Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2021
Authors
Fatima Heinicke Xiangfu Zhong Siri Tennebø Flåm Johannes Breidenbach Magnus Leithaug Marthe Thoresen Mæhlen Siri Lillegraven Anna-Birgitte Aga Ellen Sauar Norli Maria Dahl Mjaavatten Espen Andre Haavardsholm Manuela Zucknick Simon Rayner Benedicte Alexandra LieAbstract
Rheumatoid arthritis (RA) is a complex disease with a wide range of underlying susceptibility factors. Recently, dysregulation of microRNAs (miRNAs) in RA have been reported in several immune cell types from blood. However, B cells have not been studied in detail yet. Given the autoimmune nature of RA with the presence of autoantibodies, CD19+ B cells are a key cell type in RA pathogenesis and alterations in CD19+ B cell subpopulations have been observed in patient blood. Therefore, we aimed to reveal the global miRNA repertoire and to analyze miRNA expression profile differences in homogenous RA patient phenotypes in blood-derived CD19+ B cells. Small RNA sequencing was performed on CD19+ B cells of newly diagnosed untreated RA patients (n=10), successfully methotrexate (MTX) treated RA patients in remission (MTX treated RA patients, n=18) and healthy controls (n=9). The majority of miRNAs was detected across all phenotypes. However, significant expression differences between MTX treated RA patients and controls were observed for 27 miRNAs, while no significant differences were seen between the newly diagnosed patients and controls. Several of the differentially expressed miRNAs were previously found to be dysregulated in RA including miR-223-3p, miR-486-3p and miR-23a-3p. MiRNA target enrichment analysis, using the differentially expressed miRNAs and miRNA-target interactions from miRTarBase as input, revealed enriched target genes known to play important roles in B cell activation, differentiation and B cell receptor signaling, such as STAT3, PRDM1 and PTEN. Interestingly, many of those genes showed a high degree of correlated expression in CD19+ B cells in contrast to other immune cell types. Our results suggest important regulatory functions of miRNAs in blood-derived CD19+ B cells of MTX treated RA patients and motivate for future studies investigating the interactive mechanisms between miRNA and gene targets, as well as the possible predictive power of miRNAs for RA treatment response.
Abstract
No abstract has been registered
Authors
Teresa Gómez de la Bárcena Lise Dalsgaard Line Tau Strand Christian Wilhelm Mohr Knut Bjørkelo Rune Eriksen Gunnhild SøgaardAbstract
No abstract has been registered
Abstract
No abstract has been registered
2020
Authors
Stefano Puliti Marius Hauglin Johannes Breidenbach Paul M. Montesano C.S.R. Neigh Johannes Rahlf Svein Solberg Torgeir Ferdinand Klingenberg Rasmus AstrupAbstract
No abstract has been registered
Authors
Johannes BreidenbachAbstract
No abstract has been registered
Authors
Johannes Breidenbach Janis Ivanovs Annika Kangas Mats Nilsson Thomas Nord-Larsen Rasmus AstrupAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Johannes Breidenbach Lars T. Waser Misganu Debella-Gilo Johannes Schumacher Johannes Rahlf Marius Hauglin Stefano Puliti Rasmus AstrupAbstract
No abstract has been registered