Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2012
Authors
Igor A. Yakovlev YeonKyeong Lee Bjørn Rotter Tore Skrøppa Øystein Johnsen Jorunn Elisabeth Olsen Carl Gunnar FossdalAbstract
Epigenetic memory marks establishment in Norway spruce occur exclusively during embryogenesis in response to environmental impact, and the epitype is fixated by the time the embryo is fully developed without a change in the DNA sequence. We started large scale studies aimed on identifying and characterizing of genes and regulatory elements involved in the initiation, maintenance, and heritability of epigenetic memory using candidate genes and next generation sequencing approaches. Molecular mechanisms of formation of epigenetic memory were studied on the same full-sibs family zygotic embryo in vitro cultures developed in cold (18°C) and warm (30°C) environmental conditions from proliferation till mature embryo stages. Initially we had found large set (64) of Arabidopsis epigenetic regulator gene homologs in spruce. In general, known epigenetic related genes are very well represented among spruce ESTs. Analysis of the transcription patterns of these genes using RT-PCR in epigenetically different embryogenic samples reveal specific transcription patterns on different stages of embryogenic development dependent on epitype. We are expecting to determine certain stages during embryogenesis when epigenetic memory marks are forming. At the same time, nearly no differences in transcription levels of studied genes had been found in seedlings (4 month old), originated from full-sib families clearly differed in epigenetic response. Using MACE (massive cDNA 3-end sequencing) deep mRNA sequencing on the Illumina GSII platform, we analyzed P. abies transcriptomes by comparison warm and cold originated “embryonic epitypes” developed in cold and warm environmental conditions. Significant differences in transcriptomes between epitypes revealed by high-throughput sequencing will be discussed.
Abstract
The liverwort Trichocolea tomentella is known from 65 localities in southern Norway. Almost half of these are in Hordaland county in Western Norway. T. tomentella has been observed at many localities (42%) in the last decade. On the other hand it has not been observed at 23% of the previously known localities after 1950, although a complete re-survey has not been performed. The species seems to be thriving in its main distribution centre in Western Norway, but in southeastern Norway urbanisation and modern forestry are major threats. In this part of Norway the species has probably gone extinct or is on its way to extinction at several of the localities. To preserve the species logging and draining close to springs, streams and gullies should be ceased. This will also preserve habitats that are species rich, in particular in bryophytes.
Authors
David Bell David G. Long Alan D. Forrest Michelle L. Hollingsworth Hans Blom Peter M. HollingsworthAbstract
DNA barcoding of a group of European liverwort species from the genus Herbertus was undertaken using three plastid (matK, rbcL and trnH-psbA) and one nuclear (ITS) marker. The DNA barcode data were effective in discriminating among the sampled species of Herbertus and contributed towards the detection of a previously overlooked European Herbertus species, described here as H. norenus sp. nov. This species shows clear-cut differences in DNA sequence for multiple barcode regions and is also morphologically distinct. The DNA barcode data were also useful in clarifying taxonomic relationships of the European species with some species from Asia and North America. In terms of the discriminatory power of the different barcode markers, ITS was the most informative region, followed closely by matK. All species were distinguishable by ITS alone, rbcL + matK and various other multimarker combinations.
Abstract
Springs are characterized by consistent thermal and hydrologic conditions, which enable use of spring-inhabiting organisms as sensitive indicators of biogeochemical changes in their catchments. We hypothesized that bryophytes would show a stronger response than vascular plants to changes in spring water quality because submerged bryophytes do not take up compounds from the soil. We analyzed species responses to interannual changes in spring water quality (discharge, water temperature, electrical conductivity, and pH) in 57 forest springs over 4 consecutive years. We calculated interannual turnover in species composition for bryophytes and vascular plants with the Bray–Curtis dissimilarity index. We applied regression analysis to test interannual changes in species composition of the taxonomic groups over time, and we used 2-sided t-tests to compare year-to-year changes in species composition between bryophytes and vascular plants. We used boosted regression tree (BRT) models to quantify the relative importance of different physicochemical variables and Pearson linear correlation to quantify short-term changes in vegetation relative to changes in spring-water pH. For both groups, interannual changes in species composition were significantly positively related to time scale. Bryophytes did not show a significantly stronger response than vascular plants to interannual changes in the environment. Alterations in pH and conductivity explained most of the observed interannual changes in species composition of both groups, whereas changes in water temperature and discharge were less important. However, responses of single species to environmental change may be delayed, resulting in inertia at the community and ecosystem scales. Hence, longer time periods need to be considered for a better understanding of response times of the vegetation of European forest springs to changes in spring water quality.
