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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2025

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Abstract

Seed moisture content (SMC) is the most reliable indicator of optimal harvest timing in many seed crops, including grass seed. Measuring SMC as grass seed crops approach maturity is recommended to determine optimal harvest timing. Currently, to measure SMC, seeds need to be stripped from heads by hand, weighed, dried until all the moisture has been lost, then re-weighed, and SMC manually calculated. Collecting SMC information in this manner is arduous, time consuming, and prone to error. Consequently, this procedure has resulted in inadequate SMC testing or failure to test in a timely fashion. In addition, SMC is an important factor in the storage of harvested seed, which typically needs to be stored under 12% SMC, to ensure high seed quality. The goal of this project is to develop a portable sensing device - The Grady Sensor - that allows for rapid and accurate SMC measurement of grass seed. The sensor employs near-infrared (NIR) spectroscopy principles that water molecules absorb specific NIR wavelengths. By analysing the light reflected from the seed surface, the sensor predicts SMC based on the intensity of the reflected light at moisture-sensitive wavelengths. Over two years, multiple sensor prototypes have been developed, and their performance has been validated through field tests in Oregon and New Zealand. Sensor readings were compared to laboratory oven gravimetric SMC values of samples collected from major grass seed species, including tall fescue, annual ryegrass, perennial ryegrass, orchardgrass, creeping red fescue, creeping bentgrass, Chewings fescue, and Kentucky bluegrass. The sensor readings demonstrated a significant linear relationship with the oven SMC. Mean absolute errors of sensor SMC predictions were within 1.2 to 4.6% across all grass species. The results indicate that the prototype is a reliable replacement for the traditional oven drying method.

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Abstract

Tychius picirostris Fabricius (Coleoptera: Curculionidae), the clover seed weevil (CSW), is a major pest of white clover (Trifolium repens L.) seed crops in Oregon, USA, where larval feeding during seed development reduces yield and quality, causing substantial economic losses. For decades, CSW management has relied on bifenthrin (IRAC Group 3A), but since 2017 growers have reported reduced efficacy, prompting concern of resistance. Laboratory bioassays in 2022-2023 confirmed very high resistance to bifenthrin (RR50 = 178-726) and moderate resistance to malathion (Group 1B; RR50 = 7.8-32.8), underscoring the need for alternative chemistries and insecticide resistance management (IRM) guidelines. From 2022 to 2024, on-farm insecticide efficacy trials were conducted in commercial white clover seed fields in western Oregon. Early-season (pre-bloom or PB) application using contact insecticides (malathion, isocycloseram [Group 30], indoxacarb [Group 22]) targeted adults, either alone or in sequence with mid-season (full bloom or FB) application of systemic insecticides (chlorantraniliprole and cyantraniliprole [Group 28]) targeting larvae. Adult abundance was monitored with 20-sweep net samples, and larval densities were estimated from 30 inflorescences per plot extracted with Berlese funnels. Across sites and years, isocycloseram consistently suppressed adult populations, cyantraniliprole reduced larval densities, while indoxacarb showed variable performance. Although seed yields did not differ significantly among treatments, yet efficacy data supported product registration in Oregon and highlighted the value of chemical rotation plans for resistance management. Based on these findings, we recommend discontinuing bifenthrin and adopting an integrated resistance management (IRM) program that applies contact insecticides during spring adult migration when ≥2 weevils per sweep are detected and systemic insecticides during full bloom when ≥3 per 30 inflorescences are observed.

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Abstract

Orchardgrass (Dactylis glomerata L.) is an important forage seed crop, but unlike other cool-season grass seed crops such as perennial ryegrass and tall fescue , seed yields have not increased over time. Research from the literature suggests that plant growth regulators (PGRs), such as trinexapac-ethyl (TE), and spring nitrogen (N) application increase seed yield in orchardgrass by increasing seed number. However, no research has investigated the effects of PGRs and spring N on orchardgrass seed development. Field trials were conducted in 2018 and 2019 to investigate orchardgrass seed development and the effects of PGR and spring N treatments on this process. Treatments included an untreated control, TE (210 g ai ha-1), spring N (112 kg ha-1), and TE + N. Regression analyses were used to elucidate seed development in three spikelet positions: distal, central, and proximal. In 2018, seed weight increased over growing degree days (GDD) in a bi-phasic segmented pattern from distal and central spikelets, but increases were linear from proximal spikelets. In 2019, seed weight increased in proximal spikelets following a bi-phasic segmented function, and in central spikelets, the seed weight increase was also bi-phasic, except for the TE treatment. Seed growth rate varied among spikelet positions, ranging from 0.22 to 0.34 mg GDD-1 per 100 seeds. The seed growth rate varied among TE and N treatments, ranging from 0.31 to 0.47 mg GDD-1 per 100 seed. The TE + N treatment had the shortest seed filling duration and one of the smallest seed growth rate values, producing low seed weight. The TE + N treatment produced high seed number and seed yield, indicating a reduction in seed abortion or shattering. Seed carbon (C) and N content increased during seed development and peak deposition preceded physiological maturity. There was no effect of TE on deposition of C or N in orchardgrass seed.

