Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2010
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Authors
Hsin-Ho Huang Daniel Camsund Peter Lindblad Thorsten HeidornAbstract
Cyanobacteria are suitable for sustainable, solar-powered biotechnological applications. Synthetic biology connects biology with computational design and an engineering perspective, but requires efficient tools and information about the function of biological parts and systems. To enable the development of cyanobacterial Synthetic Biology, several molecular tools were developed and characterized: (i) a broad-host-range BioBrick shuttle vector, pPMQAK1, was constructed and confirmed to replicate in Escherichia coli and three different cyanobacterial strains. (ii) The fluorescent proteins Cerulean, GFPmut3B and EYFP have been demonstrated to work as reporter proteins in cyanobacteria, in spite of the strong background of photosynthetic pigments. (iii) Several promoters, like P(rnpB) and variants of P(rbcL), and a version of the promoter P(trc) with two operators for enhanced repression, were developed and characterized in Synechocystis sp. strain PCC6803. (iv) It was shown that a system for targeted protein degradation, which is needed to enable dynamic expression studies, is working in Synechocystis sp. strain PCC6803. The pPMQAK1 shuttle vector allows the use of the growing numbers of BioBrick parts in many prokaryotes, and the other tools herein implemented facilitate the development of new parts and systems in cyanobacteria.
Authors
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Brita Toppe Anne Marte Tronsmo Bjørn Økland Leif SundheimAbstract
No abstract has been registered
Authors
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Brita Toppe Anne Marte Tronsmo Bjørn Økland Leif SundheimAbstract
No abstract has been registered
Authors
Arild Andersen Trond Hofsvang May Bente Brurberg Christer Magnusson Trond Rafoss Anne Marte Tronsmo Bjørn Økland Leif SundheimAbstract
No abstract has been registered
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Authors
Ana Maria Barata da Silva Åsmund Asdal Elinor LipmanAbstract
No abstract has been registered