Abstract
No abstract has been registered
Authors
Toril Drabløs Eldhuset Nina Elisabeth Nagy Daniel Volařík Isabella Børja Roman Gebauer Igor A. Yakovlev Paal KrokeneAbstract
Purpose: Drought-induced tree susceptibility is a major risk associated with climate change. Here we report how an 11-week drought affected tracheid structure, gene expression, and above- and belowground growth in 5-year-old Norway spruce trees (Picea abies) under controlled conditions. Results: The canopy of trees subjected to severe drought had significantly less current-year needle biomass, and fewer tracheids and tracheid rows in current-year shoots compared to fully watered control trees. Belowground tissues were more strongly affected by drought than aboveground tissues. In fine roots (<2 mm diameter) severe drought significantly reduced root biomass, root diameter, root length density and root surface area per soil volume compared to the control. Tracheid diameter and hydraulic conductivity in fine roots were significantly lower and tracheid flatness higher in trees subjected to severe drought than in control trees, both for long and short roots. Transcripts of the drought-related dehydrins PaDhn1 and PaDhn6 were strongly upregulated in stem bark and current-year needles in response to drought, whereas PaDhn4.5 was down-regulated. Conclusions: This study demonstrates that drought reduces biomass and hydraulic conductivity in fine roots and needles. We suggest that the ratio between PaDhn6 and PaDhn4.5 may be a sensitive marker of drought stress in Norway spruce.
Authors
Toril Drabløs Eldhuset Isabella Børja Roman Gebauer Paal Krokene Nina Elisabeth Nagy Josef Urban Daniel VolarikAbstract
No abstract has been registered
Authors
Christian Brischke Linda Meyer Gry Alfredsen Miha Humar Lesley Francis Per Otto Flæte Pia Larsson-BrelidAbstract
The material-inherent resistance of wood is one of the most important qualities influencing the durability of timber. Hence, it has also a major effect on the service life to be expected from a timber construction. In addition, design details and the respective climatic conditions determine durability and make it impossible to treat wood durability as an absolute value. Moreover, the reference magnitude varies between locations because of climatic differences. Durability classification is therefore based on comparing a certain performance indicator between the timber in question and a reference timber. Finally, the relative values (= resistance factors) are grouped and related to durability classes, which can refer to a high range of service lives for a certain location. The insufficient comparability of such durability records turned out to be a key problem for the service life prediction of timber structures, even when the climatic conditions are clearly defined. This study aimed therefore on an inventory of literature data, directly based on service life measures, not masked by a durability classification schedule. It focused on natural durability of timber tested in the field under above-ground conditions. In total 395 durability recordings from 31 different test sites worldwide and based on ten different test methods have been considered for the calculation of resistance factors: 190 for hardwoods and 205 for softwoods. Nevertheless, the considered datasets were heterogeneous in quality and quantity; the resulting resistance factors suffered from high variation. In many cases information was presented too condensed and incompletely, which is inescapable for instance in journal articles. To increase the amount of available, comparable, and directly service-life related data a reliable platform is needed. A proposal for a corresponding data base is provided in part 2 of this paper.
Abstract
A proposal for a web-based platform for scientific exchange of test data in the field of wood durability and wood protection has been made. The overall aim of the durability data base is to improve the usability of existing test data and to create an added value for durability research and service life prediction. The database allows for test results from standardized and non-standardized laboratory and field tests. Natural durable timber, preservative and water-repellent treated timber, chemically and thermally modified timber as well as composites have been considered. Finally all types of decay organisms and other degrading agents are regarded.
Authors
Franҫois Lefèvre Jarkko Koskela Jason Hubert Hojka Kraigher Roman Longauer Ditte C. Olrik Silvio Schüler Michele Bozzano Paraskevi Alizoti Remigijus Bakys Cathleen Baldwin Dalibor Ballian Sanna Black-Samuelsson Dagmar Bednarova Sándor Bordács Eric Collin Bart De Cuyper Sven M.G. De Vries Thröstur Eysteinsson Josef Frýdl Michaela Haverkamp Mladen Ivankovic Heino Konrad Czeslaw Koziol Tiit Maaten Eduardo Notivol Paino Hikmet Öztürk Ivanova Denitsa Pandeva Gheorghe Parnuta Andrej Pilipovic Dragos Postolache Cathal Ryan Arne Steffenrem Maria Carolina Varela Federico Vessella Roman T. Volosyanchuk Marjana Westergren Frank Wolter Leena Yrjänä Inga ZarinaAbstract
Dynamic conservation of forest genetic resources (FGR) means maintaining the genetic diversity of trees within an evolutionary process and allowing generation turnover in the forest. We assessed the network of forests areas managed for the dynamic conservation of FGR (conservation units) across Europe (33 countries). On the basis of information available in the European Information System on FGR (EUFGIS Portal), species distribution maps, and environmental stratification of the continent, we developed ecogeographic indicators, a marginality index, and demographic indicators to assess and monitor forest conservation efforts. The pan-European network has 1967 conservation units, 2737 populations of target trees, and 86 species of target trees. We detected a poor coincidence between FGR conservation and other biodiversity conservation objectives within this network. We identified 2 complementary strategies: a species-oriented strategy in which national conservation networks are specifically designed for key target species and a site-oriented strategy in which multiple-target units include so-called secondary species conserved within a few sites. The network is highly unbalanced in terms of species representation, and 7 key target species are conserved in 60% of the conservation units. We performed specific gap analyses for 11 tree species, including assessment of ecogeographic, demographic, and genetic criteria. For each species, we identified gaps, particularly in the marginal parts of their distribution range, and found multiple redundant conservation units in other areas. The Mediterranean forests and to a lesser extent the boreal forests are underrepresented. Monitoring the conservation efficiency of each unit remains challenging; however, <2% of the conserved populations seem to be at risk of extinction. On the basis of our results, we recommend combining species-oriented and site-oriented strategies.