Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2017
Abstract
In fungi, distribution of secondary metabolite (SM) gene clusters is often associated with host- or environment-specific benefits provided by SMs. In the plant pathogen Alternaria brassicicola (Dothideomycetes), the DEP cluster confers an ability to synthesize the SM depudecin, a histone deacetylase inhibitor that contributes weakly to virulence. The DEP cluster includes genes encoding enzymes, a transporter, and a transcription regulator. We investigated the distribution and evolution of the DEP cluster in 585 fungal genomes and found a wide but sporadic distribution among Dothideomycetes, Sordariomycetes, and Eurotiomycetes. We confirmed DEP gene expression and depudecin production in one fungus, Fusarium langsethiae. Phylogenetic analyses suggested 6–10 horizontal gene transfers (HGTs) of the cluster, including a transfer that led to the presence of closely related cluster homologs in Alternaria and Fusarium. The analyses also indicated that HGTs were frequently followed by loss/pseudogenization of one or more DEP genes. Independent cluster inactivation was inferred in at least four fungal classes. Analyses of transitions among functional, pseudogenized, and absent states of DEP genes among Fusarium species suggest enzyme-encoding genes are lost at higher rates than the transporter (DEP3) and regulatory (DEP6) genes. The phenotype of an experimentally-induced DEP3 mutant of Fusarium did not support the hypothesis that selective retention of DEP3 and DEP6 protects fungi from exogenous depudecin. Together, the results suggest that HGT and gene loss have contributed significantly to DEP cluster distribution, and that some DEP genes provide a greater fitness benefit possibly due to a differential tendency to form network connections.
Abstract
No abstract has been registered
Authors
Marcos Viejo Elena Carneros Hugh Cross Igor A. Yakovlev Carl Gunnar Fossdal Jorunn Elisabeth OlsenAbstract
No abstract has been registered
Abstract
The use of digital aerial photogrammetry (DAP) for forest inventory purposes has been widely studied and can produce comparable accuracy compared with airborne laser scanning (ALS) in small, homogeneous areas. However, the accuracy of DAP for large scale applications with heterogeneous terrain and forest vegetation has not yet been reported. In this study we examined the accuracy of timber volume, biomass and basal area prediction models based on DAP and national forest inventory (NFI) data on a large area in central Norway. Two separate point clouds were derived from aerial image acquisitions of 2010 and 2013. Vegetation heights were extracted by subtracting terrain elevation derived from ALS. A large number of NFI sample plots (483) measured between 2010 and 2014 were used as reference data to fit linear models for timber volume, biomass and basal area with height metrics derived from the DAP data as explanatory variables. Variables describing the heterogeneous environmental and image acquisition conditions were calculated and their influence on the model accuracy was tested. The results showed that forest parameter prediction using DAP works well when applied to a large area. The model fits of the timber volume, biomass and basal area models were good with R2 of 0.80, 0.81, 0.81 and RMSEs of 41.43 m3 ha−1 (55% of the mean observed value), 32.49 t ha−1 (47%), 5.19 m2 ha−1 (41%), respectively. Only a small proportion of the variation could be attributed to the heterogeneous conditions. The inclusion of the relative sun inclination led to an improvement of the model RMSEs by 2% of the mean observed values. The relatively low cost and stability across large areas make DAP an attractive source of auxiliary information for large scale forest inventories.
Abstract
The aim of this study was to evaluate the natural occurrence of Beauveria spp. in soil, from infections in the stink bug Piezodorus guildinii, an important pest of common bean (Phaseolus vulgaris) and as endophytes in bean plant tissue. Twelve conventional and 12 organic common bean fields in the Villa Clara province, Cuba were sampled from September 2014 to April 2015. One hundred and fifty Beauveria isolates were obtained from soil samples, bean plant parts and stink bugs. The overall frequency of occurrence of Beauveria isolates in conventional fields (8.4%) was significantly lower than that in organic fields (23.6%). Beauveria were also obtained significantly more frequently from bean roots in organic fields (15.0%) compared to bean roots in conventional fields (3.3%). DNA sequencing of the intergenic Bloc region was performed for Beauveria species identification. All isolates where characterized as Beauveria bassiana (Balsamo-Crivelli) Vuillemin, and clustered with isolates of neotropical origin previously described as AFNEO_1. The Cuban B. bassiana isolates formed five clusters in the phylogeny. Isolates of two clusters originated from all four locations, organic and conventional fields, as well as soil, plants and stink bugs. Organic fields contained isolates of all five clusters while conventional fields only harbored isolates of the two most frequent ones. Mating type PCR assays revealed that mating type distribution was skewed, with MAT1/MAT2 proportion of 146/4, indicating limited potential for recombination. The present study is the first to report of B. bassiana as a naturally occurring endophyte in common bean. Further, it shows that B. bassiana occurs naturally in diverse environments of common bean fields, and constitutes a potential reservoir of natural enemies against pest insects particularly in organic fields.
Authors
Ingunn M. VågenAbstract
No abstract has been registered
Authors
Nick Hutchings Isabelle Weindl C. F. E. Topp V. O. Snow A. Rotz H Raynal Şeyda Özkan Gülzari R Martin D. P. Holzworth A.-I. Graux P. Faverdin Agustin del Prado Richard Eckard André BanninkAbstract
No abstract has been registered
Authors
Gregory TaffAbstract
No abstract has been registered
Abstract
Application of fish sludge as fertiliser to agricultural land can contribute to closing nutrient cycles in fish farming. The effect of different treatment technologies on the nitrogen fertilisation effects of fish sludge was studied by a bioassay with barley (Hordeum vulgare), an incubation and a field experiment. Dried fish sludge resulted in relative agronomic efficiency of 50–80% compared with mineral fertiliser. The anaerobic digestate based on fish sludge (20 vol%) and dairy manure did not increase nitrogen uptake in barley. Increasing the ratio of fish sludge in the digestate increased the fertilisation effect, but requires optimisation of the biogas process. A simple logistics analysis conducted for a case hatchery showed that on-site drying and co-digestion of fish sludge in a central biogas plant can be regarded as equal in terms of costs. Norway can become an exporter of fish sludge-based recycling fertilisers if current regulations are modified to facilitate nutrient recycling.
Authors
Milan Lstibůrek Yousri A. El-Kassaby Tore Skrøppa Gary R. Hodge Jørn Henrik Sønstebø Arne SteffenremAbstract
No abstract has been registered