Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2011
Sammendrag
Wood exhibits a highly anisotropic mechanical behavior due to its heterogeneous microscopic structure and composition. Its microstructure is organized in a strictly hierarchical manner from a length scale of some nanometers, where the elementary constituents cellulose, hemicelluloses, lignin, and extractives are found, up to a length scale of some millimeters, where growth rings composed of earlywood and latewood are observed. To resolve the microscale origin of the mechanical response of the macro-homogeneous but micro-heterogeneous material wood, micromechanical modeling techniques were applied. They allow for prediction of clear wood stiffness (Hofstetter et al. 2005,2007, Bader et al. 2010a,b) from microstructural characteristics. Fungal decay causes changes in the wood microstructure, expressed by decomposition or degradation of its components (Côté 1965, Schwarze 2007). Consequently, macroscopic mechanical properties are decreasing (see e.g. Wilcox 1978). Thus, in the same manner as for clear wood, consideration of alterations of wood in a micromechanical model allows predicting changes in the macroscopic mechanical properties. This contribution covers results from an extensive experimental program, where changes in chemophysical properties and corresponding changes in the mechanical behavior were investigated. For this purpose, pine (Pinus sylvestris) sapwood samples were measured in the reference condition, as well as degraded by brown rot (G. loeophyllum trabeum) or white rot (Trametes. versicolor). Stiffness properties of the unaffected and the degraded material were not only measured in uniaxial tension tests in the longitudinal direction, but also in the three principal material directions by means of ultrasonic testing. The experiments revealed transversal stiffness properties to be much more sensitive to degradation than longitudinal stiffness properties. This is due to the degradation of the polymer matrix between the cellulose fibers, which has a strong effect on the transversal stiffness. On the contrary, longitudinal stiffness is mainly governed by cellulose, which is more stable with respect to degradation by fungi. Consequently, transversal stiffness properties or ratios of normal stiffness tensor components may constitute suitable durability indicators. Subsequently, simple micromechanical models, as well as a multiscale micromechanical model for wood stiffness, were applied for verification of hypotheses on degradation mechanisms and model validation.
Sammendrag
In 2005 an extensive test program including field tests was set up in order to obtain more data on the durability and long term performance of modified wood and semi-durable wood species. One of the main challenges for modified wood is to predict accurate service life time in UC3 (Use use class 3, above ground) and UC4 (use class 4, in soil or fresh water contact). So far, data from in-service conditions are rare, while several studies have evaluated the durability in lab or field test exposure. However, there is still a lack of studies comparing replicate modified wood products in both field and lab exposure. This study evaluates the efficacy of modified wood in AWPA E10, three different types of soil in lab (ENV 807), three test fields in-ground (EN 252) and two close to ground (horizontal double layer test) set-ups at two test sites. The test material includes furfurylated, acetylated and thermally modified wood in addition to reference treated and control samples. In laboratory, both furfurylated, acetylated and thermally modified pine (212ºC) performed well. The modified wood samples performed at the same level, or better, than the reference CC and CCA preservatives in retentions for UC4 applications. In the horizontal double layer test, five years is still too short time to be able to draw firm conclusions. However, in the most accelerated HDL set-up, all controls have failed or are moderately to severely decayed whereas most preservative treated, furfurylated and acetylated wood are sound or only slightly decayed. After 5 years of testing CCA-preserved wood performs better in-ground in field tests than in lab tests, whereas modified wood generally performs slightly poorer. Just like in the lab tests, however, acetylated wood performs equal to CCA-preserved wood in UC4. Furfurylated wood performs equal to or better than UC3 level preservative treated wood. Thermally modified wood actually performs much poorer than all preservative treated wood references. Finally, natural durability classification of the same treatment in different lab and field tests was surprisingly similar.
