Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2023
Forfattere
Margaret Nyaboke Nyang'au Komivi S. Akutse Khamis Fathiya Miriam Karwitha Charimbu Solveig HaukelandSammendrag
Potato Cyst Nematodes (PCN) (Globodera rostochiensis and G. pallida Woll.) are quarantine pests of potato (Solanum tuberosum L.) worldwide capable of causing significant yield loss and difficult to manage with conventional methods. The study explored the diversity of antagonistic fungi associated with PCN obtained from soil samples collected in Nyandarua and Nakuru Counties in Kenya and their effect on PCN egg viability and hatching was also evaluated. Twelve fungal isolates from five genera were isolated and characterized using morphological and molecular techniques. The twelve isolates were Trichoderma asperellum (4), T. hamatum, T. breve, T. atrobruneum, Amanita basiorubra, Setophoma terrestris (2), Penicillium chrysogenum and Clonostachys rosea. The most abundant isolate was Trichoderma spp. with 58 % occurrence. The effect of seven of the isolates on PCN eggs showed that T. breve and P. chrysogenum reduced egg viability by 41 % and 34 %, respectively while T. asperellum and T. breve reduced their hatching by 50 % on average. Trichoderma atrobrunneum, T. hamatum, and A. basiorubra also reduced the PCN egg viability by 27 % on average. These fungal isolates could provide a potential tool for PCN management in potato production systems for improved yields. However, further studies are warranted to validate these findings under greenhouse and field conditions. A more comprehensive bioprospecting survey for PCN associated antagonistic fungi needs to be extended to other potato growing regions to explore further cyst pathogens.
Sammendrag
Det er ikke registrert sammendrag
Forfattere
James Kisaakye Hendrika Fourie Danny Coyne Laura Cortada Fathiya Mbarak Khamis Sevgan Subramanian Shirlyne Masinde Solveig HaukelandSammendrag
The root-burrowing nematode, Radopholus similis, is reputedly the most damaging nematode pest of banana and responsible for major production losses. In this study, the endophytic potential of 13 fungal isolates was assessed for the management of R. similis in East African Highland bananas (‘Ng’ombe’). All isolates successfully colonised tissue-cultured banana roots, with isolates from Trichoderma, Fusarium and Hypocrea producing the highest (⩾49.1%) and Beauveria isolates the lowest (⩽14.4%) colonisation. The fungal endophytes T. asperellum (ICIPE 700) and H. lixii (ICIPE 697) were the most effective in reducing R. similis densities (>81%) relative to the non-inoculated control. However, the combined inoculation of ICIPE 700 and ICIPE 697 led to greater suppression of R. similis (>21%) relative to individual inoculation. Suppression of R. similis following inoculation of banana roots with ICIPE 700 and/or ICIPE 697 was associated with the significant upregulation of the defence-related gene PR-1, the cell signalling gene calmodulin Ca2+ and the cell-wall-strengthening gene β-1,3-glucan synthase. This study demonstrates the potential for nematode management in bananas with fungal endophytes, especially using the isolates ICIPE 700 and ICIPE 697 when combined.
Forfattere
Natthidech Beesa Buncha Chinnasri Anongnuch Sasnarukkit Tida Dethoup Kansiree Jindapunnapat Agnes W. Kiriga Kanan Saikai Solveig Haukeland Danny CoyneSammendrag
The legume cavalcade, Centrosema pascuorum, is used extensively as a cover crop and as a component of conservation agriculture systems. It is also an attractive rotation or cover crop for the management of root-knot nematodes (RKN; Meloidogyne spp.) as it is a non-host. RKN are persistent pests that are well known to be difficult to control. However, the mechanisms governing the non-host status of cavalcade is unknown. The current study established that cavalcade leaves are toxic to RKN as either aqueous extracts or soil amendments. Bioassays conducted using Meloidogyne javanica showed that a 90% concentration of aqueous extract derived from 1-month-old cavalcade leaves (89 mg crude extract ml−1) suppressed nematode hatch (82.9%) and killed infective second-stage juveniles of M. javanica (85.3%). Soil amendments with 1% (w/w) of 1-month-old cavalcade leaves (0.99 mg crude extract g−1 soil) also provided effective control of M. javanica in the glasshouse on okra. One-month-old leaves appeared more effective than 2- or 3-month-old leaves. The soil amendments had no adverse phytotoxic effect on okra seed germination. Our study demonstrates the potential for using cavalcade leaves or extracts to manage RKN. This may be due to the nematicidal activity of the various compounds in the leaves, such as flavonoids, phenols and terpenoids, which should be further assessed.
