Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2017
Authors
Melissa Magerøy Denis Lachance Sharon Jancsik Genevieve Parent Armand Seguin John Mackay Joerg BohlmannAbstract
No abstract has been registered
Abstract
Plants are sessile organisms that lack a specialized immune system to cope with biotic and abiotic stress. Instead, plants have complex regulatory networks that determine the appropriate distribution of resources between the developmental and the defense programs. In the last years, epigenetic regulation of repeats and gene expression has evolved as an important player in the transcriptional regulation of stress‐related genes. Here, we review the current knowledge about how different stresses interact with different levels of epigenetic control of the genome. Moreover, we analyze the different examples of transgenerational epigenetic inheritance and connect them with the known features of genome epigenetic regulation. Although yet to be explored, the interplay between epigenetics and stress resistance seems to be a relevant and dynamic player of the interaction of plants with their environments.
Authors
Melissa MagerøyAbstract
No abstract has been registered
Authors
Melissa MagerøyAbstract
No abstract has been registered
Authors
Melissa MagerøyAbstract
No abstract has been registered
Authors
Shuxin Han Yanling Gao Guoquan Fan Wei Zhang Cailing Qiu Shu Zhang Yanju Bai Junhua Zhang Carl Jonas Jorge SpetzAbstract
This study reports the findings of a distinct Potato virus Y (PVY) isolate found in Northeast China. One hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of Harbin in Heilongjiang province of China. The collected tubers were planted and let to grow in a greenhouse. New potato plants generated from these tubers showed similar symptoms, except for one plant. Subsequent serological analyses revealed PVY as the causing agent of the disease. A novel PVY isolate (referred to as HLJ-C-44 in this study) was isolated from this sample showing unique mild mosaic and crisped leaf margin symptoms. The complete genome of this isolate was analyzed and determined. The results showed that HLJ-C-44 is a typical PVY isolate. Phylogenetic analysis indicated that this isolate belongs to the N-Wi strain group of PVY recombinants (PVYN-Wi) and also shared the highest overall sequence identity (nucleotide and amino acid) with other members of this strain group. However, recombination analysis of isolate HLJ-C-44 revealed a recombination pattern that differed from that of other PVYN-Wi isolates. Moreover, biological assays in four different potato cultivars and in Nicotiana tabacum also revealed a different phenotypic response than that of a typical PVYN-Wi isolate. This data, combined, suggest that HLJ-C-44 is a novel PVY recombinant with distinct biological properties.
Authors
Umberto Salvagnin Mickael Malnoy Gunda Thöming Marco Tasin Silvia Carlin Stefan Martens Urska Vrhovsek Sergio Angeli Gianfranco AnforaAbstract
Herbivorous insects use olfactory cues to locate their host plant within a complex olfactory landscape. One such example is the European grapevine moth Lobesia botrana, a key pest of the grape in the Palearctic region, which recently expanded both its geographical and host plant range. Previous studies have showed that a synthetic blend of the three terpenoids, (E)-β-caryophyllene, (E)-β-farnesene and (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), was as attractive for the moth as the complete grape odour profile in laboratory conditions. The same studies also showed that the specific ratio of these compounds in the grape bouquet was crucial because a percentage variation in any of the three volatiles resulted in almost complete inhibition of the blend's attractiveness. Here, we report on the creation of stable grapevine transgenic lines, with modified (E)-β-caryophyllene and (E)-β-farnesene emission and thus with an altered ratio compared to the original plants. When headspace collections from these plants were tested in wind tunnel behavioural assays, they were less attractive than control extracts. This result was confirmed by testing synthetic blends imitating the ratio found on natural and transformed plants, as well as by testing the plants themselves. With this evidence, we suggest that a strategy based on volatile ratio modification may also interfere with the host-finding behaviour of L. botrana in the field, creating avenues for new pest control methods.
Authors
Umberto Salvagnin M. Malnoy Gunda Thöming M. Tasin S. Carlin S. Martens U. Vrhovsek S. Angeli G. AnforaAbstract
In nature plant terpenoids play multiple ecological roles. Many phytophagous insects use them as kairomones to locate their host plants. This is also the case for Lobesia botrana, which is the main pest of European vineyards. It was found that a specific blend of the terpenoids (E)-β-caryophyllene, (E)-β-farnesene and the homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene emitted by grapevine was attractive to L. botrana females, and the attractiveness was shown to be dependent on the kairomone ratio. In this work, we generated stable grapevine transgenic lines with altered (E)-β-caryophyllene and (E)-β-farnesene emission compared to natural plants. Thus, we modified the ratio between these two kairomones in vivo, and tested how it affected L. botrana behaviour.
Authors
Stig A. BorgvangAbstract
No abstract has been registered
Authors
Stig A. BorgvangAbstract
No abstract has been registered