Sammendrag
Scots pine (Pinus sylvestris L.) is an important softwood species in Northern Europe and is frequently used as material for various wood protection systems. In Europe, EN 113 is the standard basidiomycete laboratory durability test method, using mass loss as evaluation criteria. In this paper quantitative real-time PCR (qPCR) and thermogravimetric analysis (TGA) was used to characterize colonization by basidiomycetes in Scots pine sapwood, but also to learn more about the EN 113 test. Two different wood sample sizes were tested. For Gloeophyllum trabeum the largest sample size gave the highest mass loss, while for the smallest samples Trametes versicolor gave the highest mass loss. As expected, fungal DNA content and mass loss in Scots pine sapwood samples decayed by G. trabeum became higher with increasing incubation time of 16 weeks. More unexpectedly, the T. versicolor DNA content in Scots pine sapwood samples was highest at the start of the incubation period and declined during the incubation period, while mass loss increased during the 28 week incubation period. The fungal colonization in the side and middle of EN 113 samples was tested. Highest DNA contents of G. trabeum were measured in the sides during 16 weeks of incubation. The T. versicolor DNA content was higher or similar in the side compared to the middle of the samples until week 20. For weeks 20 and 22 the DNA content was higher in the middle than in the sides, while for the remaining incubation period (weeks 24, 26 and 28) it was quite similar. TGA was shown to be a useful and fast method for chemical characterization of brown rot decayed wood, but cannot be used for white rot decayed wood. For T. versicolor moisture and fungal DNA explained most of the variation in mass loss, while for G. trabeum moisture explained most of the variation in mass loss.
Sammendrag
Moisture is often recognised as a key factor regarding the long time performance of wooden products, and one of the main challenges for timber products is to predict accurate service life in use class 3 (not covered above ground) and use class 4 (in soil or fresh water contact). A range of durability classification studies have been performed both in field and laboratory. But for several wood species information regarding the durability in use class 3 is lacking. Also, there is still a lack of studies comparing replicate wood products in different field exposure situations. This study evaluates the natural durability of different North European wood species in two different climates and in two different use classes. The wood species were compared with imported species and two preservative treatments. The overall picture shows a higher decay rating for wood species tested in ground contact compared with the results from the above ground “Double layer tests”. Moreover, the woods tested in Western Norway are more decayed than those tested in Eastern Norway. These findings can be explained by higher decay risk in use class 4 than in use class 3, and higher decay risk in a humid climate (Western Norway) than in a dry climate (Eastern Norway). The results indicate similar ranking of the durability of the wood species regardless of the environment they have been exposed to. The results from a linear regression show that MOE-loss of the mini-stakes after three years describes 70 % of the variation in decay rating of the “Double layer” stakes after six years exposure in Western Norway. This result strongly indicates that MOE-loss can be a prospective tool for rapid field testing of natural durability of wood.
Sammendrag
To understand the defence mechanisms utilized by decay fungi when exposed to different wood protection systems the study of gene expression can give us some answers. When the DNA sequences are known, primers can be designed to detect transcripts of genes with gene products related to basic cellular processes and hyphal growth. The characteristic gene products induced in different fungi by different wood protection systems can be identified. Studies on the expression of fungal genes will give us a better understanding of the fungal degradation of wood and we can optimize wood protection systems. Hence, no single technique will give us the answer to all questions about the decay of wood we need to gather small pieces of the puzzle using different approaches. The aim of the present study was to investigate the effects of acetylation level on the growth of Postia placenta with regard to amount of total DNA and gene expression targeting 7 different genes. This paper presents preliminary results after 4 weeks of incubation. The results presented in this paper are parts of a larger project which reaches over a period of 36 weeks with sampling times after 12, 20, 28 and 36 weeks. We found no mass loss in the acetylated samples after 4 weeks of incubation in a modified soil-block test. The presence of P. placenta DNA and the absence of mass loss could indicate on an inability of the mycelia to establish a wood exploitation phase. Two genes related to carbohydrate metabolism were expressed in a higher amount in P. placenta during growth on untreated wood than during growth on acetylated wood. However, for a third gene, also related to carbohydrate metabolism, the relationship was the opposite. Two genes related to oxidative metabolism were expressed in a higher amount in P. placenta during growth on acetylated wood than during growth on untreated wood and another two genes related to oxidative metabolism showed inconsistent results.
Sammendrag
Brown rot is the most common and destructive type of fungal decay for wood in service. These fungi depolymerize preferentially the structural carbohydrates, cellulose and hemicellulose in the cell wall leaving oxidized lignin behind. Modified wood can provide protection against a variety of wood deteriorating organisms, including decay fungi. However, there is still little known about the mode of function of the different wood modifications concerning the decay resistance. The biochemical mechanisms and gene products induced in brown rot during growth in modified wood are poorly understood. In this paper the data collected from mass loss studies and qPCR and qRT-PCR were used for profiling growth dynamics and gene expression of the brown rot fungus Postia placenta in different wood substrates through different stages of decay. Pinus sylvestris (L.) sapwood was used for the following treatments and modifications: chromated copper arsenate CCA (0.67%), furfurylation (WPG 37), thermal modification (D212) and acetylation (WPG 23). Untreated Pinus sylvestris (L.) sapwood was used as control. Samples were taken at different time intervals from 2 to 26 weeks. The highest mass loss and the highest fungal DNA content were found in the control samples while acetylated wood had the lowest mass loss and fungal DNA content. These results reflect a close relation of mass loss and fungal DNA content, both reflecting the amount of Postia placenta decaying the samples. Generally, expression of the investigated genes was highest in CCA treated wood. In the beginning of the incubation of all treated wood samples, the genes coding for oxidative metabolic activity had higher expression levels than the untreated control. In the end of the incubation most of these genes were less expressed than in the untreated control. The genes used for carbohydrate metabolism and the alcohol oxidase showed a significant decrease after 14 weeks of incubation. At the same time an increase in gene expression of an enzyme putative involved in lignin decomposition was detected.
