Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2017
Abstract
Sea-level rise caused by climate change and global warming, poses a major threat to coastal regions due to salt-water intrusion and damage to agricultural land. Researchers now urge policy makers to take action before it is too late.
Authors
Andre van Eerde Yanliang Wang John-Kristian Jameson Lisa Paruch Aniko Varnai Hege Særvold Steen Vincentius Gerardus Henricus Eijsink Jihong Liu ClarkeAbstract
No abstract has been registered
Authors
Jihong Liu Clarke Lisa Paruch Mihaela-Olivia Dobrica Iuliana Caras Catalin Tucureanu Adrian Onu Sonya Ciulean Crina Stavaru Andre van Eerde Hege Særvold Steen Sissel Haugslien Catalina Petrareanu Catalin Lazar Ioan Popescu Ralph Bock Jean Dubuisson Norica Branza-NichitaAbstract
No abstract has been registered
Authors
Jihong Liu Clarke Lisa Paruch Mihaela-Olivia Dobrica Iuliana Caras Catalin Tucureanu Adrian Onu Sonya Ciulean Crina Stavaru Andre van Eerde Yanliang Wang Hege Særvold Steen Sissel Haugslien Catalina Petrareanu Catalin Lazar Costin-loan Popescu Ralph Bock Jean Dubuisson Norica Branza-NichitaAbstract
The hepatitis C virus (HCV) is a major etiologic agent for severe liver diseases ( e.g . cirrhosis, fibrosis and hepatocellular carcinoma). Approximately 140 million people have chronic HCV infections and about 500 000 die yearly from HCV-related liver pathologies. To date, there is no licensed vaccine available to prevent HCV infection and production of a HCV vaccine remains a major challenge. Here, we report the successful production of the HCV E1E2 heterodimer, an important vaccine candidate, in an edible crop (lettuce, Lactuca s ativa ) using Agrobacterium - mediated transient expression technology. The wild-type dimer (E1E2) and a variant without an N-glycosylation site in the E2 polypeptide (E1E2 Δ N6) were expressed, and appropriate N-glycosylation pattern and functionality of the E1E2 dimers were demonstrated. The humoral immune response induced by the HCV proteins was investigated in mice following oral administration of lettuce antigens with or without previous intramuscular prime with the mammalian HEK293T cell-expressed HCV dimer. Immunization by oral feeding only resulted in development of weak serum levels of anti-HCV IgM for both antigens; however, the E1E2 Δ N6 proteins produced higher amounts of secretory IgA, suggesting improved immunogenic properties of the N-glycosylation mutant. The mice group receiving the intramuscular injection followed by two oral boosts with the lettuce E1E2 dimer developed a systemic but also a mucosal immune response, as demonstrated by the presence of anti-HCV secretory IgA in faeces extracts. In summary, our study demonstrates the feasibility of producing complex viral antigens in lettuce, using plant transient expression technology, with great potential for future low-cost oral vaccine development.
Abstract
The moisture status of the upper 10cm of the soil profile is a key variable for the prediction of a catchment's hydrological response to precipitation, and of pivotal importance to the estimation of trafficability. Prediction, and even mapping, of topsoil water content is complicated, not in the least because of its large spatial heterogeneity. In IRIDA, an EU/JPI project, measurements, models and weather predictions will be applied to estimate the soil moisture status at the sub-field scale in near-real time. The project is in its early stages, during which the relevant parameters will be selected that will allow for soil moisture mapping on agricultural fields at a 10 m resolution.
Authors
Jihong Liu Clarke Andre van Eerde Lisa Paruch Inger Heldal Hege Særvold Steen Yanliang Wang Astrid Sivertsen Sissel HaugslienAbstract
No abstract has been registered
Abstract
Area-efficient constructed systems for stormwater management and bioretention may involve large fluc-tuations in subsurface water levels. Such fluctuations challenge vegetation by forcing roots to exploredeeper layers to access water during dry periods. In a controlled experiment, we studied growth pat-terns and the ability of Phragmites australis roots to track subsurface water level fluctuations of differingamplitude and frequency in substrates with contrasting water-holding capacity. We found that P. aus-tralis was able to adjust its rooting pattern to considerable subsurface water level fluctuations (to wellbelow 120 cm), but that substrate characteristics can restrict its ability to adjust to larger fluctuations.Fluctuation amplitude was the driving factor for plant growth and biomass allocation responses, whilesubstrate characteristics and fluctuation frequency were less important. When not exposed to large waterlevel fluctuations, P. australis grew larger shoots and only explored intermediate rooting depths. Therewas a negative relationship between root and rhizome biomass, showing a resource-based trade-off andshort-term costs of adjusting rooting patterns to large water level fluctuations. These results indicatethat P. australis is suited for systems with considerable subsurface water fluctuations, but constraints onits flexibility need to be investigated.
Authors
Synnøve Rivedal Samson Øpstad Sverre Heggset Sissel Hansen Trond Børresen Torbjørn Haukås Johannes Deelstra Peter DörschAbstract
No abstract has been registered
Authors
Synnøve Rivedal Samson Øpstad Sverre Heggset Trond Børresen Torbjørn Haukås Sissel Hansen Peter Dörsch Johannes DeelstraAbstract
No abstract has been registered
2016
Abstract
No abstract has been registered