Paal Krokene
Research Professor
Attachments
Min CVBiography
I study plant-insect interactions, and particularly how conifers defend themselves against bark beetles and fungi. The work is highly interdisciplinary and I collaborate with chemists, molecular biologists and ecologists at NIBIO and abroad. The methods we use span from field experiments to chemical ecology and molecular biology. I have worked at NIBIO since 1992. I studied at the University of Oslo, where I graduated as Master of Science in ecology / entomology in 1992 and Dr. Scient. (PhD) in forest entomology in 1996. Since 2004, I have been an adjunct professor at the Norwegian University of Life Sciences (NMBU), where I teach forest entomology. Since 2018, I am also a member of the Plant Health Panel of the Norwegian Scientific Committee for Food and Environment (VKM).
Authors
Tao Zhao Suresh Ganji Christian Schiebe Björn Bohman Philip Weinstein Paal Krokene Anna-Karin Borg-Karlson C. Rikard UneliusAbstract
Convergent evolution of semiochemical use in organisms from different Kingdoms is a rarely described phenomenon. Tree-killing bark beetles vector numerous symbiotic blue-stain fungi that help the beetles colonize healthy trees. Here we show for the first time that some of these fungi are able to biosynthesize bicyclic ketals that are pheromones and other semiochemicals of bark beetles. Volatile emissions of five common bark beetle symbionts were investigated by gas chromatography-mass spectrometry. When grown on fresh Norway spruce bark the fungi emitted three well-known bark beetle aggregation pheromones and semiochemicals (exo-brevicomin, endo-brevicomin and trans-conophthorin) and two structurally related semiochemical candidates (exo-1,3-dimethyl-2,9-dioxabicyclo[3.3.1]nonane and endo-1,3-dimethyl-2,9-dioxabicyclo[3.3.1]nonane) that elicited electroantennogram responses in the spruce bark beetle Ips typographus. When grown on malt agar with 13C D-Glucose, the fungus Grosmannia europhioides incorporated 13C into exo-brevicomin and trans-conophthorin. The enantiomeric compositions of the fungus-produced ketals closely matched those previously reported from bark beetles. The production of structurally complex bark beetle pheromones by symbiotic fungi indicates cross-kingdom convergent evolution of signal use in this system. This signaling is susceptible to disruption, providing potential new targets for pest control in conifer forests and plantations.
Abstract
1 The European spruce bark beetle Ips typographus is a damaging pest on spruce in Europe. Beetle interactions with tree species originating outside the natural range of the beetle are largely unknown and may be unpredictable because trees without a co-evolutionary history with the beetle may lack effective defences. 2 The terpenoid composition and breeding suitability for I. typographus of the historic host Norway spruce Picea abies were compared with two evolutionary naïve spruces of North American origin that are extensively planted in North-West Europe: Sitka spruce Picea sitchensis and Lutz spruce Picea glauca x lutzii. 3 The bark of all three species had a similar chemical composition and similar levels of total constitutive terpenoids, although Norway spruce had higher total induced terpenoid levels. 4 Beetles tunnelling in the three spruce species produced similar amounts of aggregation pheromone. Controlled breeding experiments showed that I. typographus could produce offspring in all three species, with a similar offspring length and weight across species. However, total offspring production was much lower in Sitka and Lutz spruce. 5 Overall, the results of the present study suggest that I. typographus will be able to colonize Sitka and Lutz spruce in European plantations and in native spruce forests in North America if introduced there.
Abstract
The purpose of this study is to increase the basic understanding of outbreak dynamics in order to improve the management of bark beetle outbreaks. The spruce bark beetle Ips typographus is a major disturbance agent of European forests and is the continent’s most economically and environmentally damaging bark beetle. Outbreaks of the spruce bark beetle are often triggered by large windfall episodes, and we have utilized a unique opportunity to study a Slovakian outbreak where little salvage logging was performed in some areas after a 2.5 million m3 storm-felling in 2004. Our analyses focused on the first five years after the windfall, and we used a combination of empirical data and simulation models to understand the spatial patterns of beetle-killed forest patches developing during the outbreak. The univoltine beetle population used an increasing proportion of the windfelled trees during the two first seasons after the storm, but from the third season onwards our comparisons of inter-patch distance distributions indicated a transition from beetle production largely in windfall areas to a self-sustaining outbreak with infestation patches developing independently of the windthrows. The size of new infestation patches formed after this transition was modeled as a function of beetle pressure, estimated by the proportion of a circle area surrounding new patches that was covered by infestation patches the previous year. Our model results of patch size distribution did not correspond well with the empirical data if patch formation was modeled as a pure dispersal–diffusion process. However, beetle aggregation on individual trees appears to be important for patch development, since good correspondence with empirical data was found when beetle aggregation was incorporated in the modeled dispersal process. The strength of correspondence between the beetle aggregation model and the empirical data varied with the density of aggregation trees in the modeled landscape, and reached a maximum of 83% for a density of three aggregation trees per infestation patch. Our results suggest that efficient removal of windfelled trees up until the start of the second summer after a major windfall is important to avoid a transition into a patch-driven bark beetle outbreak that is very difficult to manage. Our results also indicate that the outcome of a patch-driven outbreak is difficult to predict, since the development of new infestation patches is not a simple function of beetle pressure but is also affected by beetle behavior and local forest conditions.
Authors
Paal KrokeneAbstract
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Abstract
In 2024, spruce bark beetle (Ips typographus) catches decreased in all counties except Telemark, Sør-Trøndelag, and Nordland. The highest catches this year were observed in Telemark and Buskerud. In Telemark, the catches are the highest recorded since the major spruce bark beetle outbreak that started in the mid-1970s. In Buskerud, while catches have declined compared to last year, they remain historically high. In Oppland, the catches have decreased markedly from the record-breaking year of 2023 but remain at moderately high levels. Across Southern Norway, this year’s catches are slightly above the 46-year average. The relatively high catches in Buskerud and Oppland are likely a delayed response to the storm damage in November 2021, as municipalities heavily affected by the storm report especially high catches. Additionally, field reports in 2024 indicate attacks on standing trees in areas with much windfall after the 2021 storm. Many of these damage reports likely pertain to trees attacked by beetles in 2023 or earlier but that are only now showing visible symptoms. The 2024 bark beetle season was characterized by extremely dry and warm weather in May, followed by a very wet summer with normal to slightly below-average temperatures. The warm May weather coincides with the beetles' primary flight period, favoring beetle dispersal and egg-laying. Additionally, the dry conditions in May may have stressed spruce trees, reducing their resistance to beetle attacks. The wet weather later in the summer likely benefited the trees while being sub-optimal for the beetles. Overall, the weather conditions during the 2024 season were probably relatively favorable for the beetles. A temperature-based development model estimate that, by September 17, the spruce bark beetle could have completed two generations near the Oslofjord, along the southern coastline, and in low-lying inland valleys. However, these model results do not necessarily mean that the beetles completed two generations in 2024 but indicate that conditions were warm enough to make it possible.
Authors
Rylee Isitt Andrew M. Liebhold Rebecca M. Turner Andrea Battisti Cleo Bertelsmeier Rachael Blake Eckehard G. Brockerhoff Stephen B. Heard Paal Krokene Bjørn Økland Helen F. Nahrung Davide Rassati Alain Roques Takehiko Yamanaka Deepa S. PureswaranAbstract
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Authors
Ngan Bao Huynh Paal Krokene Jorunn Elisabeth Olsen Taina Pennanen Adriana Puentes Vaida Sirgedaitė-Šėžienė Vytautas Čėsna Ieva Čėsnienė Melissa MagerøyAbstract
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