Sammendrag
Timber constructions are often built in combination with other materials such as concrete. These materials can influence the timber construction. Moist concrete can e.g. lead to development of molds which creates an unhealthy living area for people. Furthermore, moisture in wood buildings can negatively affect the wood material, which can lead to negative biological activity in timber and possible reduction of strength properties of timber constructions. The present paper introduces a new innovative method of timber protection and describes the influence of moisture on wood and concrete. The new environmental friendly system for protection of timber has been tested on wood destroying fungi and termites. It can be shown that wood protection by means of electro osmotic pulsing technology can preserve wood in laboratory trials. The wood moisture content is reduced when the protection system is installed. Trials on protected wood against subterranean termites showed lower wood moisture content after test of protected samples compared to untreated samples. However, termite activity could not be reduced to a larger extend as the termite living surroundings were not included. It could be shown that humidity in pores of concrete in cellar walls is reduced using electro osmotic pulsing. The drying of concrete when combined with timber constructions can additionally help to reduce timber degradation as all protection measures that lead to a drier building are positive for fungi and subterranean termite control.
Sammendrag
Coated wooden claddings in building facades are widely used in the Scandinavian countries, and are often preferred to other materials. Wood is facing increasing competition from other materials that are less labor intensive at the construction site and materials with less demand for maintenance thru service life, and makes further development of wooden claddings essential. Growth of discoloring moulds on exposed coated wooden claddings is mainly of aesthetic concern, and is especially disfiguring for light-colored surfaces. Growth of surface fungi often initiates repeated cleaning and shorter maintenance intervals, which in turn increase the total cost of ownership for wooden claddings. Cost and effort of ownership are often important factors considered when choosing a product, and the traditionally good market situation for wooden claddings is therefore threatened. The development of real-time PCR (polymerase chain reaction) and taxon-specific primers has provided new possibilities for specific detection and quantification of fungi in their natural substrates. In qPCR (quantitative real-time PCR), the accumulation of the PCR product is detected for each amplification cycle. An efficient and reproducible sampling and extraction of DNA is required for a high-throughput qPCR based quantification of discoloring fungi. The authors have now adjusted DNA isolation protocols and optimized real-time PCR assays for species specific detection of fungi frequently found on painted surfaces (Aureobasidium pullulans, Alternaria alternata, Cladosporium cladosporides, Ulocladium atrum).
Sammendrag
The aim of this study is to see if the raw material influences fixation and leachability of wood preservatives. Moving towards more eco-friendly and –concious society, the wood industries must adjust itself to new rules and regulations. Greener solutions in wood protection are being tested and introduced, older systems are being improved, but questions still arise concerning some elements currently used in wood preservation. Preservatives leaching into the nature is a problem, especially agents that consist copper. Studies have been conducted on the raw material`s influence on impregnability and variations in sapwood penetration have been found. This gives reason to believe that the leaching of wood preservatives is also influenced by the raw material. This study tries to set the variation of leachability of Wolmanit CX-8 and Tanalith into a system, evaluating the origin of a tree and the origin of a sample. The study uses Scots pine (Pinus sylvestris) as a raw material. Material was harvested from different stands in Norway and Denmark. Pine`s sapwood was cut into samples in size of 20x20x50mm. The samples were treated with the wood protection agents Wolmanit CX-8 and Tanalith. The variation in leachability within trees, between trees and between different stands was studied. Within this material, it is possible to trace the individual sample to its original position in the stem. Samples were climatized, impregnated with preservatives and leached according to standard EN84. Copper and boron content in water samples was determined by an ICP (Inductively Coupled Plasma) technique. Comparing leaching results with different variables, correlation was found with latitude and vertical position of the sample, indicating that southern stands leach out more preservative. The lowest part of the tree does not fixate preservatives.
Sammendrag
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