Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2016
Forfattere
Johanna Gottschamel Andreas G. Lössl Stephanie Ruf Yanliang Wang Morten Skaugen Ralph Bock Jihong Liu ClarkeSammendrag
Abstract Dengue fever is a disease in many parts of the tropics and subtropics and about half the world’s popula- tion is at risk of infection according to the World Health Organization. Dengue is caused by any of the four related dengue virus serotypes DEN-1, -2, -3 and -4, which are transmitted to people by Aedes aegypti mosquitoes. Cur- rently there is only one vaccine (DengvaxiaÒ) available (limited to a few countries) on the market since 2015 after half a century’s intensive efforts. Affordable and accessible vaccines against dengue are hence still urgently needed. The dengue envelop protein domain III (EDIII), which is capable of eliciting serotype-specific neutralizing antibod- ies, has become the focus for subunit vaccine development. To contribute to the development of an accessible and affordable dengue vaccine, in the current study we have used plant-based vaccine production systems to generate a dengue subunit vaccine candidate in tobacco. Chloroplast genome engineering was applied to express serotype- specific recombinant EDIII proteins in tobacco chloroplasts using both constitutive and ethanol-inducible expression systems. Expression of a tetravalent antigen fusion con- struct combining EDIII polypeptides from all four ser- otypes was also attempted. Transplastomic EDIII- expressing tobacco lines were obtained and homoplasmy was verified by Southern blot analysis. Northern blot analyses showed expression of EDIII antigen-encoding genes. EDIII protein accumulation levels varied for the different recombinant EDIII proteins and the different expression systems, and reached between 0.8 and 1.6 % of total cellular protein. Our study demonstrates the suitability of the chloroplast compartment as a production site for an EDIII-based vaccine candidate against dengue fever and presents a GatewayÒ plastid transformation vector for inducible transgene expression.
Forfattere
Xurong Mei Hongmin Dong Yue Li Nicholas Clarke Daozhi Gong Weiping Hao Yingchun Li Buchun Liu Xin Ma Wei XiongSammendrag
Det er ikke registrert sammendrag
Forfattere
Mei Xurong Dong Hongmin Li Yue Nicholas Clarke Gong Daozhi Hao Weiping Li Yingchun Liu Buchun Ma Xin Xiong WeiSammendrag
Det er ikke registrert sammendrag
Forfattere
Mei Xurong Dong Hongmin Li Yue Nicholas Clarke Gong Daozhi Hao Weiping Li Yingchun Liu Buchun Ma Xin Xiong WeiSammendrag
Det er ikke registrert sammendrag
Forfattere
Nicholas ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Nicholas ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Nicholas ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Nicholas ClarkeSammendrag
Det er ikke registrert sammendrag
Forfattere
Nathalie Cools Arne Verstraeten Wendelin Weis Stefano Carnicelli Guia Cecchini Nicholas Clarke Elisabeth Graf Pannatier Jim Johnson Mathieu Jonard Henning Meesenburg Tiina M. Nieminen Gunilla Pihl Karlsson Elena Vanguelova Jörg Sintermann Lars Vesterdal Karin HansenSammendrag
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Sammendrag
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