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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2023

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Abstract

Birch wood is a potential feedstock for biogas production in Northern Europe; however, the lignocellulosic matrix is recalcitrant preventing efficient conversion to methane. To improve digestibility, birch wood was thermally pre-treated using steam explosion at 220 °C for 10 min. The steam-exploded birch wood (SEBW) was co-digested with cow manure for a period of 120 days in continuously fed CSTRs where the microbial community adapted to the SEBW feedstock. Changes in the microbial community were tracked by stable carbon isotopes- and 16S r RNA analyses. The results showed that the adapted microbial culture could increase methane production up to 365 mL/g VS day, which is higher than previously reported methane production from pre-treated SEBW. This study also revealed that the microbial adaptation significantly increased the tolerance of the microbial community against the inhibitors furfural and HMF which were formed during pre-treatment of birch. The results of the microbial analysis indicated that the relative amount of cellulosic hydrolytic microorganisms (e.g. Actinobacteriota and Fibrobacterota) increased and replaced syntrophic acetate bacteria (e.g. Cloacimonadota, Dethiobacteraceae, and Syntrophomonadaceae) as a function of time. Moreover, the stable carbon isotope analysis indicated that the acetoclastic pathway became the main route for methane production after long-term adaptation. The shift in methane production pathway and change in microbial community shows that for anaerobic digestion of SEBW, the hydrolysis step is important. Although acetoclastic methanogens became dominant after 120 days, a potential route for methane production could also be a direct electron transfer among Sedimentibacter and methanogen archaea.

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Abstract

We conducted a study over four rice seasons to assess the effects of dairy manure application on water loss, nutrient leaching, and rice yield compared to chemical fertilization. Water input, soil water storage, water percolation, plant growth, and yield data were recorded under triplicate field lysimeters that received either chemical fertilizers or organic manure. The lysimeters received alternate wetting and drying irrigation (5-cm after 3 days (2018 Aman season), 6 days (2019 Boro and Aman seasons), and 9 days (2020 Boro season) of ponded water disappearance) in addition to rainfall (37.5, 33.1, 40.9, and 47.4 cm, respectively). Leachate and ponded water samples were analyzed for nitrogen (N) species (NH4+ - N and NO3− - N) and available phosphorus (P) content. Manure application increased soil water storage by 1.2–4.4 cm/m but did not affect percolation loss (44–64% of water input) in silt loam soil. The chemical fertilization had significantly higher leaching concentrations of nutrients (NO3− - N at 0.75–3.6 mg/L and P at 0.02–0.15 mg/L) in several leaching events in the last three seasons than the manure treatment (NO3− - N at 0.75–3.2 mg/L and P at 0–0.21 mg/L). Overall, the manure treatment reduced the leaching load of N and available P by 13% and 23.6%, respectively. The N and P concentrations in the topsoil were higher for the manure treatment. Manure application increased rice yield by 15% and water productivity by 0.07 kg/m3 by augmenting soil water availability during the drying cycles of alternate wetting and drying processes. In addition, recycling manure in soil significantly reduced its environmental pollution compared to other inappropriate disposal methods. However, research needs remain important to adjust manure management options.

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Abstract

Objective: Precision livestock farming technologies show great promise for the management of extensive, arid rangelands, but more practical knowledge is needed to allow ranchers to determine potential applications and limitations for adoption. We tested a long-range wide area network (LoRa-WAN) precision livestock system over 3 mo (April–June 2020) in a ranch in southwest New Mexico, USA. The system monitors cattle position and movements, precipitation, and water trough water levels at pasture and ranch scales, in real time. Materials and Methods: Here we describe the components of the system and share what we have learned from our preliminary experiences. This system included a solar-power LoRa-WAN receiving station with the corresponding gateway, radio frequency antenna (824–894 MHz), and Wi-Fi bridge for data transmission into the Internet. The testbed network for testing LoRa-WAN sensors included 43 GPS-trackers deployed on lactating beef cows and 2 environmental sensors used to monitor precipitation regimens and trough water levels, respectively. Results and Discussion: The system collected data consistently for the trough levels and precipitation, whereas data from the cow GPS-trackers was highly heterogeneous. On average, 46 ± 4% of daily data packets logged by GPS-trackers were successfully transmitted through the LoRa-WAN system, exceeding 80% of successful transmission in several cases. This report documents the necessary infrastructure, performance, and maintenance of system components, which could be of significant information value to ranchers and researchers with a desire to deploy similar monitoring systems. Implications and Applications: This Technical Note documents the implemetation of a LoRa-WAN monitoring system at the ranch scale for a 3-mo period. The system has allowed the ranch manager and assisting staff to efficiently manage cattle inventories and promptly address animal welfare concerns. However, further research is required to assess the scalability of this system across commercial operating cattle ranches in the Southwest United States, thereby unlocking its potential for broader adoption and effect.

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Abstract

A more plant-based diet will contribute to food sustainability. Achieving this change requires collaboration across disciplines which is not easy to achieve. This article illustrates how interdisciplinary collaboration in a large research project can be facilitated through a design-led innovation process juxtaposing approaches from design and science. Consumer insights were used in creative workshops to ideate and develop packaging and product concepts for plant-based food focusing on ‘environment’, ‘health’ and ‘Norwegian’ design imperatives. Learning loops of alignment – creation – feedback were applied to design and test six packaging prototypes of two product categories (Pea Porridge, Faba Bean Drink). Qualitative feedback was collected from 147 consumers and a quantitative survey with 1102 Norwegian consumers tested product expected liking and product-concept match. Younger consumers and users of plant-based products exhibited a higher expected liking vs. non-users and older respondents. Packaging design adopted for specific consumer segments can positively contribute to a shift to more plant-based diets. We show how a dynamic interdisciplinary innovation approach can be powerful to creating new product ideas, getting consumers’ input and fostering collaboration and learning among disciplines. We offer other researchers and the food industry actionable opportunity areas and design imperatives for their innovation activities around plant-based food.

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Abstract

European canker is one of the most devastating fungal diseases of apple in most temperate regions. The causal agent, Neonectria ditissima, infects trees through wounds in the bark forming cankers that girdle the stem and eventually cause tree death. Timely protection of the trees is challenged by stagnation of symptom expression after infections for a long period of time. The objective of this research is to use a novel TaqMan PCR assay to detect and quantify N. ditissima during the asymptomatic colonization of apple wood. Pruning wounds on branches of the cultivars Elstar and Gala were inoculated with N. ditissima and wood discs were sampled at 2–6, 10–14, and 30–34 mm distance from the inoculation site after 3 hours, 2 weeks, 4 weeks and 8 weeks for the detection and quantification of the pathogen. The TaqMan PCR assay detected N. ditissima in 51% of the inoculated apple tree samples. This was more sensitive than the culturing method detecting N. ditissima in 11% of the samples. An accumulation of N. ditissima DNA up to 34 mm distance from the inoculation site was observed without development of visible symptoms. To our knowledge this is the first time colonization of N. ditissima was detected and quantified in the absence of symptoms of European canker. The implications of this research are discussed.