Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2023
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Trygve S. AamlidAbstract
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Sebastian EiterAbstract
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Geir-Harald StrandAbstract
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Ana-Maria Madalina Pantazica Andre van Eerde Mihaela-Olivia Dobrica Iuliana Caras Irina Ionescu Adriana Costache Catalin Tucureanu Hege Særvold Steen Catalin Lazar Inger Heldal Sissel Haugslien Adrian Onu Crina Stavaru Norica Branza-Nichita Jihong Liu ClarkeAbstract
The recent SARS-CoV-2 pandemic has taught the world a costly lesson about the devastating consequences of viral disease outbreaks but also, the remarkable impact of vaccination in limiting life and economic losses. Vaccination against human Hepatitis B Virus (HBV), a major human pathogen affecting 290 million people worldwide, remains a key action towards viral hepatitis elimination by 2030. To meet this goal, the development of improved HBV antigens is critical to overcome non-responsiveness to standard vaccines based on the yeast-produced, small (S) envelope protein. We have recently shown that combining relevant immunogenic determinants of S and large (L) HBV proteins in chimeric antigens markedly enhances the anti-HBV immune response. However, the demand for cost-efficient, high-quality antigens remains challenging. This issue could be addressed by using plants as versatile and rapidly scalable protein production platforms. Moreover, the recent generation of plants lacking β-1,2-xylosyltransferase and α-1,3-fucosyltransferase activities (FX-KO), by CRISPR/Cas9 genome editing, enables production of proteins with “humanized” N-glycosylation. In this study, we investigated the impact of plant N-glycosylation on the immunogenic properties of a chimeric HBV S/L vaccine candidate produced in wild-type and FX-KO Nicotiana benthamiana. Prevention of β-1,2-xylose and α-1,3-fucose attachment to the HBV antigen significantly increased the immune response in mice, as compared with the wild-type plant-produced counterpart. Notably, the antibodies triggered by the FX-KO-made antigen neutralized more efficiently both wild-type HBV and a clinically relevant vaccine escape mutant. Our study validates in premiere the glyco-engineered Nicotiana benthamiana as a substantially improved host for plant production of glycoprotein vaccines.
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Ruben Erik Roos Johan Asplund Tone Birkemoe Aud Helen Halbritter Rechsteiner Siri Lie Olsen Linn Vassvik Kristel van Zuijlen Kari KlanderudAbstract
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Daniel Beat Müller Miguel Las Heras Hernandez Avijit Vinayak Pandit Anne Falk Øgaard Kjell Inge ReitanAbstract
Phosphorus is a building block for all life and therefore plays an essential role in food production. Currently, large amounts of phosphorus enter the Norwegian food system from abroad in the form of mineral fertilizer, feedstuff, food, as well as micro-ingredients for animal feed, mainly in salmon farming. However, only a small fraction of this phosphorus ends up as food for humans, while the largest part accumulates in soil and water systems. This inefficiency entails two challenges: 1. Phosphorus supply is critical. Phosphate rock, the primary source of phosphorus for fertilizer and micro-ingredient production, is a limited resource that is highly concentrated in a few countries. Over 80% of global phosphate rock reserves are found in only 5 countries, and ~70% are located in Morocco and Morocco-occupied Western Sahara. The high concentration renders many countries vulnerable to geopolitical and economic instabilities and threatens food safety. The EU has therefore included phosphate rock on its list of Critical Raw Materials. 2. The accumulation of phosphorus in water systems can lead to eutrophication and dead zones, threatening fish stocks and other aquatic life. The high phosphorus concentration in soils due to overfertilization over long periods of time increases the danger of losses to water systems by runoff, further exacerbating the eutrophication risk. A more circular use of phosphorus could simultaneously reduce supply and pollution risks. This is particularly relevant in Norway, where the government has an ambition to increase salmon and trout production from currently 1,5 to 5 million tons by 2050. Achieving a circular phosphorus economy is a complex task: (i) The land- and the sea-based food systems are increasingly interlinked, for example through agricultural production of fish feed or the application of fish sludge on agricultural land. (ii) The Norwegian phosphorus cycle is increasingly interlinked with that of other countries as trade flows along the entire food supply chain are growing. (iii) Phosphorus fertilizers, both primary and recycled, are often contaminated with heavy metals such as cadmium, uranium, and zinc, which tend to accumulate in soils. Cleaning the phosphorus cycle is therefore vital for soil fertility and human health. This report is based on the MIND-P project, which studied the Norwegian phosphorus cycle for both agriculture and aquaculture at a farm-by-farm basis and explored options for increasing circularity. The project identified farm-level and structural barriers to managing phosphorus resources more effectively. We propose four fundamental strategies to overcome these barriers: 1. Develop and maintain a national nutrient accounting. 2. Minimize phosphorus losses and accumulations at farm level. 3. Establish infrastructures for capturing, processing, trade, and use of manure and fish sludge to produce high-quality recycled fertilizers that are tailored to the needs of the users in Norway and abroad. 4. Adopt a regulatory framework to promote a market for recycled fertilizer. The strategies proposed here were developed with the support of an Advisory Panel consisting of representatives from government, industry, industry associations, and NGOs in an online and two physical workshops conducted in 2022.