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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2019

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Sammendrag

Plant biology in Norway. Some main aspects; 1. Major efforts on micro and macro algae are now ongoing in Norway (lots of funding goes this way) 2. The pure basic plant biology research with molecular aspects are mostly at the major universities (exemplified here by Prof. Grini and Haman and in smaller groups at other institutions (exemplified by the TOPPFORSK project in epigenetics at NIBIO). 3. A lot of the plant biology in Norway is related to evolution, biodiversity and ecology in general, including climate change (Exemplified by studies in clinal variation and phenology) 4. There is a lot of applied research related to feed and food crops as well as forestry (including invasive species. abiotic stress, plant pathogen interactions insects and fungi with importance for agriculture). 5. There is a National Network for Plant Biology Research in Norway (led by Paul Grini from UiO). This network holds annual/biannual Norwegian Plant Biology conference (NorPlantBio) conferences. 6. Examples from the various institutions in Norway will now be presented.

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Shallot (Allium cepa var. aggregatum) is an important vegetable crop belonging to the genus Allium. The present study attempted to develop an efficient droplet-vitrification cryopreservation method for shallot ‘10603’ shoot tips. In vitro stock shoots were maintained on Murashige and Skoog (1962) medium (MS) supplemented with 30 g L-1 sucrose, 0.5 mg L-1 BAP, 0.1 mg L-1 NAA and 8 g L-1 agar (pH=5.8). Shoot tips (2.0-3.0 mm in length) were excised from 4-week-old stock shoots and stepwise precultured with increased sucrose concentrations from 0.3 to 0.5 M, each concentration for 1 day. The precultured shoot tips were then loaded for 20 min with a solution composed of 2 M glycerol and 0.5 M sucrose, before exposure to PVS3 for 3 h at room temperature. Dehydrated shoot tips were transferred onto aluminum foils (2×0.8 cm), prior to direct immersion into liquid nitrogen (LN) for cryostorage. For thawing, frozen aluminum foils were moved from LN and immediately transferred into unloading solution composed of liquid MS containing 1.2 M sucrose. After incubation at room temperature for 20 min, shoot tips were post-cultured on solidified MS medium containing 0.3 M sucrose for 2 days and then transferred onto a recovery medium for shoot regrowth. With this procedure, 94% shoot tips survived, and 58% shoot tips regenerated into shoots following cryopreservation.