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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2022

Abstract

This book presents evidence-based research on climate-neutral and resilient farming systems and further provides innovative and practical solutions for reducing greenhouse gas emissions and mitigating the impact of climate change.Intensive farming systems are a significant source of greenhouse gas emissions, thereby contributing to global warming and the acceleration of climate change. As paddy rice farming is one of the largest contributors, and environmentally damaging farming systems, it will be a particular focus of this book. The mitigation of greenhouse gas emissions needs to be urgently addressed to achieve the 2°C target adopted by COP21 and the 2015 Paris Agreement, but this is not possible if local and national level innovations are not accompanied by international level cooperation, mutual learning and sharing of knowledge and technologies. This book, therefore, brings together international collaborative research experiences on climate-neutral and resilient farming systems compiled by leading scientists and experts from Europe, Asia and Africa. The chapters present evidence-based research and innovative solutions that can be applied or upscaled in different farming systems and regions across the world. Chapters also present models and technologies that can be used for practical implementation at the systemic level and advance the state of the art knowledge on carbon-neutral farming. Combining theory and practice, this interdisciplinary book provides guidance which can inform and increase cooperation between researchers from various countries on climate-neutral and resilient farming systems. Most importantly, the volume provides recommendations which can be put into practice by those working in the agricultural industry, especially in developing countries, where they are attempting to promote climate-neutral and resilient farming systems. The book will be of great interest to students and academics of sustainable agriculture, food security, climate mitigation and sustainable development, in addition to policymakers and practitioners working in these areas.

To document

Abstract

Giant panda could have bamboo as their exclusive diet for about 2 million years because of the contribution of numerous enzymes produced by their gut bacteria, for instance laccases. Laccases are blue multi-copper oxidases that catalyze the oxidation of a broad spectrum of phenolic and aromatic compounds with water as the only byproduct. As a “green enzyme,” laccases have potential in industrial applications, for example, when dealing with degradation of recalcitrant biopolymers, such as lignin. In the current study, a bacterial laccase, Lac51, originating from Pseudomonas putida and identified in the gut microbiome of the giant panda’s gut was transiently expressed in the non-food plant Nicotiana benthamiana and characterized. Our results show that recombinant Lac51 exhibits bacterial laccase properties, with optimal pH and temperature at 7–8 and 40°C, respectively, when using syringaldazine as substrate. Moreover, we demonstrate the functional capability of the plant expressed Lac51 to oxidize lignin using selected lignin monomers that serve as substrates of Lac51. In summary, our study demonstrates the potential of green and non-food plants as a viable enzyme production platform for bacterial laccases. This result enriches our understanding of plant-made enzymes, as, to our knowledge, Lac51 is the first functional recombinant laccase produced in plants.