Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2015
Sammendrag
Leaf blotch diseases in wheat can cause yield losses above 30 %. The necrotrophic fungus Parastagonospora nodorum is the dominating leaf blotch pathogen in Norwegian spring wheat. It has been well documented at the seedling stage that the pathogen produces necrotrophic effectors (NEs) which induces cell death in plants carrying susceptibility genes (Snn), allowing the necrotroph to enter. However, the role of these interactions under field conditions is less researched. In this study, we conducted field experiments with bi-parental and association mapping populations of spring wheat, to investigate the role of NE/Snn in adult plant resistance. The populations have been genotyped with the Illumina 90 K SNP chip, P. nodorum has high genetic diversity and both sexual and asexual reproduction, but the actual adaptation of the pathogen population to cultivars with different levels of resistance is not well studied. We are screening a collection of Norwegian isolates from known host sources to look for differences in NE-frequencies and haplotype distribution. The mapping populations are also inoculated and infiltrated with culture filtrates from single isolates on the seedling stage. Isolates involved in novel interactions will be deepsequenced in order to look for candidate effector genes. Potential effector proteins will be purified by LPC and HPLC to confirm their role in disease development.
Sammendrag
Leaf blotch diseases in wheat can cause yield losses above 30 %. The necrotrophic fungus Parastagonospora nodorum is the dominating leaf blotch pathogen in Norwegian spring wheat. It has been well documented at the seedling stage that the pathogen produces necrotrophic effectors (NEs) which induces cell death in plants carrying susceptibility genes (Snn), allowing the necrotroph to enter. However, the role of these interactions under field conditions is less researched. In this study, we conducted field experiments with bi-parental and association mapping populations of spring wheat, to investigate the role of NE/Snn in adult plant resistance. The populations have been genotyped with the Illumina 90 K SNP chip, P. nodorum has high genetic diversity and both sexual and asexual reproduction, but the actual adaptation of the pathogen population to cultivars with different levels of resistance is not well studied. We are screening a collection of Norwegian isolates from known host sources to look for differences in NE-frequencies and haplotype distribution. The mapping populations are also inoculated and infiltrated with culture filtrates from single isolates on the seedling stage. Isolates involved in novel interactions will be deepsequenced in order to look for candidate effector genes. Potential effector proteins will be purified by LPC and HPLC to confirm their role in disease development.
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Matias Pasquali Marco Beyer Antonio Logrieco Kris Audenaert Virgilio Balmas Ryan Basler Anne-Laure Boutigny Jana Chrpova Elżbieta Czembor Tatiana Gagkaeva María Teresa González-Jaén Ingerd Skow Hofgaard Nagehan Desen Köycü Jelena Lević Patricia Marín García Thomas Miedaner Quirico Migheli Antonio Moretti Marina Elsa Herta Müller Françoise Munaut Päivi Parikka Marine Pallez Jonathan Scauflaire Barbara Scherm Slavica Stanković Ulf Thrane Silvio Uhlig Adriaan Vanheule Tapani Yli-Mattila Susanne VogelgsangSammendrag
Det er ikke registrert sammendrag
Forfattere
Nicola La Porta Gaurav Sablok Giovanni Emilliani Ari Hietala Alessio Giovannelli Paolo Fontana Emilio Potenza Paolo BaldiSammendrag
Det er ikke registrert sammendrag
Forfattere
Ari HietalaSammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Marco Ferretti Karin Hansen Vicent Calatayud Marta Camino-Serrano Nathalie Cools Bruno de Vos Tiina M. Nieminen Nenad Potocic Pasi Rautio Marcus Schaub Volkmar Timmermann Liisa Ukonmaanaho Peter WaldnerSammendrag
Det er ikke registrert sammendrag
Forfattere
Marco Ferretti Tanja GM Sanders Alexa Michel Vicent Calatayud Nathalie Cools Elena Gottardini Matthias Haeni Karin Hansen Nenad Potocic Marcus Schaub Volkmar Timmermann Serina Trotzer Elena VanguelovaSammendrag
Det er ikke registrert sammendrag