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Publications

NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.

2019

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Abstract

Shallot (Allium cepa var. aggregatum) is an important vegetable crop belonging to the genus Allium. The present study attempted to develop an efficient droplet-vitrification cryopreservation method for shallot ‘10603’ shoot tips. In vitro stock shoots were maintained on Murashige and Skoog (1962) medium (MS) supplemented with 30 g L-1 sucrose, 0.5 mg L-1 BAP, 0.1 mg L-1 NAA and 8 g L-1 agar (pH=5.8). Shoot tips (2.0-3.0 mm in length) were excised from 4-week-old stock shoots and stepwise precultured with increased sucrose concentrations from 0.3 to 0.5 M, each concentration for 1 day. The precultured shoot tips were then loaded for 20 min with a solution composed of 2 M glycerol and 0.5 M sucrose, before exposure to PVS3 for 3 h at room temperature. Dehydrated shoot tips were transferred onto aluminum foils (2×0.8 cm), prior to direct immersion into liquid nitrogen (LN) for cryostorage. For thawing, frozen aluminum foils were moved from LN and immediately transferred into unloading solution composed of liquid MS containing 1.2 M sucrose. After incubation at room temperature for 20 min, shoot tips were post-cultured on solidified MS medium containing 0.3 M sucrose for 2 days and then transferred onto a recovery medium for shoot regrowth. With this procedure, 94% shoot tips survived, and 58% shoot tips regenerated into shoots following cryopreservation.

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Abstract

Soil is one of the most species-rich habitats and plays a crucial role in the functioning of terrestrial ecosystems. It is acknowledged that soils and their biota deliver many ecosystem services. However, up to now, cultural ecosystem services (CES) provided by soil biodiversity remained virtually unknown. Here we present a multilingual and multisubject literature review on cultural benefits provided by belowground biota in European forests. We found 226 papers mentioning impact of soil biota on the cultural aspects of human life. According to the reviewed literature, soil organisms contribute to all CES. Impact on CES, as reflected in literature, was highest for fungi and lowest for microorganisms and mesofauna. Cultural benefits provided by soil biota clearly prevailed in the total of the reviewed references, but there were also negative effects mentioned in six CES. The same organism groups or even individual species may have negative impacts within one CES and at the same time act as an ecosystem service provider for another CES. The CES were found to be supported at several levels of ecosystem service provision: from single species to two or more functional/taxonomical groups and in some cases morphological diversity acted as a surrogate for species diversity. Impact of soil biota on CES may be both direct – by providing the benefits (or dis-benefits) and indirect – through the use of the products or services obtained from these benefits. The CES from soil biota interacted among themselves and with other ES, but more than often, they did not create bundles, because there exist temporal fluctuations in value of CES and a time lag between direct and indirect benefits. Strong regionality was noted for most of CES underpinned by soil biota: the same organism group or species may have strong impact on CES (positive, negative or both) in some regions while no, minor or opposite effects in others. Contrarily to the CES based on landscapes, in the CES provided by soil biota distance between the ecosystem and its CES benefiting area is shorter (CES based on landscapes are used less by local people and more by visitors, meanwhile CES based on species or organism groups are used mainly by local people). Our review revealed the existence of a considerable amount of spatially fragmented and semantically rich information highlighting cultural values provided by forest soil biota in Europe.

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Abstract

Semi-natural grasslands are hotspots of biodiversity in Europe and provide amounts of flower resources for pollinators. We present data on composition and spatial turnover of herb species and flower resources in and between semi-natural grasslands in Romania mown at different times during the growth season (early, intermediate, late). The data include herb species occurrences, their phenological stage, flower resources, and measures of spatial turnover of the species occurrences and flower resources based on Detrended Correspondence Analyses (DCA), in the start of August. The dataset is provided as supplementary material and associated with the research article “Traditional semi-natural grassland management with heterogeneous mowing times enhances flower resources for pollinators in agricultural landscapes” [1] Johansen et al.. See Johansen et al. for data interpretation.