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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2026

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Abstract

The predicted increase in frequency and duration of winter warming episodes (WWEs) at higher northern latitudes is expected to negatively impact the forage production in this region. The formation of non-permeable ice cover due to WWEs creates hypoxic or anoxic conditions for plants, leading to severe winter damage. Knowledge about molecular mechanisms underlying various winter stresses, including ice encasement, is crucial to develop cultivars with better winter survival under changing climatic conditions. To date, very little is known about the molecular stress responses under ice encasement stress. To address this knowledge gap, in this study, we aimed to study ice encasement stress responses at the molecular level in the perennial forage grass timothy (Phleum pratense L.) by RNAseq. Genes encoding ethylene-responsive transcription factors, alcohol dehydrogenase 3, pyruvate decarboxylase 2, pyruvate kinase 1, dehydrins, early response to dehydration 15, glutathione reductase, and superoxide dismutase were highly upregulated under ice encasement conditions. KEGG enrichment analysis identified glycolysis, glutathione metabolism, and fructose and mannose metabolism as highly enriched among upregulated genes, whereas photosynthesis, flavonoid biosynthesis, motor proteins, and glycerolipid metabolism were highly enriched among downregulated genes. As initially hypothesized based on the nature of stress, the results indicate a substantial overlap of ice encasement stress responses with those of hypoxia and freezing stresses. Based on our findings and a comprehensive literature review on freezing and hypoxia stress responses, together with physiological studies of plants under ice encasement, we outline the potential mechanisms behind higher ice encasement tolerance in timothy.

Abstract

Fish sludge (faeces and fish residues from farmed fish) is currently not permitted as an ingredient in CE-marked fertilising products under the EU Fertilising Product Regulation No. 2019/2009 (FPR). This report provides the scientific basis for a potential proposal to include fish sludge in the FPR by 1) summarising existing knowledge on contaminant levels and the agronomic efficiency, and 2) identifying knowledge gaps. The legal status of fish sludge under the Animal by-products regulation (EU) No. 1069/2009 (ABP) has to be clarified first but is outside the scope of the report. Most available data on fish sludge originate from land-based Atlantic salmon production in Norway. A major knowledge gap is therefore the lack of data on contaminant levels and the agronomic efficiency of fish sludge from sea-based systems, as well as from other countries and aquaculture species. With respect to contaminant levels in fish sludge from land-based smolt systems in Norway, additional data are required for hexavalent chromium, inorganic arsenic, pharmaceuticals, and certain pesticides. The agronomic efficiency of fish sludge from land-based smolt systems in Norway is relatively well documented.

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Abstract

Increasing numbers of invasive Pacific pink salmon (Oncorhynchus gorbuscha) are spawning in northern Norwegian rivers, leading to large numbers of juveniles migrating to coastal waters. This raises concerns about food competition with native salmonids in rivers, although details about the timing and intensity of pink salmon feeding and the characteristics of their freshwater diet remain unclear. Some evidence indicates that pink salmon can also serve as a food source for native salmonids, as do the carcasses of spawned adults, eggs and juveniles. This study investigated largely unexplored aspects of the emergence timing and feeding behaviour of juvenile pink salmon. The prevalence, timing and impact of fish size on freshwater feeding was examined across 10 rivers in northern Norway, using stomach content and stable isotope (δ13C and δ15N) analyses to assess diet composition. Similar methods were applied to assess whether native juvenile salmonids feed on pink salmon tissue, such as juveniles, during their migration. Predictions of the emergence times of alevins show that later spawning, thought to cause increased potential negative interactions with native fish, is an unlikely strategy for pink salmon in northern Norway. In three of the rivers, some of the largest free-swimming pink salmon juveniles sampled in this study (30–35 mm) began external feeding on freshwater invertebrates later in the season (June and July), with larval and pupae chironomids as well as blackfly and mayfly nymphs dominating their diets. Feeding was not observed in all rivers, highlighting the need for further study on the prevalence and intensity of freshwater feeding. Stomach content and stable isotope analyses revealed that some larger native Atlantic salmon and brown trout juveniles consumed pink salmon juveniles, complicating assessments of their overall impact on native salmonids.