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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2008

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Abstract

The present cross-sectional study was performed to analyze potential risk factors for upper extremity disorders in two groups of forest machine operators driving harvesting vehicles and performing equal tasks in France (n=18) and Norway (n=19). This comparative design implied similar work tasks, but potentially different external work demands. Previous studies have suggested higher levels of neck and shoulder complaints among Norwegian operators compared to those of the French. This may be related to different external work demands and/or individual motor performance. Surface electromyography (EMG) of the right upper trapezius (RUT) and extensor digitorum (RED) muscles were measured continuously during 1 working day (7.5–8 h per operator) and video of body postures was recorded inside the cabin (1 h per operator). A questionnaire on external work demand factors and psychosocial stressors was used together with the collection of symptom data. A physical examination was performed on all workers. Borg's CR-10 scale of intensity of discomfort/pain was rated four times throughout the test day. The French operators reported less complaint (p<0.01) in the right neck compared to their Norwegian colleagues. Furthermore, the French had on average two to three times longer lunch breaks during 5 work days, less continuous hand intensive use of the control lever with more frequent short breaks during the test day compared to the Norwegian operators. However, the average static load level on the RUT muscle for the whole working day was significantly higher among the French (0.7% EMGmax) in comparison with the Norwegian operators (0.3% EMGmax). No difference was found in number of periods with sustained low-level muscle activity neither in the RUT nor the RED muscle. In conclusion, our results support that the higher prevalence of discomfort/pain among the Norwegian forest machine operators may be related to organizational factors.

Abstract

The area of wood protection is in a period of change. New wood protection systems have been developed while their mode of action remains insufficiently understood. The development of molecular methods provides potential tools to investigate the interaction between modified wood and decay fungi. One small step to tackle some of the unsolved questions about the mode of action of modified wood is taken in this study. A specific and quantitative real-time PCR (QRT-PCR) assay was now established for identifying and quantifying early stages of fungal colonisation in modified wood and for profiling growth dynamics of the white-rot fungus Trametes versicolor through different stages of decay. QRT-PCR of colonisation of three different wood modification systems (acetylation, furfurylation, thermal modification), two reference treatments (Cu-HDO, CCA) and Scots pine sapwood as control was performed. Incubation time was 2, 4, 6, 8 and 10 weeks...

Abstract

Agrobacterium-mediated transformation for poinsettia (Euphorbia pulcherrima Willd. Ex Klotzsch) is reported here for the first time. Internode stem explants of poinsettia cv. Millenium were transformed by Agrobacterium tumefaciens, strain LBA 4404, harbouring virus-derived hairpin (hp) RNA gene constructs to induce RNA silencing-mediated resistance to Poinsettia mosaic virus (PnMV). Prior to transformation, an efficient somatic embryogenesis system was developed for poinsettia cv. Millenium in which about 75% of the explants produced somatic embryos. In 5 experiments utilizing 868 explants, 18 independent transgenic lines were generated. An average transformation frequency of 2.1% (range 1.2-3.5%) was revealed. Stable integration of transgenes into the poinsettia nuclear genome was confirmed by PCR and Southern blot analysis. Both single- and multiple-copy transgene integration into the poinsettia genome were found among transformants. Transgenic poinsettia plants showing resistance to mechanical inoculation of PnMV were detected by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Northern blot analysis of low molecular weight RNA revealed that transgene-derived small interfering (si) RNA molecules were detected among the poinsettia transformants prior to inoculation. The Agrobacterium-mediated transformation methodology developed in the current study should facilitate improvement of this ornamental plant with enhanced disease resistance, quality improvement and desirable colour alteration. Because poinsettia is a non-food, non-feed plant and is not propagated through sexual reproduction, this is likely to be more acceptable even in areas where genetically modified crops are currently not cultivated.

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Abstract

Sweet potato (Ipomoea batatas L.) is the fifth most important crop in the developing countries after rice, wheat, maize and cassava. The amplified fragment length polymorphism (AFLP) method was used to study the genetic diversity and relationships of sweet potato accessions in the germplasm collection of Sokoine University of Agriculture, Morogoro and Sugarcane Research Institute, Kibaha, Tanzania. AFLP analysis of 97 sweet potato accessions using ten primer combinations gave a total of 202 clear polymorphic bands. Each one of the 97 sweet potato accessions could be distinguished based on these primer combinations. Estimates of genetic similarities were obtained by the Dice coefficient, and a final dendrogram was constructed with the un-weight pair-group method using arithmetic average. AFLP-based genetic similarity varied from 0.388 to 0.941, with a mean of 0.709. Cluster analysis using genetic similarity divided the accessions into two main groups suggesting that there are genetic relationships among the accessions. Principal Coordinate analysis confirmed the pattern of the cluster analysis. Analysis of molecular variance revealed greater variation within regions (96.19%) than among regions (3.81%). The results from the AFLP analysis revealed a relatively low genetic diversity among the germplasm accessions and the genetic distances between regions were low. A maximally diverse subset of 13 accessions capturing 97% of the molecular markers diversity was identified. We were able to detect duplicates accessions in the germplasm collection using the highly polymorphic markers obtained by AFLP, which were found to be an efficient tool to characterize the genetic diversity and relationships of sweet potato accessions in the germplasm collection in Tanzania.

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Abstract

A sample selection framework that simultaneously takes into account the two-step decision-making of forest owners (first whether or not to harvest, second the level of harvesting) is applied on representative cross-sectional data for forest properties and owners in Norway. Forest management plans, property size, forested area and income from agriculture are found to increase both the propensity to harvest and the harvesting levels. Income from engagement in other outfield-related productions and debt burden increase the propensity to harvest only, while increased age impact negatively on the harvesting decision. Wage income affects both propensities to harvest and harvesting levels negatively. The results suggest that other on-property productions may stimulate harvesting decisions, while off-property income impact harvesting decisions and levels negatively. (C) 2007 Elsevier GmbH. All rights reserved.

Abstract

New restrictions draw governments, industry and research towards new and environmental benign wood protective agents. These agents often come from a natural source, and are also a waste product. One of these is chitosan. Chitosan is a derivative from chitin, mainly found in the exoskeleton of crustacean. Some research has been conducted on chitosan and wood. Chitosan has earlier proven good antifungal effectiveness, but to achieve a good protection, a 5 % concentration is needed, which makes the end product quite expensive. In recent research, a way to make chitosan treated wood hydrophobic has been invented. The objective of the research presented in this paper, is to describe the effectiveness of the following compounds: Chitosan, chitosan/copper, chitosan/boron and chitosan/Scanimp (a commercial wood preservative). Results show that chitosan works well alone, but has a fixation problem. Chitosan and boron give good fixation, and reduced amounts of chitosan and together give good protection against wood destroying fungi. The fire protection agents are promising, but needs higher concentration and/or better fixation to give a sufficient protection against wood destroying fungi