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Publikasjoner

NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.

2009

Til dokument

Sammendrag

Phytophthora ramorum S. Werres, A.W.A.M. de Cook & W.A. Man in‘t Veld is a newly described Phytophthora-species which is considered to be relatively recently introduced to both USA and Europe from an unknown area, or areas, of origin. The pathogen has a wide host range and causes a complexity of disease symptoms generally grouped into three categories: canker, foliage lesion, and dieback. In Europe the pathogen has been reported in 21 countries, Norway included; predominantly on ornamental plants in nurseries, but also outside nurseries in gardens and semi-natural environment, most often on rhododendrons. The Norwegian Food Safety Authority needs a risk assessment of the pest as basis for an evaluation of a future phytosanitary risk management of P. ramorum, including whether the organism should be regulated as a quarantine pest in Norway. On this background the Norwegian Food Safety Authority, in a letter of 22nd August 2008, requested a pest risk assessment of P. ramorum from the Norwegian Scientific Committee for Food Safety (Vitenskapskomiteen for mattrygghet, VKM). The pest risk assessment was adopted by VKM"s Panel on plant health (Panel 9) on a meeting 24th June 2009. VKM"s Panel 9 gives the following main conclusions of the risk assessment: 1) P. ramorum is present but not widely distributed in Norway, and the pest is under official control. The outdoors surveys of P. ramorum in Norway have not been conducted systematically over the whole country, and some uncertainty therefore still remains regarding the current distribution of P. ramorum in the PRA area. 2) The overall probability of entry of P. ramorum into Norway and the overall probability of establishment of P. ramorum in Norway are both rated as high with low levels of uncertainty; 3) In the absence of statutory control the probability for P. ramorum to be spread quickly in the PRA area by trade of host plants for planting is rated as high. The uncertainty of this assessment is low; 4) The part of the PRA area where presence of P. ramorum might result in economically important losses (the endangered area) is assessed to be most of the country of Norway, except where the climate is predicted to be unfavourable for the pest. However, this area must be regarded as a maximum estimate for the endangered area. On the other hand, a narrow and very conservative estimate for the endangered area can be defined based on the geographical distribution of highly susceptible host plants in Norway. This area is gardens and parks with Rhododendron spp., Viburnum spp. and F. sylvatica and areas in the wild into which Rhododendron spp. has spread and woods with F. sylvatica. Woods with F. sylvatica is limited to the county of Vestfold and some small areas in the counties of Aust-Agder and Hordaland; 5) P. ramorum is likely to have moderate economic impact on the nurseries in the PRA area with current phytosanitary measures. Without any such regulations P. ramorum is likely to have major economic impact on the nursery industry of the PRA area. The levels of uncertainties of these assessments are low; 6) With current phytosanitary measures P. ramorum is likely to have moderate economic impact on parks and private gardens in parts of the PRA area. Without any such regulations P. ramorum is likely to have major economic impact in the best climatic zones of the PRA area. The levels of uncertainties of these assessments are low; 7) The impact of P. ramorum in coniferous and mixed forests of the PRA area is likely to be minor. The level of uncertainty of this assessment is medium. The impact of P. ramorum in natural and planted deciduous broadleaf forests of the PRA area is likely to be minor due to the scattered and limited distribution of the most susceptible species. The level of uncertainty of this assessment is medium; 8) The non-commercial and environmental consequences to natural environments in the PRA area are likely to be moderate. The level of uncertainty ...

Sammendrag

Mite damage is often considered a spray induced problem. We monitored 12 Norwegian strawberry fields, 6 organic and 6 conventional fields, by sampling leaves two times per year for two years. Young folded leaflets were visually inspected for eggs and adults of strawberry mite (Phytonemus pallidus) and completely unfolded leaves were used for extraction of mobile stages of two-spotted spider mite (Tetranychys urticae). The spider mites were examined for infection of the mite-pathogenic fungus Neozygites floridana. Predatory mites (Phytoseiidae) were recorded on both leaf types, and the females mounted and identified. We also sampled leaves from selected plants in the boundary vegetation of most fields, to look for sources of T. urticae, phytoseiids and N. floridana, and soil from each field, to study the natural occurrence of entomopathogenic nematodes. We will present the results on abundance and diversity of the organisms investigated, and discuss similarities and differences between organic and conventional fields.

Sammendrag

Det er mange planter ein må unngå eller vera varsam med å bruka i grøntanlegg på grunn av skadegjerarar. Nokre er det forbod mot å planta, så som pile- og bulkemispel, på grunn av pærebrann. Eit anna døme er ask, der det no er restriksjonar på flytting av askeplanter til smittefrie soner på grunn av askeskotsjuke. Dessutan er det mange planteartar og -sortar som er så utsette for skade at ein i mange anlegg bør unngå dei på grunn av redusert prydverdi, til dømes hagtorn som er svært utstatt for hagtornrust dersom det veks vanleg einer i nærleiken. Her har vi også tatt med ein del planteartar som år om anna vert meir eller mindre skadde ved nokre lokalitetar, til dømes barlind og laurbærhegg. Vi meiner ikkje at desse plantene bør ut av bruk, men vi vil berre gjera merksam på problem som kan førekoma.

