Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2018
Forfattere
Lone RossSammendrag
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Sammendrag
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Sammendrag
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Forfattere
Lone RossSammendrag
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Sammendrag
Effects of climatic factors and material properties on the development of surface mould growth on wooden claddings were investigated in a laboratory experiment. Specimens of aspen (Populus tremula), Siberian larch (Larix Sibirica), American white oak (Querqus alba), Scots pine (Pinus sylvestris), Norway spruce (Picea abies) and thermally modified pine were incubated in eight climatic chambers at specified wetting periods (2 or 4 h per day), relative humidity (58–86%) and temperature conditions (10–27°C). Surface mould growth was assessed weekly for 13 weeks, and the results were evaluated statistically using Generalized Estimating Equations logistic regression models. All tested climatic factors had significant effects on the mould growth, and there were significant differences between the materials. The ranking of the materials varied with temperature and over time. Aspen, pine sapwood and oak were overall most susceptible to mould growth, and thermally modified pine least susceptible. There were significant differences between sapwood and heartwood for pine and spruce. The effect of density was tested on the spruce heartwood material, but was not found to be significant. The results can be used to further develop prediction models for mould growth on wooden claddings.
Sammendrag
This study addresses changes in visual appearance of unpainted wood materials exposed outdoors. Specimens of Norway spruce (Picea abies) Scots pine (Pinus sylvestris), aspen (Populus tremula), acetylated Radiata pine (Pinus radiata) and DMDHEU-modified Scots pine sapwood were exposed facing south in Ås, Norway for 60 weeks. During this period, surface mould growth development and wasp attack were assessed visually. Development in lightness (L*) and the uniformity of the weather grey colour were assessed by image analysis. The mould rating of the tested wood materials developed in varying speed, but all specimens had reached the maximum rating after 42 weeks. Our results indicate that most specimens continued to darken after the specimens had reached maximum mould rating, and that evaluation of L* can provide additional information about the surface mould growth. Furthermore, our results indicate that most materials developed a less uniform appearance than what was initially, except from DMDHEU which obtained a more uniform appearance as a consequence of the weathering. This study also shows that wasp attack can give a lighter appearance of the wood by chewing off the top weathered layer. Different wood substrates were attacked in varying degree. Aspen was the substrate most severely attacked by wasps while the acetylated wood was not attacked at all during the 60 weeks of exposure.
Forfattere
Steven Tabor Lourdes Orjuela David Contreras Gry Alfredsen Jody Jellison Scott Renneckar Barry GoodellSammendrag
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Forfattere
Olav Aaseth Hegnar Dejan Petrovic Bastien Bissaro Gry Alfredsen Aniko Varnai Vincent EijsinkSammendrag
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that perform oxidative cleavage of recalcitrant polysaccharides. We have purified and characterized a recombinant family AA9 LPMO, LPMO9B, from Gloeophyllum trabeum (GtLPMO9B) which is active on both cellulose and xyloglucan. Activity of the enzyme was tested in the presence of three different reductants: ascorbic acid, gallic acid, and 2,3-dihydroxybenzoic acid (2,3-DHBA). Under standard aerobic conditions typically used in LPMO experiments, the first two reductants could drive LPMO catalysis whereas 2,3-DHBA could not. In agreement with the recent discovery that H2O2 can drive LPMO catalysis, we show that gradual addition of H2O2 allowed LPMO activity at very low, substoichiometric (relative to products formed) reductant concentrations. Most importantly, we found that while 2,3-DHBA is not capable of driving the LPMO reaction under standard aerobic conditions, it can do so in the presence of externally added H2O2. At alkaline pH, 2,3-DHBA is able to drive the LPMO reaction without externally added H2O2, and this ability overlaps entirely the endogenous generation of H2O2 by GtLPMO9B-catalyzed oxidation of 2,3-DHBA. These findings support the notion that H2O2 is a cosubstrate of LPMOs and provide insight into how LPMO reactions depend on, and may be controlled by, the choice of pH and reductant.
Forfattere
Gry AlfredsenSammendrag
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