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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2010

Abstract

There is an increasing need for forest resource monitoring methods, as more attention is paid to deforestation, bio-energy and forests as habitats. Most national forest inventories are based on networks of field inventory plots, sometimes together with satellite data, and airborne laser scanning (ALS) is increasingly used for local forest mapping. These methods are expensive to establish or carry out, and many countries, including some severely affected by deforestation, do not apply such methods.Satellite based remote sensing methods in use today are hampered by problems caused by clouds and saturation at moderate biomass levels. Satellite SAR is not hampered by cloud problems, and monitoring of canopy surface elevation, which is correlated to key forest resource variables, might be a future method in forest monitoring.We here present the main findings of three studies (Solberg et al. 2010, a, b, c) investigating the potential of interferometric SAR (InSAR) for forest monitoring, by describing the relationship between InSAR height above ground and key forest variables. We based this study on InSAR data from the Shuttle Radar Topographic Mission (SRTM) with its acquisition in February 2000. We obtained SRTM InSAR DEM data from DLR for two forest areas in Norway, and built a ground-truth from the combination of field inventory and ALS.The forest areas were dominated by Norway spruce and Scots pine. In each forest area we laid out a number of field inventory plots, where we recorded standard forest variables such as Dbh and tree height, and from this derived plot aggregated variables of top height, mean height, stand density (mean tree height divided by the mean tree spacing), volume and biomass. We used this to calibrate and validate ALS based models, from which we derived estimates of the same variables for each SRTM pixel. This served as reference data for the SRTM data.From the X-band SRTM digital surface model (DSM) image we subtracted a high quality digital terrain model (DTM) derived from the ALS data. This was based on an extraction of ground echoes from the data provider, and the elevations of these echoes were interpolated into a grid fitting the SRTM grid.This produced data on the RADAR echo height above ground (InSAR height), which we related to the forest variables. With digital stand maps we aggregated the variables to the stand level. The X-band microwaves penetrate a little into the canopy, and the InSAR height was on average about 1.2 m below the mean tree height. InSAR height was strongly related to all forest variables, most strongly to top height.Particularly valuable was that stem volume and biomass, ranging up to 400 m3/ha and 200 t/ha, respectively, were linearly related to InSAR height with an accuracy, RMSE, of 19% at the stand level. However, these relationships had an intercept, which represents the microwave penetration into the vegetation, and due to this the relationships were non-linear for forest stands having heights and biomass values close to zero.With a lower quality DTM derived from topographic maps, the relationships were weaker. However, as long as a forest variable is within the ranges of the linear relationship, any change in InSAR elevation would be proportional to a change in forest height, volume or biomass. And, any logging should be detectable as a sudden decrease in InSAR elevation.Hence, a forest monitoring based on X-band InSAR might be suitable even without a DTM. An application of space borne InSAR for forest monitoring would be feasible for large areas at low cost, whereas an ALS acquisition for a part of the area would serve as reference data for calibration.

Abstract

The genome H. annosum s.l. was sequenced by JGI to a 8.23X coverage and assembled into 39 scaffolds with a total size of 33.7 Mb covering more than 98% of the whole genome. Based of genome sequence we annotated a number of genes for fungal enzymes that are believed to participate in lignin degradation, including: laccases (Lcc18 genes), manganese peroxidases (MnP8 genes) and hydrogen peroxide forming enzymes such as glyoxal oxidases (GLOX5 genes), quinone oxidoreductases (QOR17 genes) and aryl alcohol oxidases (AAO16 genes), which is in concordance with these gene family sizes observed in other sequenced white-rot fungi. We studied the genomic organisation and phylogeny of these genes as well as their expression using NimbleGen arrays and qRT-PCR. Transcript profiling using whole-genome oligo arrays and qRT-PCR revealed that some transcripts were very abundant in lignin-rich media (Lcc5 15, MnP2, GLOX4, QOR2 10, AAO9), in cellulose-rich media (lcc2, 7 16, MnP3 4, GLOX3, QOR4 6, AAO2, 7 10), in wood (Lcc3, MnP4, QOR2, GLOX1, AAO10) or in the free-living mycelium grown liquid culture (Lcc1, 3, 10 13), suggesting specific functions of these genes, which need to be studied further.