2008

Sammendrag

Planteparasittære nematoder er små rundormer som skader røttene på korn. Symptomene viser seg som tynn plantebestand, svake planter og mer ugras. I de siste årene viser henvendelser fra produsenter at problemene med nematoder er økende. Prøver med korncystenematoder, rotsårnematoder og stuntnematoder er vanlig. I prøvene hvor resistente sorter for korncystenematoder er dyrket, og skader vises, er ofte rotsårnematoder funnet. Rotsårnematoder er påvist i forbindelse med skader i alle kornarter. I en del av feltene som er undersøkt i Vestfold, Østfold, Agder, Romerike og Buskerud er det vist til dels høge populasjoner av rotsårnematoder.

Sammendrag

Bioforsk gjennomførte i samarbeid med Lier og omegn forsøksring en kartlegging av planteparasittære nematoder i Lier. Dette p.g.a. at det i 2006 ble påvist skader av nematoder i stilkselleri hos en dyrker i Lier. I vekstsesongen 2007 ble det gjort undersøkelser i 37 prøver fra grønnsakfelt fra 7 produsenter, hvor det ble dyrket blomkål, brokkoli, kinakål, isberg salat, stangselleri, og fra felter som var brakk/pløyd/frest. Det ble funnet stuntnematoder (Tylenchorhynchus sp.) i 27 prøver (72,9 %), Kålcystenematoder (Heterodera cruciferae) og cystenematoder (Heterodera spp.) i 25 prøver (67,5 %), rotsårnematoder (Pratylenchus spp.) i 20 prøver (54 %), pin-nematoder (Paratylenchus spp.) i 11 prøver (29,7 %), og spiralnematoder i 3 prøver (8,1 %).

Sammendrag

Abstract Poinsettia (Euphorbia pulcherrima Willd. Ex Klotzsch), is a contemporary symbol of Christmas in most parts of the world. Today, Europe and North America represent the largest volume of production and sales, but demand is growing quickly in the other regions as poinsettia becomes more popular each year. In Norway, poinsettia is one of the most important pot plants, with a yearly production close to 6 million plants. Its ornamental value and innovation potential have laid the foundation for extensive research in Norway and elsewhere. Poinsettia mosaic virus (PnMV) can cause diseases in modern poinsettia cultivars. PnMV is a single-stranded, positive-sense RNA virus that belongs to the family Tymoviridae. Infection of poinsettia plants with PnMV results in mosaic symptoms during parts of the growing season, which in turn decreases the commercial value of this ornamental plant. Thus, growers are interested in the potential benefits of growing PnMV-free poinsettias. PnMV-free poinsettia plants can be obtained by heat treatment or in vitro culture of apical meristems, which are time-consuming and cost-ineffective methods. There is a need for a new and effective alternative approach, like Agrobacterium-mediated transformation, which can overcome these difficulties. Therefore, we have developed an Agrobacterium-mediated transformation approach for poinsettia for the first time. Internode stem explants of poinsettia cv. Millenium were transformed by Agrobacterium tumefaciens, strain LBA 4404, harbouring three hairpin (hp) RNA gene constructs to induce RNA silencing-mediated resistance to Poinsettia mosaic virus (PnMV). Prior to transformation, an efficient somatic embryogenesis system was developed for poinsettia cv. Millenium in which about 75 % of the explants produced somatic embryos. In five experiments utilizing 868 explants, 18 independent transgenic lines were generated. Stable integration of transgenes into the poinsettia nuclear genome was confirmed by PCR and Southern blot analysis. Both single- and multiple-copy transgene integration into the poinsettia genome were detected among transformants. Northern blot analysis confirmed the production of transgene-derived small interfering RNAs (siRNAs). Transgenic lines showing resistance to mechanical inoculation of PnMV were detected by double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA). The Agrobacterium-mediated transformation methodology developed in the current study should facilitate improvement of this ornamental plant with enhanced disease resistance, quality improvement and desirable colour alteration. Because poinsettia is a non-food, non-feed plant and is not propagated through sexual reproduction, this is likely to be more acceptable even in areas where genetically modified crops are currently not cultivated.

Sammendrag

Poinsettia (Euphorbia pulcherrima Willd. Ex Klotzsch), is a contemporary symbol of Christmas in most parts of the world. Today, Europe and North America represent the largest volume of production and sales, but demand is growing quickly in the other regions as poinsettia becomes more popular each year. In Norway, poinsettia is one of the most important pot plants, with a yearly production close to 6 million plants. Its ornamental value and innovation potential have laid the foundation for extensive research in Norway and elsewhere. Poinsettia mosaic virus (PnMV) is a RNA virus that belongs to the family Tymoviridae. Infection of poinsettia plants with PnMV results in mosaic symptoms during parts of the growing season and decrease the commercial value of this ornamental plant. Thus, growers are interested in the potential benefits of growing PnMV-free poinsettias. PnMV-free poinsettia plants can be obtained by heat treatment or in vitro culture of apical meristems, which are time-consuming and cost-ineffective methods. There is thus an urgent need for a new approach, like Agrobacterium-mediated transformation, which can overcome these difficulties. We have therefore developed an Agrobacterium-mediated transformation approach for poinsettia. Transgenic poinsettia plants with improved resistance against PnMV by expressing hairpin RNA constructs which targeted various regions of the virus genome were produced. Mechanical inoculation of PnMV and subsequent enzyme-linked immunosorbent assay (ELISA) confirmed the PnMV resistance. The siRNA analysis has demonstrated gene silencing mediated resistance. The PnMV resistant transgenic poinsettia lines produced are in the process of being commercialized. Because poinsettia is a non-food, non-feed plant and is not propagated through sexual reproduction, this is likely to be more acceptable even in areas where genetically modified crops are currently not cultivated.