Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2022
Abstract
No abstract has been registered
Abstract
No abstract has been registered
Abstract
In a fertiliser experiment in a Norway spruce forest in SE Norway, four treatments were applied in a block design with three replicates per treatment. Treatments included 3 t wood ash ha−1 (Ash), 150 kg nitrogen ha−1 (N), wood ash and nitrogen combined (Ash + N), and unfertilised control (Ctrl). Treatment effects on understory plant species numbers, single abundances of species and (summarised) cover of main species groups were studied. Two years after treatment there were no significant changes for species numbers or abundances of woody species, dwarf shrubs or pteridophytes, nor for Sphagnum spp. in the bottom layer. The cover of graminoids decreased in Ctrl plots. Herb cover increased significantly in Ash + N and N plots due to the increase of Melampyrum sylvaticum. In Ash + N plots, mosses decreased significantly in species number, while their cover increased. Moss cover also decreased significantly in N plots. The species number and cover of hepatics decreased significantly in Ash and Ash + N plots. Hepatics cover also decreased in Ctrl plots. Both the lichen number and cover decreased in Ash + N plots. Single species abundances decreased for many bryophytes in fertilised plots. To conclude, fertilisation had modest effects on vascular plants, while bryophytes were more strongly affected, especially by Ash + N.
Authors
Andre van Eerde Aniko Varnai Yanliang Wang Lisa Paruch John-Kristian Jameson Fen Qiao Hans Geir Eiken Hang Su Vincent Eijsink Jihong Liu ClarkeAbstract
Giant panda could have bamboo as their exclusive diet for about 2 million years because of the contribution of numerous enzymes produced by their gut bacteria, for instance laccases. Laccases are blue multi-copper oxidases that catalyze the oxidation of a broad spectrum of phenolic and aromatic compounds with water as the only byproduct. As a “green enzyme,” laccases have potential in industrial applications, for example, when dealing with degradation of recalcitrant biopolymers, such as lignin. In the current study, a bacterial laccase, Lac51, originating from Pseudomonas putida and identified in the gut microbiome of the giant panda’s gut was transiently expressed in the non-food plant Nicotiana benthamiana and characterized. Our results show that recombinant Lac51 exhibits bacterial laccase properties, with optimal pH and temperature at 7–8 and 40°C, respectively, when using syringaldazine as substrate. Moreover, we demonstrate the functional capability of the plant expressed Lac51 to oxidize lignin using selected lignin monomers that serve as substrates of Lac51. In summary, our study demonstrates the potential of green and non-food plants as a viable enzyme production platform for bacterial laccases. This result enriches our understanding of plant-made enzymes, as, to our knowledge, Lac51 is the first functional recombinant laccase produced in plants.
2021
Authors
Liang Wang Alba Dieguez-Alonso Maria Nicte Polanco Olsen Simon Weldon Alice Budai Daniel Rasse Øyvind SkreibergAbstract
No abstract has been registered
Abstract
Biochar-based fertilizer products (BCF) are receiving increasing attention as potential win-win solutions for mitigating climate change and improving agricultural production. BCFs are reported to increase yields through increased N use efficiency, an effect which is often assumed to result from the slow-release of adsorbed N forms into the soil. Here, we review the magnitude of this effect, the potential for further improvement and the need to consider other mechanisms in product development. Current high-N commercial BCFs are mostly physical blends of biochar and mineral fertilizer, with little evidence of slow-release effects supported by sorption mechanisms. For such products, the main effect potentially results from root-growth promoting factors and from increases in soil pH and Eh and stimulation of beneficial micro-organisms in the rhizosphere, which all result in an increase in uptake of specific nutrients. Our reanalysis of literature data indicates that the median sorption capacity of untreated biochar for mineral N forms requires applying 200 times more biochar than N fertilizer. This ratio needs reducing by at least an order of magnitude for producing efficient sorption-based BCFs. Activation of biochar with acids and oxidizing agents, as reported in many studies, appears to only marginally increase sorption capacity in absolute values. Fixation of clay and organics within the porous structure of biochar appears a more promising technology, suggesting that macro- and mesoporosity is a key biochar property that deserves greater scrutiny and research towards making efficient sorption-based BCFs. Mechanisms of action and dose responses need to be more systematically studied in order to devise products that combine positive effects and can be used within realistic agronomic management practices. Long-term effects resulting from accumulated annual inputs of BCF also need to be better evaluated in terms of nutrient cycling and the progressive improvement of soil health.
