Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2017
Authors
Yusuke Satoh Taher Kahil Edward Byers Peter Burek Günther Fischer Sylvia Tramberend Peter Greve Martina Flörke Stephanie Eisner Naota Hanasaki Piotr Magnuszewski Luzma Fabiola Nava William Cosgrove Simon Langan Yoshihide WadaAbstract
No abstract has been registered
Authors
Ines Figueira Lucelia Tavares Carolina Jardim Ines Costa Ana P. Terrasso Andreia F. Almeida Coen Govers Jurriaan J. Mes Rui Gardner Jörg D. Becker Gordon J. McDougall Derek Stewart Augusto Filipe Kwang S. Kim Dora Brites Catarina Brito Maria Alexandra Brito Claudia N. SantosAbstract
Purpose Epidemiological and intervention studies have attempted to link the health effects of a diet rich in fruits and vegetables with the consumption of polyphenols and their impact in neurodegenerative diseases. Studies have shown that polyphenols can cross the intestinal barrier and reach concentrations in the bloodstream able to exert effects in vivo. However, the effective uptake of polyphenols into the brain is still regarded with some reservations. Here we describe a combination of approaches to examine the putative transport of blackberry-digested polyphenols (BDP) across the blood–brain barrier (BBB) and ultimate evaluation of their neuroprotective effects. Methods BDP was obtained by in vitro digestion of blackberry extract and BDP major aglycones (hBDP) were obtained by enzymatic hydrolysis. Chemical characterization and BBB transport of extracts were evaluated by LC–MSn. BBB transport and cytoprotection of both extracts was assessed in HBMEC monolayers. Neuroprotective potential of BDP was assessed in NT2-derived 3D co-cultures of neurons and astrocytes and in primary mouse cerebellar granule cells. BDP-modulated genes were evaluated by microarray analysis. Results Components from BDP and hBDP were shown to be transported across the BBB. Physiologically relevant concentrations of both extracts were cytoprotective at endothelial level and BDP was neuroprotective in primary neurons and in an advanced 3D cell model. The major canonical pathways involved in the neuroprotective effect of BDP were unveiled, including mTOR signaling and the unfolded protein response pathway. Genes such as ASNS and ATF5 emerged as novel BDP-modulated targets. Conclusions BBB transport of BDP and hBDP components reinforces the health benefits of a diet rich in polyphenols in neurodegenerative disorders. Our results suggest some novel pathways and genes that may be involved in the neuroprotective mechanism of the BDP polyphenol components.
Authors
Trond Løvdal Ferruh Erdogu Bart van Droogenbroeck Ingunn M. Vågen Agnieszka Bartoszek Christine Vos Inge Hanssen Giovanni Agati Stanislaw Kaniszewski Dagbjørn Skipnes Mustafa Tutar Romain Larbat Christophe RobinAbstract
No abstract has been registered
Authors
Amilia Vranac Asima Akagić Fuad Gasi Nermina Spaho Mekjell MelandAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Anna Kårlund Kati Hanhineva Marko Lehtonen Gordon J. McDougall Derek Stewart Reijo O. KarjalainenAbstract
BACKGROUND: The non-edible parts of horticultural crops, such as leaves, contain substantial amounts of valuable bioactive compounds which are currently only little exploited. For example, strawberry (Fragaria × ananassa) leaves may be a promising bioresource for diverse health-related applications. However, product standardization sets a real challenge, especially when the leaf material comes from varying cultivars. The first step towards better quality control of berry fruit leaf-based ingredients and supplements is to understand metabolites present and their stability in d ifferent plant cultivars, so this study surveyed the distribution of potentially bioactive strawberry leaf metabolites in six different strawberry cultivars. Non-targeted metabolite profiling analysis using LC/qTOF-ESI-MS with data processing via principal component analysis and k-means clustering analysis was utilized to examine differences and commonalities between the leaf metabolite profiles. RESULTS: Quercetin and kaempferol derivatives were the dominant flavonol groups in strawberry leaves. Previously described and novel caffeic and chlorogenic acid derivatives were among the major phenolic acids. In addition, ellagitannins were one of the distinguishing compound classes in strawberry leaves. In general, strawberry leaves also contained high levels of octadecatrienoic acid derivatives, precursors of valuable odour compounds. CONCLUSION: The specific bioactive compounds found in the leaves of different strawberry cultivars offer the potential for the selection of optimized leaf materials for added-value food and non-food applications.
Authors
Anna Bucharova Walter Durka Norbert Hölzel Johannes Kollmann Stefan Michalski Oliver BossdorfAbstract
No abstract has been registered
Authors
Janne O. Kjønaas Gro HylenAbstract
No abstract has been registered
Authors
Diego Faustolo Alves Bispo Marx Leandro Naves Silva Joao Jose Granate de Sa e Melo Marques Marianne Bechmann Pedro Velloso Gomes Batista Nilton CuriAbstract
No abstract has been registered
Authors
I. Figueira G. Garcia R.C. Pimpao A.P. Terrasso I. Costa A.F. Almeida L. Tavares T.F. Pais P. Pinto M.R. Ventura A. Filipe G. J. McDougall Derek Stewart K.S. Kim I. Palmela D. Brites M.A. Brito C. Brito C. N. SantosAbstract
No abstract has been registered