Publications
NIBIOs employees contribute to several hundred scientific articles and research reports every year. You can browse or search in our collection which contains references and links to these publications as well as other research and dissemination activities. The collection is continously updated with new and historical material.
2022
Abstract
Fecal contamination of water constitutes a serious health risk to humans and environmental ecosystems. This is mainly due to the fact that fecal material carries a variety of enteropathogens, which can enter and circulate in water bodies through fecal pollution. In this respect, the prompt identification of the polluting source(s) is pivotal to guiding appropriate target-specific remediation actions. Notably, microbial source tracking (MST) is widely applied to determine the host origin(s) contributing to fecal water pollution through the identification of zoogenic and/or anthropogenic sources of fecal environmental DNA (eDNA). A wide array of host-associated molecular markers have been developed and exploited for polluting source attribution in various aquatic ecosystems. This review is intended to provide the most up-to-date overview of genetic marker-based MST studies carried out in different water types, such as freshwaters (including surface and groundwaters) and seawaters (from coasts, beaches, lagoons, and estuaries), as well as drinking water systems. Focusing on the latest scientific progress/achievements, this work aims to gain updated knowledge on the applicability and robustness of using MST for water quality surveillance. Moreover, it also provides a future perspective on advancing MST applications for environmental research.
Abstract
Zoogenic faecal contamination of the environment is one of the indices included in the evaluation of ecological threats, health hazards and adverse impacts on various ecosystems. The risks and environmental concerns are associated with the fact that faeces of wild and domesticated animals constitute the largest source of environmental loading of enteropathogens associated with transmission of zoonotic diseases (enteric zoonoses). Although sick animals are more likely to transmit pathogens, healthy ones can also be the carriers and defecate them into the environment. This is of particular importance given the close human-animal interactions and health effects resulting from human and ecological exposures to faecal hazards from companion and farm animals. We have therefore set out to investigate whether healthy equines can carry and defecate human infectious pathogens. For this purpose, we set up a pilot study to examine the faecal DNA of horses using culture-independent molecular diagnostics – fluorescent probe-based quantitative real-time PCR. Our results revealed that among a total of 23 horses, 6 were found to carry Campylobacter jejuni (C. jejuni), and 5 had Salmonella enterica serovar Typhimurium (S. Typhimurium). Moreover, Enterococcus faecalis (E. faecalis) was found in 14 horses, while 19 were positive for Clostridium perfringens (C. perfringens). Furthermore, the frequently reported protozoan parasites in livestock, Cryptosporidium parvum (C. parvum) and Giardia lamblia (G. lamblia), were discovered in 8 and 7 samples, respectively. This pilot study shed new light on the phenomenon of healthy horses carrying C. jejuni and other human-health-related enteropathogens.
Abstract
No abstract has been registered
Authors
Paul Eric Aspholm Juho Vuolteenaho Hallvard Jensen Cornelya Klutsch Helena Klöckener Snorre HagenAbstract
No abstract has been registered
Abstract
No abstract has been registered
Authors
Mari Røken Kristin Forfang Yngvild Wasteson Anita Haug Haaland Hans Geir Eiken Snorre Hagen Ane Mohn BjellandAbstract
Aims To investigate and compare antimicrobial resistance genes (ARGs) in faeces from cohabiting dogs and owners. Methods and Results DNA from faecal samples from 35 dogs and 35 owners was screened for the presence of 34 clinically relevant ARGs using high throughput qPCR. In total, 24 and 25 different ARGs were present in the dog and owner groups, respectively. The households had a mean of 9.9 ARGs present, with dogs and owners sharing on average 3.3 ARGs. ARGs were shared significantly more in households with dogs over 6 years old (3.5, interquartile range 2.75–5.0) than in households with younger dogs (2.5, interquartile range 2.0–3.0) (p = 0.02). Dogs possessed significantly more mecA and aminoglycoside resistance genes than owners. Conclusions Dogs and owners can act as reservoirs for a broad range of ARGs belonging to several antimicrobial resistance classes. A modest proportion of the same resistance genes were present in both dogs and owners simultaneously, indicating that ARG transmission between the dog and human gut is of minor concern in the absence of antimicrobial selection. Significance and Impact of the Study This study provides insight into the common dog and human gut resistomes, contributing to an improved knowledge base in risk assessments regarding ARG transmission between dogs and humans.
Abstract
No abstract has been registered
Authors
Claire Coutris Erik J. Joner Cecilie Singdahl-Larsen Ana Catarina Almeida Muhammad Umar Sissel Brit Ranneklev Cecilia AskhamAbstract
Microplastics ending up in nature as a result of end-of-life processes for plastic packaging is a serious environmental concern, and was addressed in the Packnoplast project through sampling at three sites: one biogas facility in Norway and two thermoplastic recycling plants, one in Norway and one in The Netherlands. The amounts of microplastics ending up in soil from biogas digestate was estimated to represent 0.4-2 mg/kg soil per year if 6 t/daa of biogas digestate is used as fertilizer. Food packaging is estimated to represent 75% of this. The amounts of microplastics measured are significant, but too small to affect soil properties even on a time-scale of decades. The risk of adverse effects on soil quality, plant growth or soil organisms seem very low at the current predicted rates of plastic inputs to soil. Since plastics are virtually non-degradable, they are still prone to accumulate in soil, and waste streams recycled to soil need to address and prevent plastic contamination even better than today. Thermoplastic recycling plants are handling large amounts of plastic, and during processes in the plant, microplastics are generated. Concentrations of microplastic particles varied from 7 to 51 particles per lite rin the effluent water from the two plants. Discharges of effluent water are often through the sewer system and/or into a water body. Today regulations regarding discharges of microplastics are missing. Sand filter treatment of the effluent water was a promising treatment technique to remove the microplastics. Background concentrations of microplastics, comparable to pristine areas, were found in blue mussels sampled outside the thermoplastic recycling plant in Norway. Knowledge about the risk imposed by microplastics to the aquatic environment is today not known.
Abstract
Prediction of the relative phosphorus (P) fertiliser value of bio-based fertiliser products is agronomically important, but previous attempts to develop prediction models have often failed due to the high chemical complexity of bio-based fertilisers and the limited number of products included in analyses. In this study, regression models for prediction were developed using independently produced data from 10 different studies on crop growth responses to P applied with bio-based fertiliser products, resulting in a dataset with 69 products. The 69 fertiliser products were organised into four sub-groups, based on the inorganic P compounds most likely to be present in each product. Within each product group, multiple regression was conducted using mineral fertiliser equivalents (MFE) as response variable and three potential explanatory variables derived from chemical analysis, all reflecting inorganic P binding in the fertiliser products: i) NaHCO3-soluble P, ii) molar ratio of calcium (Ca):P and iii) molar ratio of aluminium+iron (Al+Fe):P. The best regression model fit was achieved for sewage sludges with Al-/Fe-bound P (n = 20; R2 = 79.2%), followed by sewage sludges with Ca-bound P (n = 11; R2 = 71.1%); fertiliser products with Ca-bound P (n = 29; R2 = 58.2%); and thermally treated sewage sludge products (n=9;R2=44.9%). Even though external factors influencing P fertiliser values (e.g. fertiliser shape, application form, soil characteristics) differed between the underlying studies and were not considered, the suggested prediction models provide potential for more efficient P recycling in practice.
Authors
Yong Q. Tian Qian Yu Hunter J. Carrick Brian L. Becker Remegio Confesor Mark Francek Olivia C. AndersonAbstract
No abstract has been registered