Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2016
Sammendrag
Microsatellite markers are one of the most valuable genetic marker because of high polymorphism, codominant, high reproducibility and relatively high abundance in the genome. Classical techniques to identify and to develop microsatellite markers are time-consuming and require cloning and library construction followed by Sanger sequencing. In the recent years Next Generation Sequencing (NGS) have been widely used to identify molecular markers for non-model organisms. To test the efficiency of NGS techniques in developing molecular markers, we have used double digest Restriction site Associated DNA Sequencing (ddRADseq) to identify microsatellites in Heracleum. Genomic DNA from three individuals digested with SbfI and NdeI followed by size selection and library construction and then DNA fragments were sequenced with Ion Torrent PGM. After trimming adaptors and evaluating the quality of reads, QDD software was used to screen reads with microsatellite motives containing two, three, four, five and six nucleotide repeats. Almost 2% of all sequences were consisted microsatellites repeats. Fifty four singleton and consensus sequences were bioanformatically confirmed and were checked for contamination and similarity with NCBI nucleotide database. Seventy percent of the sequences were represented by (AT)n, (AT)n, (GA)n and (AC)n motives. Twenty five primer pairs were selected to test for amplification and the results showed that most of the loci produced the expected size on Agarose gel. Our results show the high efficiency of ddRADseq in developing sufficient number of markers in a short time where the budget is also limited. Keywords: Microsatellites, Next Generation Sequencing, ddRADseq, Heracleum
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Mari Mette TollefsrudSammendrag
Det er ikke registrert sammendrag
Forfattere
Mari Mette TollefsrudSammendrag
Det er ikke registrert sammendrag
Forfattere
Mari Mette TollefsrudSammendrag
Det er ikke registrert sammendrag
Forfattere
Yoshiaki Tsuda Jun Chen Michael Stocks Thomas Källman Jørn Henrik Sønstebø Laura Parducci Vladimir Semerikov Christoph Sperisen Dmitry Politov Tiina Ronkainen Minna Väliranta Giovanni Giuseppe Vendramin Mari Mette Tollefsrud Martin LascouxSammendrag
Boreal species were repeatedly exposed to ice ages and went through cycles of contraction and expansion while sister species alternated periods of contact and isolation. The resulting genetic structure is consequently complex, and demographic inferences are intrinsically challenging. The range of Norway spruce (Picea abies) and Siberian spruce (Picea obovata) covers most of northern Eurasia; yet their geographical limits and histories remain poorly understood. To delineate the hybrid zone between the two species and reconstruct their joint demographic history, we analysed variation at nuclear SSR and mitochondrial DNA in 102 and 88 populations, respectively. The dynamics of the hybrid zone was analysed with approximate Bayesian computation (ABC) followed by posterior predictive structure plot reconstruction and the presence of barriers across the range tested with estimated effective migration surfaces. To estimate the divergence time between the two species, nuclear sequences from two well-separated populations of each species were analysed with ABC. Two main barriers divide the range of the two species: one corresponds to the hybrid zone between them, and the other separates the southern and northern domains of Norway spruce. The hybrid zone is centred on the Urals, but the genetic impact of Siberian spruce extends further west. The joint distribution of mitochondrial and nuclear variation indicates an introgression of mitochondrial DNA from Norway spruce into Siberian spruce. Overall, our data reveal a demographic history where the two species interacted frequently and where migrants originating from the Urals and the West Siberian Plain recolonized northern Russia and Scandinavia using scattered refugial populations of Norway spruce as stepping stones towards the west.
Forfattere
Mari Mette TollefsrudSammendrag
Det er ikke registrert sammendrag
Forfattere
Tor MykingSammendrag
Det er ikke registrert sammendrag
Forfattere
Tor MykingSammendrag
Det er ikke registrert sammendrag