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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2023

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Abstract

Red raspberry (Rubus idaeus L.) is an economically valuable soft-fruit species with a relatively small (~300 Mb) but highly heterozygous diploid (2n = 2x = 14) genome. Chromosome-scale genome sequences are a vital tool in unravelling the genetic complexity controlling traits of interest in crop plants such as red raspberry, as well as for functional genomics, evolutionary studies, and pan-genomics diversity studies. In this study, we developed genome sequences of a primocane fruiting variety (‘Autumn Bliss’) and a floricane variety (‘Malling Jewel’). The use of long-read Oxford Nanopore Technologies sequencing data yielded long read lengths that permitted well resolved genome sequences for the two cultivars to be assembled. The de novo assemblies of ‘Malling Jewel’ and ‘Autumn Bliss’ contained 79 and 136 contigs respectively, and 263.0 Mb of the ‘Autumn Bliss’ and 265.5 Mb of the ‘Malling Jewel’ assembly could be anchored unambiguously to a previously published red raspberry genome sequence of the cultivar ‘Anitra’. Single copy ortholog analysis (BUSCO) revealed high levels of completeness in both genomes sequenced, with 97.4% of sequences identified in ‘Autumn Bliss’ and 97.7% in ‘Malling Jewel’. The density of repetitive sequence contained in the ‘Autumn Bliss’ and ‘Malling Jewel’ assemblies was significantly higher than in the previously published assembly and centromeric and telomeric regions were identified in both assemblies. A total of 42,823 protein coding regions were identified in the ‘Autumn Bliss’ assembly, whilst 43,027 were identified in the ‘Malling Jewel’ assembly. These chromosome-scale genome sequences represent an excellent genomics resource for red raspberry, particularly around the highly repetitive centromeric and telomeric regions of the genome that are less complete in the previously published ‘Anitra’ genome sequence.

Abstract

The oilseed rape Ms8xRf3, developed by BASF Agricultural Solutions Seed US LLC, is a fertile hybrid tolerant to glufosinate-ammonium containing herbicides. The hybrid is derived through conventional breeding of the male sterile oilseed rape event Ms8 and the oilseed rape event Rf3, called the fertility restorer. Ms8 and Rf3 were produced by Agrobacterium tumefaciens mediated transformation of cells from a conventional oilseed cultivar. The dominant gene for male sterility in event Ms8 is barnase, and the dominant gene for fertility restoration in event Rf3 is barstar. The bar gene, conferring tolerance to glufosinateammonium, is found in both Ms8 and Rf3. The scientific documentation provided in the renewal application for the genetically modified oilseed rape events Ms8, Rf3 and Ms8 x Rf3 is adequate for risk assessment, and in accordance with EFSA guidance on risk assessment of genetically modified plants for use in food or feed. The VKM GMO panel does not consider the introduced modifications in events Ms8, Rf3 and Ms8 x Rf3 to imply potential specific health or environmental risks in Norway, compared to EU-countries.