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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

NIBIO's Scientific Publications →

2020

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Authors

Fatima Heinicke Xiangfu Zhong Karola Manuela Zucknick Johannes Breidenbach Arvind Yegambaram Meenakshi Sundaram Siri Tennebø Flåm Magnus Leithaug Marianne Dalland Simon Rayner Benedicte Alexandra Lie Gregor Gilfillan

Abstract

High-throughput sequencing has emerged as the favoured method to study microRNA (miRNA) expression, but biases introduced during library preparation have been reported. We recently compared the performance (sensitivity, reliability, titration response and differential expression) of six commercially-available kits on synthetic miRNAs and human RNA, where library preparation was performed by the vendors. We hereby supplement this study with data from two further commonly used kits (NEBNext, NEXTflex) whose manufacturers initially declined to participate. NEXTflex demonstrated the highest sensitivity, which may reflect its use of partially-randomized adapter sequences, but overall performance was lower than the QIAseq and TailorMix kits. NEBNext showed intermediate performance. We reaffirm that biases are kit specific, complicating the comparison of miRNA datasets generated using different kits.

Authors

Trygve S. Aamlid Karin Juul Hesselsøe

Abstract

No abstract has been registered

Authors

Trygve S. Aamlid G. Jagger G. R. Tuxen J. Bentley M. Thers

Abstract

No abstract has been registered

Authors

Callum Aidan Stephen Hill Gry Alfredsen Mark Hughes Lone Ross

Abstract

No abstract has been registered

Authors

Olaf Skurdal Tofte Lone Ross Dag Fjeld

Abstract

No abstract has been registered

Authors

Christian Kanzian Thomas Holzfeind Lone Ross Karin Westlund Dag Fjeld

Abstract

No abstract has been registered

Authors

Tore Skrøppa

Abstract

No abstract has been registered

Authors

Trygve S. Aamlid Pia Heltoft Thomsen Tatsiana Espevig Gudni Thorvaldsson Anne Mette Dahl Jensen Karin Juul Hesselsøe Wendy Marie Waalen T. K. Petersen Trond Olav Pettersen Jan Tangsveen P. Sørensen Tania Gneist Bjarni Hannesson

Abstract

No abstract has been registered

Authors

Trygve S. Aamlid Anne Falk Øgaard Karin Juul Hesselsøe T. Krogstad M. Woods Yajun Chen K. Sintorn N. Dokkuma Wolfgang Prämassing D. Cleaver

Abstract

No abstract has been registered

Authors

Hans Martin Hanslin Trygve S. Aamlid J. Kollmann T. Lennartson Ellen Johanne Svalheim Eveliina Kallioniemi

Abstract

No abstract has been registered