thinning of ‘Jubileum’ plums and achieved a target of about 10- 15 % reduction in fruit set. When weather conditions are not conducive during flowering, a post bloom ethephon application at 125 mg/l may be applied however, this should only be considered in years of excessive flowering and as a last resort.

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When establishing an apple orchard it is important to stimulate the growth of young trees to fill up their allotted space in the row and minimize the time to come into full production with high quality fruit. In May 2012 two-year-old 'Summerred'/'M.9' knip trees were planted at the experimental farm at NIBIO Ullensvang, western Norway. The planting distance was 4×1 m (2500 trees ha-1). Three fertigation schemes were established (zero; low - 0.15 g N m-1 row day-1, and high nitrogen - 0.30 g N m-1 row day-1) and two crop loads allowed (low and high). In the year after planting the crop loads were limited to zero and five apples tree-1, respectively. In the third leaf the crop loads were adjusted by hand to 15 apples and 30 apples tree-1, respectively. The experimental design was a randomized blocks with four replications and 10 trees treatment-1. No significant effects of N-fertigation on soil NO3-, NH4+ or N-min contents were found. However, N-fertigation increased the leaf nitrogen content the whole season and leaf N were ≥ than the standard adequacy range (1.5-2.0%). Trees reached the desirable height of 3 m in the third leaf. Trunk circumference and number of branches per tree increased from second to third leaf, but were not significantly influenced by the N-fertigation. In the third leaf the high crop load trees yielded 7 kg tree-1 (17.5 t ha-1) and the low crop load 4 kg tree-1. Fruit weights were negatively correlated with the crop loads. Small differences in fruit quality attributes were found. Return bloom was not affected after the second leaf. However, the high crop load in the third leaf clearly reduced the amounts of return bloom in 2015, irrespective of the amount of N-fertigation.

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Prohexadione-Ca, an acylcylohexadione, is a plant growth retardant jointly developed by BASF, Germany and Kumiai Chemical Industry, Japan. In 2012, a trial on 11-year-old 'Aroma'/'M 9' apple trees growing on the experimental farm at NIBIO Ullensvang, western Norway was initiated. Trees were treated with prohexadione-Ca (trade name Regalis®, 10% prohexadione-Ca as active ingredient) 10 days after full bloom (June 6) at concentrations of 125 or 250 g ha-1; other trees were also sprayed 10 days after full bloom and again one month later (July 5) at the same concentrations and compared against an untreated control. Treatments were applied to individual whole trees in a randomized complete block design with four replications. Prohexadione-Ca treatments significantly reduced shoot growth of treated apple trees. Two treatments with 125 g or one treatment of 250 g reduced growth by ~80%. Prohexadione-Ca had little effect on yield and fruit quality. Shoot growth was also retarded in the year following application and this is attributed to a carry over effect of the prohexadione-Ca application(s).

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Triploid apple cultivars are often favored by human selection due to their increased fruit size, even though they possess higher requirements in terms of pollination compared to their diploid counterparts. However, similar to the sensory characteristics, preferences for the size and shape of apple fruit are not universal, but culturally dependent. In order to investigate the influence of selection pressure on the frequency of triploid genotypes among different traditional apple germplasms, 141 apple accessions maintained in ex situ collections in Norway and 115 traditional and international apple accessions from Bosnia and Herzegovina (B&H) were analyzed using 8 microsatellite markers. Genotypes possessing more than two alleles per locus were deemed to be triploid. The results of SSR analyses from this and comparable studies indicate that the triploid apple cultivars are much more frequent among the traditional apple germplasm from Southern Europe than they are among the traditional apple germplasm from Northern Europe. The possible causes for these results are also discussed.

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The response of three-year-old 'Summerred' trees to crop load regulation by the chemical thinning agent Brevis® was tested in comparison with different levels of hand thinning. Brevis® was applied once or twice at a dosage of 1.1 and 2.2 kg ha-1 when fruitlets were approximately 15 and 19 mm in diameter at the time of the first and second application, respectively. All Brevis® applications significantly reduced the number of fruits tree-1. Both the single application of 2.2 kg ha-1 and the two-fold application of 1.1 kg ha-1 thinned the tree to the target crop load level of 30 apples tree-1. The degree of thinning by the single application of 1.1 kg ha-1 Brevis® did not significantly increase fruit weight and resulted in a very strong inhibition of return bloom similar to that observed in the untreated controls and trees hand thinned to 60 fruits tree-1. Return bloom was positively related to the level of thinning obtained. At the target fruit load of about 30 apples tree-1, return bloom was around 40% of the bloom in the year of the thinning trial. A further thinning to circa 15 fruits tree-1 increased return bloom to approximately 60%. In conclusion, 'Summerred' trees planted as two-year-old feathered trees should not bear more than 20 to 25 fruits tree-1 in their 3rd leaf in a northern climate to avoid the onset of biennial bearing and to ensure regular annual yields. Only then sufficient numbers of flower buds will develop to have the chance to obtain a good crop in the next year.