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Abstract

Epidemiology and management of aphid-transmitted yellow dwarf viruses (YDVs) have received international attention in small grains, but research regarding YDVs in grass seed production is limited. An integrated pest management program is needed to reduce the impact of YDVs in grass seed crops that are grown for more than one year. The objectives of this work were to: 1) survey commercial grass seed production fields to determine spatiotemporal virus composition, 2) evaluate the effects of nitrogen (N) fertiliser rate, and the timing and frequency of foliar insecticide applications on aphid abundance, YDV disease incidence, and seed yield in two perennial ryegrass cultivars, and 3) develop high-throughput phenotyping methods to screen cultivars for host plant resistance. To determine the incidence and diversity of YDVs, perennial ryegrass (n=20) and tall fescue (n=30) seed fields in Oregon were surveyed in 2021-2022. In 82% of fields, a Luteovirus-type YDV was detected, and 65% had detection of a Polerovirus-type YDV. In small-plot field trials conducted from 2021 to 2024, high N rates increased YDV incidence in perennial ryegrass. Seed yield was greatest for the less susceptible cultivar when protected with one insecticide treatment per season. A higher-than-recommended N rate did not increase seed yield across treatment combinations in first-year stands but did increase seed yields in second and third-year stands when YDV infection was >50%. Phenotyping methods were evaluated to assess potential host-plant resistance to YDVs using perennial ryegrass cultivars (n=27) with high- throughput automated video tracking for aphid behaviours that may confer resistance, and compared to traditional phenotyping methods. Several cultivars showed potential tolerance to YDVs. This research provides new knowledge of the spatial composition of aphid-transmitted YDVs, integrated pest management guidelines, and high-throughput methods for breeding programs to develop cultivars that are resistant to YDVs.

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Abstract

Abstract Winter damage of golf turf in northern environments is a persistent challenge, and reseeding is often necessary to promote recovery and to maintain adequate density and uniformity for play. However, adverse conditions associated with spring seedings can negatively impact reestablishment of creeping bentgrass ( Agrostis stolonifera L.) (CBG) on golf greens, tees, and fairways. The objectives of the research were to examine different strategies to promote rapid reestablishment of CBG in early spring, including cultivar selection, use of a synthetic cover, and application of plant health products. To assess the impacts of CBG cultivars and cover on reestablishment, 12 cultivars and two cover treatments (without or with a permeable synthetic cover) were established at two locations in 2021 (South Deerfield, A, and Saint Paul, MN). The effects of plant health products on CBG establishment were assessed in separate field trials at two locations in 2021 and 2022 (South Deerfield, MA, and Grimstad, Norway). Plant health product treatments were applied on a weekly basis following emergence and included: control (water), chitosan, silica, acibenzolar S‐methyl, glycine betaine, seaweed extracts (alone or together with humic substances), trinexapac‐ethyl, and gibberellic acid. Soil and air temperatures were monitored, and plots were visually assessed for changes in percent green turfgrass cover. The use of a permeable cover increased soil temperatures and decreased the time to achieve 50% turfgrass cover by 7–12 days, depending on location and regardless of cultivar. Compared to effect of covering treatment, most CBG did not significantly vary in spring establishment rates, except for Independence, which exhibited slower establishment. Among the various plant health products tested over 2 years and two locations, we did not identify any specific product that consistently enhanced early spring establishment of CBG.

Abstract

Abstract Red fescue (RF, Festuca rubra L.) is one of the most widely used turfgrass species in Europe. The subspecies Chewings fescue (CF) and slender creeping red fescue (SlCRF) are used on putting greens because they tolerate a mowing height of 4–5 mm. Previous research has shown CF to be more winter‐hardy than SlCRF, however, SlCRF has a greener winter color. Our objective was to find the optimal weight ratio of the two subspecies in seed blends for greens in contrasting climatic regions of the Nordic countries. Musica and Cezanne (reference varieties in SCANGREEN variety testing since 2012) were selected as representatives for CF and SlCRF, respectively. Seed blends including CF/SlCRF in weight ratios of 75/25, 50/50, and 25/75 were compared to pure CF and SlCRF at Sydsjælland GC, Denmark, NIBIO Landvik, Norway, NIBIO Apelsvoll, Norway, and Reykjavik GC, Iceland as an add‐on to SCANGREEN variety testing 2015–2018. Results showed the optimal blends to produce higher turfgrass quality and tiller density than monocultures of either subspecies at all sites except Apelsvoll where plots were killed during the first winter. The best‐performing CF/SlCRF blend was 50/50 at Sydsjælland and Landvik and 75/25 at Reykjavik. While this shows seed blends to have advantages compared with the pure subspecies, the study should be repeated with a focus on dollar spot ( Clarireedia spp.), which has become a major turfgrass disease in Southern Scandinavia since these trials were conducted.