Forfattere
Hang Su Andre van Eerde Espen Rimstad Ralph Bock Norica Branza-Nichita Igor A. Yakovlev Jihong Liu ClarkeSammendrag
Plants provide not only food and feed, but also herbal medicines and various raw materials for industry. Moreover, plants can be green factories producing high value bioproducts such as biopharmaceuticals and vaccines. Advantages of plant-based production platforms include easy scale-up, cost effectiveness, and high safety as plants are not hosts for human and animal pathogens. Plant cells perform many post-translational modifications that are present in humans and animals and can be essential for biological activity of produced recombinant proteins. Stimulated by progress in plant transformation technologies, substantial efforts have been made in both the public and the private sectors to develop plant-based vaccine production platforms. Recent promising examples include plant-made vaccines against COVID-19 and Ebola. The COVIFENZ® COVID-19 vaccine produced in Nicotiana benthamiana has been approved in Canada, and several plant-made influenza vaccines have undergone clinical trials. In this review, we discuss the status of vaccine production in plants and the state of the art in downstream processing according to good manufacturing practice (GMP). We discuss different production approaches, including stable transgenic plants and transient expression technologies, and review selected applications in the area of human and veterinary vaccines. We also highlight specific challenges associated with viral vaccine production for different target organisms, including lower vertebrates (e.g., farmed fish), and discuss future perspectives for the field.
Forfattere
Simeon Rossmann Erik Lysøe Monica Skogen Håvard Eikemo Marta Janiszewska Mirella Ludwiczewska Sylwester Sobkowiak Jadwiga Śliwka May Bente BrurbergSammendrag
Det er ikke registrert sammendrag
Forfattere
Simeon Rossmann Erik Lysøe Monica Skogen Håvard Eikemo Marta Janiszewska Mirella Ludwiczewska Sylwester Sobkowiak Jadwiga Śliwka May Bente BrurbergSammendrag
Det er ikke registrert sammendrag
Sammendrag
Overvåkingsprogrammet i 2022 omfattet undersøkelse for tilstedeværelse av furuvednematode (Bursaphelenchus xylophilus) i hogstavfall fra furu og i furubukker av slekten Monochamus. I OK-programmets delaktivitet som omfattet kartlegging av furuvednematode i hogstavfall, ble det tatt 401 flisprøver fra hogstavfall og vindfall av furu (Pinus sylvestris) som hadde tegn på angrep av furubukker i slekten Monochamus. Prøvene ble tatt i Innlandet, Vestfold og Telemark, og Viken. Flisprøvene ble inkubert ved +25°C i to uker før nematoder ble ekstrahert med Baermann-trakt og undersøkt i mikroskop. Furuvednematoden B. xylophilus ble ikke påvist, men den naturlig forekommende arten Bursaphelenchus mucronatus kolymensis ble oppdaget i 16 flisprøver. Siden overvåkingen av furuvednematode startet i 2000, har alle de analyserte flisprøvene, totalt 8924, vært negative for furuvednematode. I OK-programmets delaktivitet som omfattet kartlegging av furuvednematode i furubukker, ble feller med attraktanter for fangst av voksne, flygende furubukker satt opp i Agder, Telemark, Viken (Østfold og Akershus), Hedmark og Trøndelag. Billene ble kuttet i biter og ekstrahert med en modifisert Baermann-trakt. Suspensjonen fra ekstraksjonene ble undersøkt i mikroskop for forekomst av Bursaphelenchus spp. Ingen furuvednematoder ble påvist i de 54 undersøkte billene. Bursaphelenchus mucronatus kolymensis ble ikke heller oppdaget.
Sammendrag
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Sammendrag
Det er ikke registrert sammendrag