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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2014

Abstract

Chrysanthemum stunt viroid (CSVd) was first reported in US in the 1940s and is widespread in the world wherever chrysanthemum is grown. Cryotherapy of shoot tips, a new biotechnology developed in the recent years, is a novel application of plant cryopreservation techniques that allows pathogen eradication at a high frequency. Existing studies have proven that this technique can efficiently eradicate pathogens such as virus, phytoplasma and bacterium. However, up to now, there has been no report on viroid eradication. In the present study, we attempted to establish a droplet vitrification cryotherapy method for Argyranthemum and to apply it to eradicate CSVd. Results obtained so far demonstrated that cryotherapy of shoot tips alone failed to eradicate CSVd from the infected shoot tips of Argyranthemum maderense ‘Yellow Empire’. Using in situ hybridization of CSVd and histological analysis, we found that CSVd can invade meristematic cells and at the same time, these cells were able to survive following cryotherapy. These findings explained why cryotherapy of shoot tips alone could not be efficient enough to eradicate CSVd from the diseased materials. Further studies combining cold treatment with cryotherapy are under investigation for CSVd eradication.

2013

To document

Abstract

Human–bear conflicts occur frequently in the Pasvik Valley, Norway. We used a variantof the hair-trapping method with higher densities of traps (2.5 x 2.5 km grid) todetect brown bears moving near human settlements and livestock. We distributed 20hair traps for one month close to a farm with frequent observations of grazing bears.The study area consisted of one area close to the farm, and one adjacent area withoutsettlements. We collected 85 hair samples and identified 13 different individuals bySTR analysis. In the farm area, we detected 4 different males once, and a female thatwas detected in both areas. In comparison, nine bears (2 males and 7 females) weredetected for more than one week in the area without settlements, suggesting lowerroaming activity. Conclusively, hair trapping has the potential to survey bears at specificlocations of importance to the wildlife management.