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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2022

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Host-specialist parasites of endangered large vertebrates are in many cases more endangered than their hosts. In particular, low host population densities and reduced among-host transmission rates are expected to lead to inbreeding within parasite infrapopulations living on single host individuals. Furthermore, spatial population structures of directly-transmitted parasites should be concordant with those of their hosts. Using population genomic approaches, we investigated inbreeding and population structure in a host-specialist seal louse (Echinophthirius horridus) infesting the Saimaa ringed seal (Phoca hispida saimensis), which is endemic to Lake Saimaa in Finland, and is one of the most endangered pinnipeds in the world. We conducted genome resequencing of pairs of lice collected from 18 individual Saimaa ringed seals throughout the Lake Saimaa complex. Our analyses showed high genetic similarity and inbreeding between lice inhabiting the same individual seal host, indicating low among-host transmission rates. Across the lake, genetic differentiation among individual lice was correlated with their geographic distance, and assignment analyses revealed a marked break in the genetic variation of the lice in the middle of the lake, indicating substantial population structure. These findings indicate that movements of Saimaa ringed seals across the main breeding areas of the fragmented Lake Saimaa complex may in fact be more restricted than suggested by previous population-genetic analyses of the seals themselves.

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Interactions among fungi and insects involve hundreds of thousands of species. While insect communities on plants have formed some of the classic model systems in ecology, fungus-based communities and the forces structuring them remain poorly studied by comparison. We characterize the arthropod communities associated with fruiting bodies of eight mycorrhizal basidiomycete fungus species from three different orders along a 1200-km latitudinal gradient in northern Europe. We hypothesized that, matching the pattern seen for most insect taxa on plants, we would observe a general decrease in fungal-associated species with latitude. Against this backdrop, we expected local communities to be structured by host identity and phylogeny, with more closely related fungal species sharing more similar communities of associated organisms. As a more unique dimension added by the ephemeral nature of fungal fruiting bodies, we expected further imprints generated by successional change, with younger fruiting bodies harboring communities different from older ones. Using DNA metabarcoding to identify arthropod communities from fungal fruiting bodies, we found that latitude left a clear imprint on fungus-associated arthropod community composition, with host phylogeny and decay stage of fruiting bodies leaving lesser but still-detectable effects. The main latitudinal imprint was on a high arthropod species turnover, with no detectable pattern in overall species richness. Overall, these findings paint a new picture of the drivers of fungus-associated arthropod communities, suggesting that latitude will not affect how many arthropod species inhabit a fruiting body but, rather, what species will occur in it and at what relative abundances (as measured by sequence read counts). These patterns upset simplistic predictions regarding latitudinal gradients in species richness and in the strength of biotic interactions.

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Proper treatment of polyvinyl chloride (PVC) waste is challenge as it is not easily degraded and incineration can lead to environmental issue as it will produce toxic chemicals. In this study, a hydrothermal carbonization approach was applied to treat PVC waste. The influence of exogenous additives on dechlorination efficiency of PVC were evaluated. The results showed that, with exogenous additive, substitution, elimination, dehydration and aromatization reaction were enhanced during hydrothermal carbonization. The maximum dechlorination efficiency of 97.50% was achieved with the mass ratio of 1.4% between rice straw and PVC resin at hydrothermal carbonization temperature 240℃ for 120min. The calorific value of hydrothermal charcoal was relatively higher (39.57MJ/kg ± 0.40MJ/kg), indicating a good combustion process. This study presented a novel and sustainable approach, which could convert PVC-waste as a form of solid fuel.

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Anaerobic digestion of animal slurry to produce biogas is the dominated treatment approach and a storage period is normally applied prior to digestion. Pre-storage, however, contributes to CH4 emissions and results in loss of biogas potential. Manure management was found to be an efficient approach to reduce not only the on-site CH4 emission but may also have extended influence on CH4 emission/losses for storage and subsequent biogas process, while the connection remains unclear. The objective of this study was therefore to evaluate the impact of slurry management (e.g. removal frequency) on CH4 emission (both on-site and storage process prior to biogas) and biogas yield. An experimental pig house for growing-finishing pigs (30–110 kg) and the relevant CH4 emission was monitored for one year. In addition, the specific CH4 activity (SMA) test was conducted and used as an alternative indicator to reflect the impact. Results showed that the manure management affected both on-site and subsequent methane emission; with increased manure removal frequencies, the methane emission became less dependent on variation of temperatures and the specific methanogenesis activity was significantly lower. The highest SMA (100 mL CH4 gVS-1), for instance, was observed from the slurries with limited emptied times, which was 10 times of that from the slurries being emptied three times a week. These findings could enlighten the development of environmentally friendly strategies for animal slurry management and biogas production.

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Due to the diversity of microbiota and the high complexity of their interactions that mediate biogas production, a detailed understanding of the microbiota is essential for the overall stability and performance of the anaerobic digestion (AD) process. This study evaluated the microbial taxonomy, metabolism, function, and genetic differences in 14 full-scale biogas reactors and laboratory reactors operating under various conditions in China. This is the first known study of the microbial ecology of AD at food waste (FW) at a regional scale based on multi-omics (16S rRNA gene amplicon sequencing, metagenomics, and proteomics). Temperature significantly affected the bacterial and archaeal community structure (R2 = 0.996, P = 0.001; R2 = 0.846, P < 0.002) and total inorganic carbon(TIC) slightly changed the microbial structure (R2 = 0.532, P = 0.005; R2 = 0.349, P = 0.016). The Wood-Ljungdahl coupled with hydrogenotrophic methanogenic pathways were dominant in the thermophilic reactors, where the acs, metF, cooA, mer, mch and ftr genes were 10.1-, 2.8-, 16.2-, 1.74-, 4.15-, 1.04-folds of the mesophilic reactors (P < 0.01). However, acetoclastic and methylotrophic methanogenesis was the primary pathway in the mesophilic reactors, where the ackA, pta, cdh and mta genes were 2.2-, 3.2-, 14.3-, 1.88-folds of the thermophilic group (P < 0.01). Finally, the Wilcoxon rank-sum test was applied to explain the cause of the temperature affecting AD microbial activities. The findings have deepened the understanding of the effect of temperature on AD microbial ecosystems and are expected to guide the construction and management of full-scale FW biogas plants.