Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2012
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Forfattere
Gage Koehler Jens Rohloff Robert Wilson Per Winge Anita Sønsteby Muath Alsheikh Stephen K, RandallSammendrag
There is limited research about variations between cultivars for freezing tolerance in fruit crops and indeed much less is known about the distinctive in ground structure of the crown that herbaceous perennials depend on for regeneration in spring. Because strawberry is a representative species for the Rosaceae crops knowledge gained is expected to be transferrable to benefit improvement of many of these related crops. To gain insight into the molecular basis that may contribute to overwintering hardiness, a comparative proteomic analysis was carried out for four Fragaria x ananassa (octoploid strawberry) cultivars that differ in freezing tolerance. Protein expression was investigated in the overwintering relevant crown structure of strawberry from plants exposed to 0, 2, and 42 day cold treatments and were examined by two-dimensional gel electrophoresis (2D). Some proteins, such as molecular chaperones, antioxidants / detoxifying enzymes, metabolic enzymes, and pathogenesis related proteins were revealed to be at significantly higher levels before cold exposure in the most freezing tolerant cultivars, (‘Jonsok’ and ‘Senga Sengana’) compared to the least tolerant cultivars (‘Frida’ and ‘Elsanta’). Freezing tolerance was evaluated for the cultivars before and after cold exposure, with findings that support that the most freezing tolerant cultivars are poised for rapid adaptation to cold exposure, suggesting potential differences in capacity or rate for cold acclimation. Thus, the molecular basis for enhanced overwintering survival may be related to the elevated basal level of a number of proteins, many of which are known to confer stress tolerances. These findings are presented and overlaid with a LFQP shotgun analysis and microarray analysis. This study presents the largest quantitative proteomic data-set for strawberry crown tissue during cold exposure to date. Through the comparison of these cultivars that differ in freezing tolerance, proteins that may contribute to cold tolerance but lack significant cold induction were revealed.
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Forfattere
Gage Koehler Robert Wilson John V. Goodpaster Anita Sønsteby Xianyin Lai Frank A. Witzmann Jin-Sam You Jens Rohloff Stephen K, Randall Muath Alsheikh-YousefSammendrag
To gain insight into the molecular basis contributing to overwintering hardiness, a comprehensive proteomic analysis comparing crowns of Fragaria × ananassa (octoploid strawberry) cultivars that differ in freezing tolerance was conducted. Four cultivars were examined for freeze tolerance and the most cold-tolerant cultivar (‘Jonsok’) and least tolerant cultivar (‘Frida’) were compared with a goal to reveal how freezing tolerance is achieved in this distinctive overwintering structure and to identify potential cold-tolerance associated biomarkers. Supported by univariate and multivariate analysis, a total of 63 spots from 2DE analysis and 135 proteins from label-free quantitative proteomics (LFQP) were identified as significantly differentially expressed in crown tissue from the two strawberry cultivars exposed to 0, 2, and 42 day cold treatment. Proteins identified as cold tolerance associated included molecular chaperones, antioxidants/detoxifying enzymes, metabolic enzymes, pathogenesis related proteins and flavonoid pathway proteins. A number of proteins were newly identified as associated with cold tolerance. Distinctive mechanisms for cold tolerance were characterized for two cultivars. In particular, the ‘Frida’ cold response emphasized proteins specific to flavonoid biosynthesis, while the more freezing tolerant ‘Jonsok’ had a more comprehensive suite of known stress responsive proteins including those involved in antioxidation, detoxification, and disease resistance. The molecular basis for ‘Jonsok’ enhanced cold tolerance can be explained by the constitutive level of a number of proteins that provide a physiological stress-tolerant “poise”.
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