Publikasjoner
NIBIOs ansatte publiserer flere hundre vitenskapelige artikler og forskningsrapporter hvert år. Her finner du referanser og lenker til publikasjoner og andre forsknings- og formidlingsaktiviteter. Samlingen oppdateres løpende med både nytt og historisk materiale. For mer informasjon om NIBIOs publikasjoner, besøk NIBIOs bibliotek.
2017
Sammendrag
The genus Pectobacterium, which belongs to the bacterial family Enterobacteriaceae, contains numerous species that cause soft rot diseases in a wide range of plants. The species Pectobacterium carotovorum is highly heterogeneous, indicating a need for re-evaluation and a better classification of the species. PacBio was used for sequencing of two soft-rot-causing bacterial strains (NIBIO1006T and NIBIO1392), initially identified as P. carotovorum strains by fatty acid analysis and sequencing of three housekeeping genes (dnaX, icdA and mdh). Their taxonomic relationship to other Pectobacterium species was determined and the distance from any described species within the genus Pectobacterium was less than 94% average nucleotide identity (ANI). Based on ANI, phylogenetic data and genome-to-genome distance, strains NIBIO1006T, NIBIO1392 and NCPPB3395 are suggested to represent a novel species of the genus Pectobacterium, for which the name Pectobacterium polaris sp. nov. is proposed. The type strain is NIBIO1006T (=DSM 105255T=NCPPB 4611T).
Sammendrag
Det er ikke registrert sammendrag
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Nina Johansen Carl Jonas Jorge Spetz Annette Folkedal Schjøll Tor J. Johansen Borghild GlorvigenSammendrag
Det er ikke registrert sammendrag
Forfattere
Jihong Liu Clarke Lisa Paruch Mihaela-Olivia Dobrica Iuliana Caras Catalin Tucureanu Adrian Onu Sonya Ciulean Crina Stavaru Andre van Eerde Hege Særvold Steen Sissel Haugslien Catalina Petrareanu Catalin Lazar Ioan Popescu Ralph Bock Jean Dubuisson Norica Branza-NichitaSammendrag
Det er ikke registrert sammendrag
Forfattere
Jihong Liu Clarke Lisa Paruch Mihaela-Olivia Dobrica Iuliana Caras Catalin Tucureanu Adrian Onu Sonya Ciulean Crina Stavaru Andre van Eerde Yanliang Wang Hege Særvold Steen Sissel Haugslien Catalina Petrareanu Catalin Lazar Costin-loan Popescu Ralph Bock Jean Dubuisson Norica Branza-NichitaSammendrag
The hepatitis C virus (HCV) is a major etiologic agent for severe liver diseases ( e.g . cirrhosis, fibrosis and hepatocellular carcinoma). Approximately 140 million people have chronic HCV infections and about 500 000 die yearly from HCV-related liver pathologies. To date, there is no licensed vaccine available to prevent HCV infection and production of a HCV vaccine remains a major challenge. Here, we report the successful production of the HCV E1E2 heterodimer, an important vaccine candidate, in an edible crop (lettuce, Lactuca s ativa ) using Agrobacterium - mediated transient expression technology. The wild-type dimer (E1E2) and a variant without an N-glycosylation site in the E2 polypeptide (E1E2 Δ N6) were expressed, and appropriate N-glycosylation pattern and functionality of the E1E2 dimers were demonstrated. The humoral immune response induced by the HCV proteins was investigated in mice following oral administration of lettuce antigens with or without previous intramuscular prime with the mammalian HEK293T cell-expressed HCV dimer. Immunization by oral feeding only resulted in development of weak serum levels of anti-HCV IgM for both antigens; however, the E1E2 Δ N6 proteins produced higher amounts of secretory IgA, suggesting improved immunogenic properties of the N-glycosylation mutant. The mice group receiving the intramuscular injection followed by two oral boosts with the lettuce E1E2 dimer developed a systemic but also a mucosal immune response, as demonstrated by the presence of anti-HCV secretory IgA in faeces extracts. In summary, our study demonstrates the feasibility of producing complex viral antigens in lettuce, using plant transient expression technology, with great potential for future low-cost oral vaccine development.
Sammendrag
Det er ikke registrert sammendrag
Forfattere
Leif Sundheim Daniel Flø Micael Wendell Guro Brodal Åshild Ergon Christer Magnusson Arild Sletten Halvor Solheim Anne Marte Tronsmo Bjørn Økland Trond RafossSammendrag
Det er ikke registrert sammendrag
Forfattere
Jihong Liu Clarke Andre van Eerde Lisa Paruch Inger Heldal Hege Særvold Steen Yanliang Wang Astrid Sivertsen Sissel HaugslienSammendrag
Det er ikke registrert sammendrag
Forfattere
Volkmar Timmermann Kjell Andreassen Nicholas Clarke Daniel Flø Christer Magnusson Jørn-Frode Nordbakken Ingvald Røsberg Halvor Solheim Karl Thunes Gro Wollebæk Bjørn Økland Wenche AasSammendrag
Skogens helsetilstand påvirkes i stor grad av klima og værforhold, enten direkte ved tørke, frost og vind, eller indirekte ved at klimaet påvirker omfanget av soppsykdommer og insektangrep. Klimaendringene og den forventede økningen i klimarelaterte skogskader gir store utfordringer for forvaltningen av framtidas skogressurser. Det samme gjør invaderende skadegjørere, både allerede etablerte arter og nye som kan komme til Norge som følge av økt handel og import. Eksempler på begge er omtalt i denne rapporten som presenterer resultater fra skogskadeovervåkingen i Norge i 2016........