Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2016
Forfattere
Tage ThorstensenSammendrag
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Forfattere
Tage ThorstensenSammendrag
Det er ikke registrert sammendrag
Forfattere
Tage ThorstensenSammendrag
Det er ikke registrert sammendrag
Forfattere
Tage ThorstensenSammendrag
Det er ikke registrert sammendrag
Forfattere
Tage ThorstensenSammendrag
Det er ikke registrert sammendrag
Forfattere
Tage ThorstensenSammendrag
Det er ikke registrert sammendrag
Forfattere
Tage ThorstensenSammendrag
Det er ikke registrert sammendrag
Forfattere
Tage ThorstensenSammendrag
Det er ikke registrert sammendrag
Forfattere
Rasmus John Normand Frandsen Silas A. Rasmussen Peter B. Knudsen Silvio Uhlig Dirk Petersen Erik Lysøe Charlotte H. Gotfredsen Henriette Giese Thomas O. LarsenSammendrag
Biosynthesis of the black perithecial pigment in the filamentous fungus Fusarium graminearum is dependent on the polyketide synthase PGL1 (oPKS3). A seven-membered PGL1 gene cluster was identified by over-expression of the cluster specific transcription factor pglR. Targeted gene replacement showed that PGL1, pglJ, pglM and pglV were essential for the production of the perithecial pigment. Over-expression of PGL1 resulted in the production of 6-O-demethyl-5-deoxybostrycoidin (1), 5-deoxybostrycoidin (2), and three novel compounds 5-deoxybostrycoidin anthrone (3), 6-O-demethyl-5-deoxybostrycoidin anthrone (4) and purpurfusarin (5). The novel dimeric bostrycoidin purpurfusarin (5) was found to inhibit the growth of Candida albicans with an IC50 of 8.0 +/− 1.9 μM. The results show that Fusarium species with black perithecia have a previously undescribed form of 5-deoxybostrycoidin based melanin in their fruiting bodies.
Forfattere
Erik Lysøe Rasmus J.N. Frandsen Hege Divon Valeria Terzi Luigi Orrù Antonella Lamontanara Anna-Karin Kolseth Kristian F. Nielsen Ulf ThraneSammendrag
Fusarium langsethiae is a widespread pathogen of small grain cereals, causing problems with T-2 and HT-2 toxin contamination in grains every year. In an effort to better understand the biology of this fungus, we present a draft genome sequence of F. langsethiae Fl201059 isolated from oats in Norway. The assembly was fragmented, but reveals a genome of approximately 37.5 Mb, with a GC content around 48%, and 12,232 predicted protein-coding genes. Focusing on secondary metabolism we identified candidate genes for 12 polyketide synthases, 13 non-ribosomal peptide synthetases, and 22 genes for terpene/isoprenoid biosynthesis. Some of these were found to be unique compared to sequence databases. The identified putative Tri5 cluster was highly syntenic to the cluster reported in F. sporotrichioides. Fusarium langsethiae Fl201059 produces a high number of secondary metabolites on Yeast Extract Sucrose (YES) agar medium, dominated by type A trichothecenes. Interestingly we found production of glucosylated HT-2 toxin (Glu-HT-2), previously suggested to be formed by the host plant and not by the fungus itself. In greenhouse inoculations of F. langsethiae Fl201059 on barley and oats, we detected the type A trichothecenes: neosolaniol, HT-2 toxin, T-2 toxin, Glu-HT-2 and numerous derivatives of these.