Abstract

In 1955 the potato cyst nematode (PCN) was recorded for the first time in Norway. This detection resulted in extensive surveys and measures were implemented based on the statutory regulation of 1916. The first statutory regulation for PCN was put in power in 1956, and later amended in several occasions. These regulations prohibit the introduction and spread of PCN with soil and plant materials. Early control strategies included the use of chemical fumigants and resistant potato cultivars in infested fields, and surveys detected new infestations which were placed under quarantine regulations. The recognition of G. rostochiensis and G. pallida, their pathotypes enabled a more precise use of resistant cultivars. Commercial chemical fumigants, organophosphates or carbamate nematicides have not been used in Norway since the early 1970s. Today, non-virulent G. rostochiensis is managed by crop rotation, while infestations by G. pallida or virulent G. rostochiensis results in at least 40-years ban for growing potato. Most Norwegian potato cultivars have the resistance genes, Gro-1 (H1) from Solanum tuberosum ssp. andigena. During the preceding decades great emphasis has been placed on documenting freedom from PCN in the production of certified seed potatoes, certified seed potato are used in combination with crop rotations using non-host crops, alternating susceptible and resistant cultivars. These are important control measures, but not easy to implement in Norway due to restricted acreage suitable for long rotations. The safe use of resistant potato cultivars requires a better knowledge on the presence of species and pathotypes in potato fields. In order to improve our information of the occurrence of PCN a new national survey program for the principal potato districts has started. These surveys will complemented by information generated from a new research project dealing with: studies of the virulence of selected PCN populations, decline rates of nematode field population densities and infection potential over time of populations from fields placed under quarantine regulations. studies on the occurrence and pathogenicity of microbial antagonistic parasitic on PCN, and their potential of future management of PCN, the safe use of early potato cultivars as a practical control method, and the potential for using Solanum sisymbriifolium as a trap crop, distinguish the degree of resistance of selected potato varieties available on the Norwegian market, and initial studies of the PCN-Potato-Pathosystem. These expected results of this project possibly will improve the management of PCN, and may alleviate present regulatory restrictions.

Abstract

Scenarios of climate changes indicate longer and more frequent spells of mild weather during winter in northern latitudes. De-hardening in perennial grasses could increase the risk of frost kill. In this study, the resistance to de-hardening of different grass species and cultivars was examined, and whether the resistance changes during winter or between years, was tested. In Experiment 1, two cultivars of timothy (Phleum pratense L.) and perennial ryegrass (Lolium perenne L.) of contrasting winter hardiness were grown under ambient winter conditions, transferred from the field in January and April 2006 to the laboratory for 9 d with controlled de-hardening conditions of 3°C, 9°C and 15°C. The timothy cultivars were tested at 3°C, 6°C and 9°C in a similar experiment (Experiment 2) in January 2007. De-hardening, measured as decrease in frost tolerance (LT50), was less in timothy than in perennial ryegrass and increased with increasing temperatures. The northern winter-hardy cultivar Engmo of timothy de-hardened more rapidly than the less-hardy cultivar Grindstad, but had higher initial frost tolerance in both experiments, whereas there was less difference between cultivars of perennial ryegrass in Experiment 1. Cultivar Grindstad of timothy lost all hardiness in early spring at all temperatures, whereas cultivar Engmo maintained some hardiness at 3°C. Cultivar Engmo de-hardened at a lower rate in 2007 than in 2006, in spite of similar frost tolerance at the start of de-hardening treatment in both years. This indicates that the rate of de-hardening was controlled by factors additional to the initial frost tolerance and that autumn weather conditions might be important for the resistance to de-hardening.