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NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2019

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Sammendrag

Laboratory screening tests are commonly used to indicate wood materials’ resistance or susceptibility to surface mould growth, but the results can deviate from what happens during outdoor exposure. In this study, the aim was to investigate how well agar plate screening tests and water uptake tests can predict mould growth on exterior wooden claddings. The tested wood materials included Norway spruce heartwood (Picea abies), sapwood and heartwood of Scots pine (Pinus sylvestris), aspen (Populus tremula), acetylated Radiata pine (Pinus radiata) and DMDHEU-modifed Scots pine sapwood. The agar plate test included four inoculation methods (two monoculture spore suspensions of Aureobasidium species, one mixed-culture spore suspension, and inoculation from outdoor air) and three incubation temperatures (5, 16 and 27 °C). Inoculation method and incubation temperature had signifcant efects on the mould rating in the agar plate screening test, but none of the agar plate test combinations gave good indications of outdoor performance. Results from the agar plate test gave signifcantly negative correlations or no signifcant correlation with results from the outdoor test. However, the water uptake test gave signifcantly positive correlations with outdoor mould rating, and could be a useful indicator of susceptibility of uncoated wooden claddings to surface mould growth.

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This study addresses changes in visual appearance of unpainted wood materials exposed outdoors. Specimens of aspen (Populus tremula), Norway spruce (Picea abies), untreated Scots pine (Pinus sylvestris), DMDHEU-modified Scots pine and acetylated Radiata pine (Pinus radiata) were exposed facing south in Ås, Norway for 62 weeks. During this period, mould growth coverage, lightness (L*) and the uniformity of the weather grey colour were assessed. Mould growth coverage was evaluated visually using a rating system. L* and the uniformity were evaluated using image analysis. The increase in mould rating of the wood materials developed in varying speed, but all specimens had reached the maximum rating after 42 weeks. Until then, the changes in L* correlated significantly with the mould rating. However, the specimens continued to darken after they had reached maximum mould rating. DMDHEU was the only material that obtained a more uniform colour as a consequence of the weathering.

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Svandalsfossen, som er en velkjent og hyppig besøkt turistattraksjon i Sauda kommune, påvirker ved høy vannføring trafikksikkerheten på fylkesvei 520 gjennom fossesprut som hindrer sikt. På grunn av den tidvis utfordrende trafikksituasjonen vurderes det nå tiltak for å begrense fossesprut på veien, enten ved å (1) sprenge vekk en fjellnabbe i fossen for å redusere fossesprut og/eller endre dens retning, eller (2) installere en vegg ved veien på sørsiden av fossen for å fysisk skjerme veien mot fossesprut. Samtidig er det registrert be-tydelige naturverdier ved Svandalsfossen: den rødlistede mosen kystfloke (Heterocladium wulfsbergii) og en fossesprøytsone (E05) med A-verdi (svært viktig) etter DN håndbok 13, som i dag omfatter de rødlistede naturtypene fosseberg og fosse-eng. Derfor undersøkte vi utbredelsen av kystfloke og rødlistede naturtyper ved Svandalsfossen og vurderte kon-sekvensene de ulike inngrepsalternativene vil ha for naturverdiene. Kystfloke ble funnet langs store deler av fossen, og det ble i tillegg oppdaget en annen rødlistet mose-art kyst-skeimose (Platyhypnidium lusitanicum), men begge forekom i områder som i liten grad vil påvirkes av de foreslåtte inngrepene. De rødlistede naturtypene fosseberg og fosse-eng fant vi derimot i tilknytning til inngrepsområdet. Disse naturtypene er helt avhengige av høy fuk-tighet og det forventes at en reduksjon i fosserøyk/-sprut vil ha tydelig negativ innvirkning på dem. Derfor frarådes det å sprenge vekk fjellmateriale i fossen og dermed redusere fosse-røyk/-sprut. Installasjon av en vegg endrer ikke tilførsel av fuktighet for naturverdiene ved Svandalsfossen og vil dermed ha langt mindre negativ innvirkning, særlig hvis den bygges av gjennomsiktig materiale som ikke skygger ut vegetasjonen.

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We distinguish five Xanthomendoza species in Norway, viz., X. borealis, X. fallax, X. fulva, X. oregana, and X. ulophyllodes, based on morphology and molecular evidence. This paper gives an updated taxonomy of the Norwegian species of Xanthomendoza, and addresses previous misconceptions. Xanthomendoza ulophyllodes is reported as occurring in Norway. The species was previously misunderstood in Norway and removed from the Nordic checklist. We show that the nuclear internal transcribed spacer (nrITS) is a useful barcode marker for the treated species. We provide a key and short descriptions of the species, with notes on specific issues, ecology, geographic distribution, illustrations, maps, and a DNA reference library (DNA barcoding).

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High-throughput sequencing is increasingly favoured to assay the presence and abundance of microRNAs (miRNAs) in biological samples, even from low RNA amounts, and a number of commercial vendors now offer kits that allow miRNA sequencing from sub-nanogram (ng) inputs. Although biases introduced during library preparation have been documented, the relative performance of current reagent kits has not been investigated in detail. Here, six commercial kits capable of handling <100ng total RNA input were used for library preparation, performed by kit manufactures, on synthetic miRNAs of known quantities and human total RNA samples. We compared the performance of miRNA detection sensitivity, reliability, titration response and the ability to detect differentially expressed miRNAs. In addition, we assessed the use of unique molecular identifiers (UMI) sequence tags in one kit. We observed differences in detection sensitivity and ability to identify differentially expressed miRNAs between the kits, but none were able to detect the full repertoire of synthetic miRNAs. The reliability within the replicates of all kits was good, while larger differences were observed between the kits, although none could accurately quantify the relative levels of the majority of miRNAs. UMI tags, at least within the input ranges tested, offered little advantage to improve data utility. In conclusion, biases in miRNA abundance are heavily influenced by the kit used for library preparation, suggesting that comparisons of datasets prepared by different procedures should be made with caution. This article is intended to assist researchers select the most appropriate kit for their experimental conditions.