Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2019
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Volkmar Timmermann Nicholas Clarke Jane Uhd Jepsen Jørn-Frode Nordbakken Bjørn Økland Tonje Økland Wenche AasSammendrag
Det er ikke registrert sammendrag
Forfattere
Andre van Eerde Aniko Varnai John-Kristian Jameson Lisa Paruch Anders Moen Jan Haug Anonsen Piotr Chylenski Hege Særvold Steen Inger Heldal Ralph Bock Vincent Eijsink Jihong Liu ClarkeSammendrag
Sustainable production of biofuels from lignocellulose feedstocks depends on cheap enzymes for degradation of such biomass. Plants offer a safe and cost‐effective production platform for biopharmaceuticals, vaccines and industrial enzymes boosting biomass conversion to biofuels. Production of intact and functional protein is a prerequisite for large‐scale protein production, and extensive host‐specific post‐translational modifications (PTMs) often affect the catalytic properties and stability of recombinant enzymes. Here we investigated the impact of plant PTMs on enzyme performance and stability of the major cellobiohydrolase TrCel7A from Trichoderma reesei, an industrially relevant enzyme. TrCel7A was produced in Nicotiana benthamiana using a vacuum‐based transient expression technology, and this recombinant enzyme (TrCel7Arec) was compared with the native fungal enzyme (TrCel7Anat) in terms of PTMs and catalytic activity on commercial and industrial substrates. We show that the N‐terminal glutamate of TrCel7Arec was correctly processed by N. benthamiana to a pyroglutamate, critical for protein structure, while the linker region of TrCel7Arec was vulnerable to proteolytic digestion during protein production due to the absence of O‐mannosylation in the plant host as compared with the native protein. In general, the purified full‐length TrCel7Arec had 25% lower catalytic activity than TrCel7Anat and impaired substrate‐binding properties, which can be attributed to larger N‐glycans and lack of O‐glycans in TrCel7Arec. All in all, our study reveals that the glycosylation machinery of N. benthamiana needs tailoring to optimize the production of efficient cellulases.
Forfattere
Volkmar Timmermann Kjell Andreassen May Bente Brurberg Isabella Børja Nicholas Clarke Daniel Flø Jane Uhd Jepsen Torstein Kvamme Jørn-Frode Nordbakken Per Holm Nygaard Martin Pettersson Sverre Solberg Halvor Solheim Venche Talgø Ole Petter Laksforsmo Vindstad Gro Wollebæk Bjørn Økland Wenche AasSammendrag
Skogens helsetilstand påvirkes i stor grad av klima og værforhold, enten direkte ved tørke, frost og vind, eller indirekte ved at klimaet påvirker omfanget av soppsykdommer og insektangrep. Klimaendringene og den forventede økningen i klimarelaterte skogskader gir store utfordringer for forvaltningen av framtidas skogressurser. Det samme gjør invaderende skadegjørere, både allerede etablerte arter og nye som kan komme til Norge i nær framtid. I denne rapporten presenteres resultater fra skogskadeovervåkingen i Norge i 2018 og trender over tid for følgende temaer: (i) Landsrepresentativ skogovervåking; (ii) Skogøkologiske analyser og målinger av luftkjemi på de intensive overvåkingsflatene; (iii) Overvåking av bjørkemålere i Troms og Finnmark; (iv) Granbarkbilleovervåking – utvikling av barkbillepopulasjonene i 2018; (v) Ny barkbille på vei – vil den like klimaet?; (vi) Phytophthora i importert jord på prydplanter og faren det utgjør for skog; (vii) Overvåking av askeskuddsyke; (viii) Skog- og utmarksbranner i 2018; (ix) Andre spesielle skogskader i 2018...….
2018
Forfattere
Adam O'Toole Christophe Moni Simon Weldon Anne Schols Monique Carnol Bernard Bosman Daniel RasseSammendrag
The application of biochar to soils is a promising technique for increasing soil organic C and offsetting GHG emissions. However, large-scale adoption by farmers will likely require the proof of its utility to improve plant growth and soil quality. In this context, we conducted a four-year field experiment between October 2010 to October 2014 on a fertile silty clay loam Albeluvisol in Norway to assess the impact of biochar on soil physical properties, soil microbial biomass, and oat and barley yield. The following treatments were included: Control (soil), miscanthus biochar 8 t C ha1 (BC8), miscanthus straw feedstock 8 t C ha1 (MC8), and miscanthus biochar 25 t C ha1 (BC25). Average volumetric water content at field capacity was significantly higher in BC25 when compared to the control due to changes in BD and total porosity. The biochar amendment had no effect on soil aggregate (2–6 mm) stability, pore size distribution, penetration resistance, soil microbial biomass C and N, and basal respiration. Biochar did not alter crop yields of oat and barley during the four growing seasons. In order to realize biochar’s climate mitigation potential, we suggest future research and development efforts should focus on improving the agronomic utility of biochar in engineered fertilizer and soil amendment products.
