Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2015
Sammendrag
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Sammendrag
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Sammendrag
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Sammendrag
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Sammendrag
Net blotch is a major barley disease in Norway caused by the necrotrophic fungus Drechslera teres leading to yield losses of up to 40%. At present, resistance of Norwegian cultivars is insufficient. The pathogen secretes necrotrophic effectors (NEs) which act as virulence factors in order to gain entry into and nutrients from the host (Liu et al., 2014). NEs cause a hypersensitive response in the presence of corresponding dominant host susceptibility factors. In this study we examine the potential role of NEs and host receptors in explaining susceptibility to net blotch in Norwegian barley. This knowledge together with an understanding of the genetic background of the Norwegian net blotch population will be utilized to speed up resistance breeding. 365 Norwegian D. teres isolates collected from various regions and years, together with a selection of globally collected isolates, will be RADtag genotyped in order to obtain GBS markers to study the genetic diversity, genomic evolution and population structure of the current Norwegian fungal population and to compare it to pathotypes from other countries. Additionally, this data will allow us to perform Genomewide Association Studies (GWAS) to identify potential novel NE genes. Selected isolates and their culture filtrates will be screened for specific reactions against an association mapping panel of ca. 200 mostly Norwegian barley lines and a biparental mapping population (both genotyped with the Illumina barley 9K chip) to characterize novel NE-host susceptibility interactions and to map the corresponding sensitivity loci. Effector protein candidates will be purified and further analysed to verify their effect on disease development.
Sammendrag
Net blotch is a major barley disease in Norway caused by the necrotrophic fungus Drechslera teres leading to yield losses of up to 40%. At present, resistance of Norwegian cultivars is insufficient. The pathogen secretes necrotrophic effectors (NEs) which act as virulence factors in order to gain entry into and nutrients from the host (Liu et al., 2014). NEs cause a hypersensitive response in the presence of corresponding dominant host susceptibility factors. In this study we examine the potential role of NEs and host receptors in explaining susceptibility to net blotch in Norwegian barley. This knowledge together with an understanding of the genetic background of the Norwegian net blotch population will be utilized to speed up resistance breeding. 365 Norwegian D. teres isolates collected from various regions and years, together with a selection of globally collected isolates, will be RADtag genotyped in order to obtain GBS markers to study the genetic diversity, genomic evolution and population structure of the current Norwegian fungal population and to compare it to pathotypes from other countries. Additionally, this data will allow us to perform Genomewide Association Studies (GWAS) to identify potential novel NE genes. Selected isolates and their culture filtrates will be screened for specific reactions against an association mapping panel of ca. 200 mostly Norwegian barley lines and a biparental mapping population (both genotyped with the Illumina barley 9K chip) to characterize novel NE-host susceptibility interactions and to map the corresponding sensitivity loci. Effector protein candidates will be purified and further analysed to verify their effect on disease development.
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Andrea FickeSammendrag
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Sammendrag
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Forfattere
Andrea FickeSammendrag
Det er ikke registrert sammendrag