Authors
Alexandre Tisserant Marjorie Morales Otávio Cavalett Adam O'Toole Simon Weldon Daniel Rasse Francesco CherubiniAbstract
Limiting temperature rise below 2 °C requires large deployment of Negative Emission Technologies (NET) to capture and store atmospheric CO2. Compared to other types of NETs, biochar has emerged as a mature option to store carbon in soils while providing several co-benefits and limited trade-offs. Existing life-cycle assessment studies of biochar systems mostly focus on climate impacts from greenhouse gasses (GHGs), while other forcing agents, effects on soil emissions, other impact categories, and the implications of a large-scale national deployment are rarely jointly considered. Here, we consider all these aspects and quantify the environmental impacts of application to agricultural soils of biochar from forest residues available in Norway considering different scenarios (including mixing of biochar with synthetic fertilizers and bio-oil sequestration for long-term storage). All the biochar scenarios deliver negative emissions under a life-cycle perspective, ranging from -1.72 ± 0.45 tonnes CO2-eq. ha−1 yr−1 to -7.18 ± 0.67 tonnes CO2-eq. ha−1 yr−1 (when bio-oil is sequestered). Estimated negative emissions are robust to multiple climate metrics and a large range of uncertainties tested with a Monte-Carlo analysis. Co-benefits exist with crop yields, stratospheric ozone depletion and marine eutrophication, but potential trade-offs occur with tropospheric ozone formation, fine particulate formation, terrestrial acidification and ecotoxicity. At a national level, biochar has the potential to offset between 13% and 40% of the GHG emissions from the Norwegian agricultural sector. Overall, our study shows the importance of integrating emissions from the supply chain with those from agricultural soils to estimate mitigation potentials of biochar in specific regional contexts.
Authors
Liang Wang Alba Dieguez-Alonso Maria Nicte Polanco Olsen Alice Budai Daniel Rasse Øyvind SkreibergAbstract
No abstract has been registered
Authors
Liang Wang Alba Dieguez-Alonso Maria Nicte Polanco Olsen Alice Budai Daniel Rasse Ondřej Mašek Øyvind SkreibergAbstract
No abstract has been registered
Authors
Leonor Rodrigues Julia Fohrafellner Brieuc Hardy Bruno Huyghebaert Jens Leifeld Alberto Sanz Cobeña Alice Budai Andis Lazdiņš Arezoo Taghizadeh Axel Don Bartosz Adamczyk Benjamin Gimeno Benjamin Sanchez Bo Stenberg Claudia Di Bene Corina Carranca Dalia Feiziene Daniel Rasse Daria Seitz Dario Fornara Eduardo Aguilera Elena Rodriguez Eloïse Mason Erich Inselsbacher Gabriela Barančíková Grzegorz Siebielec Heide Spiegel Imants Kukuļs Jacek Niedźwiecki Jan P. Lesschen Karin Kauer Kestutis Armolaitis Lilian OSullivan Lenka Pavlu Maarten De Boever Nils Kauer Peter Kuikman Peter Laszlo Raquel Mano Raimonds Kasparinskis Rok Mihelič Sevinc Madenoglu Sophie Cornu Sophie Zechmeister-Boltenstern Stephan Glatzel Sylvain Pellerin Teresa Gómez de la Bárcena Thalisa Slier Thomas Kätterer Martin A. Bolinder Kerstin Berglund Toth Toth GergelyAbstract
Deliverable 2.3. This synthesis identifies the available knowledge of achievable carbon sequestration in mineral soils and GHGs mitigation in organic soils in agricultural land, including pasture/grassland across Europe. The inventory of past and current studies on carbon sequestration and GHGs mitigation measures in agricultural soils and the methodology used for the assessment were considered from 25 Member states (MS) across Europe. The stocktake shows that availability of datasets concerning soil carbon sequestration (SCS) is variable among Europe. While northern Europe and central Europe is relatively well studied, there is a lack of studies comprising parts of Southern, Southeaster and Western Europe. Further, it can be concluded that at present country based knowledge and engagement is still poor; very few countries have an idea on their national-wide achievable carbon sequestration potential. The presented national SCS potentials (MS n=13) do however point towards important contributions to mitigate climate change by covering considerable shares of national greenhouse gas emissions from the agricultural sector in the range of 0.1-27 %, underpinning the importance of further investigations. In contrast to mineral soils, effective mitigation measures for organic soils while maintaining industrial agricultural production are still in its infancy. Very few mitigation options exist to mitigate GHG emissions without compromising agricultural production. Most GHG mitigation practices reported by the MS involve the restoration of organic soils, which means a complete abandonment of land from any agricultural use. Only one contribution (NL) reports possible mitigation potentials, which are based on specific water management measures (water level fixation). Nevertheless, there is an increasing awareness of the need of mitigation measures reflected by the several ongoing research projects on peatland management.