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Frans-Jan W. Parmentier Daniel Rasse Magnus Lund Jarle W. Bjerke Bert G. Drake Simon Weldon Hans Tømmervik Georg Heinrich HansenSammendrag
Extreme winter events that damage vegetation are considered an important climatic cause of arctic browning—a reversal of the greening trend of the region—and possibly reduce the carbon uptake of northern ecosystems. Confirmation of a reduction in CO2 uptake due to winter damage, however, remains elusive due to a lack of flux measurements from affected ecosystems. In this study, we report eddy covariance fluxes of CO2 from a peatland in northern Norway and show that vegetation CO2 uptake was delayed and reduced in the summer of 2014 following an extreme winter event earlier that year. Strong frost in the absence of a protective snow cover—its combined intensity unprecedented in the local climate record—caused severe dieback of the dwarf shrub species Calluna vulgaris and Empetrum nigrum. Similar vegetation damage was reported at the time along ~1000 km of coastal Norway, showing the widespread impact of this event. Our results indicate that gross primary production (GPP) exhibited a delayed response to temperature following snowmelt. From snowmelt up to the peak of summer, this reduced carbon uptake by 14 (0–24) g C m−2 (~12% of GPP in that period)—similar to the effect of interannual variations in summer weather. Concurrently, remotely-sensed NDVI dropped to the lowest level in more than a decade. However, bulk photosynthesis was eventually stimulated by the warm and sunny summer, raising total GPP. Species other than the vulnerable shrubs were probably resilient to the extreme winter event. The warm summer also increased ecosystem respiration, which limited net carbon uptake. This study shows that damage from a single extreme winter event can have an ecosystem-wide impact on CO2 uptake, and highlights the importance of including winter-induced shrub damage in terrestrial ecosystem models to accurately predict trends in vegetation productivity and carbon sequestration in the Arctic and sub-Arctic.
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Norway is strongly committed to the Paris Climate Agreement with an ambitious goal of 40% reduction in greenhouse gas emission by 2030. The land sector, including agriculture and forestry, must critically contribute to this national target. Beyond emission reduction, the land sector has the unique capacity to actively removing CO2 from the atmosphere through biological carbon storage in biomass and in soils. Soils are the largest reservoir of terrestrial carbon, and relatively small changes in soil carbon content can have an amplified mitigation effect on the Earth’s climate. Therefore, improved management of soils for carbon storage is receiving a lot of attention, for example through international political initiatives such as the “4-permill” initiative. However, in Norway, many mitigation measures targeting soil carbon might negatively impact food production and economic activity. For example, soil carbon storage can be increased by shifting from cereal crop production to grasslands, but Norway already has abundant grassland and a comparatively small area dedicated to cereals. Another such issue is cultivation on drained peatland, where food is produced at the expense of large losses of soil carbon as CO2 to the atmosphere. Therefore, there is a need to look for win-win solutions for soil carbon storage, which benefit both food production and climate mitigation. Large-scale conversion of agricultural and forest waste biomass to biochar is such an option, and is considered the activity with the largest potential for soil carbon sequestration in Norway. Biochar has been demonstrated to have a mean residence time exceeding 100 years in Norwegian field conditions (Rasse et al, 2017), and no negative effects on plant and soils has been observed. However, despite the convincing benefits of biochar as a climate mitigation solution, it has not yet advanced much beyond the research stage, notably because its effect on yield are too modest. Here, we will first present the comparative advantage of biochar technology as compared to traditional agronomy methods for large-scale C storage in Norwegian agricultural soils. We will further discuss the need for developing innovations in pyrolysis and nutrient-rich waste recycling leading to biochar-fertilizer products as win-win solution for carbon storage and food production.
Forfattere
Van Minh Dang Stephen Joseph Huu Tap Van Thi Lan Anh Mai Thi Minh Hoa Duong Simon Weldon Paul Munroe David Mitchell Sarasadat TaherymoosaviSammendrag
Heavy metal contamination of crop lands surrounding mines in North Vietnam is a major environmental issue for both farmers and the population as a whole. Technology for the production of biochar at a village and household level has been successfully introduced into Vietnamese villages. This study was undertaken to determine if rice straw biochar produced in simple drum ovens could remediate contaminated land. Tests were also carried out to determine if biochar and apatite mixed together could be more effective than biochar alone. Incubation trials were carried out over 90 days in pots to determine the total changes in exchangeable Cd, Pb and Zn. Detailed tests were carried out to determine the mechanisms that bound the heavy metals to the biochar. It was found that biochar at 5% (BC5) and the mixture of biochar and apatite at 3% (BCA3) resulted in the greatest reduction of exchangeable forms of Cd, Pb and Zn. The increase in soil pH caused by adding biochar and apatite created more negative charge on the soil surface that promoted Pb, Zn and Cd adsorption. Heavy metals were mainly bound in the organic, Fe/Mn and carbonate fractions of the biochar and the mixture of biochar and apatite by either ion exchange, adsorption, dissolution/precipitation and through substitution of cations in large organic molecules.